A collaboration of aquaporins handles water transport in relation to the estrous cycle in the bitch uterus

Aralla, Marina; Borromeo, V.; Groppetti, D.; Secchi, C.; Crémonesi, F.; Arrighi, S.

doi:10.1016/j.theriogenology.2009.01.023

Cited by 32 publications

(32 citation statements)

References 37 publications

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“…Our immunohistochemical results did not show AQP1 staining in the smooth muscle cells but indicated its presence in the myometrial vessels, which is consistent with our previous studies performed with cyclic pigs [11]. However, recently Aralla et al [18] demonstrated the expression of AQP1 in the uterine blood vessels and also in the myometrium of cycling bitches. Moreover, in this study, the AQP1 localization and expression were not affected by stages of the estrous cycle [18], like in the pig, as demonstrated in the present study.…”

Section: Discussionsupporting

confidence: 91%

“…Recently, nitric oxide synthase 3 (NOS3) was localized in the myometrial and epithelial tissues as well as in blood vessels indicating a contribution of this vasoactive peptide to the uterine imbibition processes [18]. However, further studies are required to substantiate the hypotheses of LH, PGs and NO roles in regulation of AQPs in the uterus.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Skowronski et al [11] showed the expression of AQP1 in the endothelial cells of the uterine blood vessels, AQP5 in the myometrium, as well as AQP5 and AQP9 in luminal and glandular epithelium. Moreover, AQP1, AQP2, and AQP5 have been identified in the bitch uterus [18]. AQP2 expression was also found in human endometrial cells of the uterus [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Distribution and quantitative changes in amounts of aquaporin 1, 5 and 9 in the pig uterus during the estrous cycle and early pregnancy

Skowroński

2010

Reprod Biol Endocrinol

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BackgroundAquaporins (AQPs) are a family of membrane channel proteins that facilitate bulk water transport. To date, 11 isoforms of AQPs have been reported to be expressed in the female and male reproductive systems. The purpose of our study was to determine the localization and quantitative changes in the expression of AQP1, 5 and 9 within the pig uterus during different stages of the estrous cycle and early pregnancy.MethodsImmunoperoxidase and semi-quantitative immunoblotting techniques were used to examine the distribution and changes in amounts of AQP1, AQP5 and AQP9 in uteral cells of pigs at the early (Days 2-4), middle (10-12), late (14-16) stage of the luteal phase and late (18-20) stage of the follicular phase of the estrous cycle as well as on Days 14-16 and 30-32 of gestation (the onset and the end of implantation process).ResultsThe results demonstrated that AQP1, 5, and 9 were clearly detected in all studied stages of the estrous cycle and pregnancy. AQP1 was localized within uterine blood vessels. In cyclic gilts, endometrial and myometrial expression of AQP1 protein did not change significantly but increased during gestation. AQP5 was localized in smooth muscle cells and uterine epithelial cells. Endometrial expression of AQP5 protein did not change significantly between Days 2-4 and 10-12 of the estrous cycle but increased on Days 14-16 and 18-20 as well as during early pregnancy. Myometrial expression of AQP5 did not differ significantly during the estrous cycle but increased in the pregnancy. The anti-AQP9 antibody labeled uterine epithelial cells of uterus. Endometrial expression of AQP9 did not change significantly between Days 2-4 and 10-12 of the estrous cycle but increased on Days 14-16 and 18-20 as well as during early pregnancy.ConclusionsThe results suggest that a functional and distinctive collaboration exists among diverse AQPs in water handling during the different uterine phases in the estrous cycle and early pregnancy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution and quantitative changes in amounts of aquaporin 1, 5 and 9 in the pig uterus during the estrous cycle and early pregnancy

Skowroński

2010

Reprod Biol Endocrinol

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“…Former studies identified the estrogen response element in the promoter region of the AQP2 and 5 genes, which provides the immediate regulation of these water channels by estrogen [13,14]. Water channel regulation was also investigated in the uterus of cycling bitches, and it was discovered that AQP5 was particularly expressed in response to high levels of progesterone [15]. Skowronska et al [16] also investigated the changes of AQP5 expression due to progesterone and estradiol treatments in an in vitro study on porcine uterus.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Female Sexual Hormones on the Expression of Aquaporin 5 in the Late-Pregnant Rat Uterus

Csányi

Bóta

Falkay

et al. 2016

IJMS

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Thirteen mammalian aquaporin (AQP) water channels are known, and few of them play a role in the mammalian reproductive system. In our earlier study, the predominance of AQP5 in the late-pregnant rat uterus was proven. Our current aim was to investigate the effect of estrogen- and gestagen-related compounds on the expression of the AQP5 channel in the late-pregnant rat uterus. Furthermore, we examined the effect of hormonally-induced preterm delivery on the expression of AQP5 in the uterus. We treated pregnant Sprague-Dawley rats subcutaneously with 17β-estradiol, clomiphene citrate, tamoxifen citrate, progesterone, levonorgestrel, and medroxyprogesterone acetate. Preterm delivery was induced by subcutaneous mifepristone and intravaginal prostaglandin E2. Reverse-transcriptase PCR and Western blot techniques were used for the detection of the changes in AQP5 mRNA and protein expressions. The amount of AQP5 significantly increased after progesterone and progesterone analogs treatment on 18 and 22 days of pregnancy. The 17β-estradiol and estrogen receptor agonists did not influence the AQP5 mRNA level; however, estradiol induced a significant increase in the AQP5 protein level on the investigated days of gestation. Tamoxifen increased the AQP5 protein expression on day 18, while clomiphene citrate was ineffective. The hormonally-induced preterm birth significantly decreased the AQP5 level similarly to the day of delivery. We proved that AQP5 expression is influenced by both estrogen and progesterone in the late-pregnant rat uterus. The influence of progesterone on AQP5 expression is more predominant as compared with estrogen.

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“…At least 12 different tumor cell types have been reported to express AQPs in vivo in humans and rodents. Accumulating evidence suggests that ovarian steroids may affect the expression of several AQPs in the female genital tract (2)(3). AQP-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring, embryonic development and other events requiring cell movement (4).…”

Section: Introductionmentioning

confidence: 99%

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

Jiang

Yan

et al. 2012

Oncology Letters

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Abstract. Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer. We recently demonstrated that ovarian steroids may affect the expression of AQP5 in the female genital tract. In this study, we considered whether AQP5 may affect cell migration in Ishikawa cells, an adenocarcinoma cell line derived from the endometrium. The results showed that the downregulation of AQP5 results in reduced Ishikawa cell migration. The estrogen (E2) receptor in the promoter of AQP5 mediated the regulation of AQP5 expression in the normal endometrium and endometrial cancer. By contrast, the upregulation of AQP5 by E2 increased cell migration, invasion and adhesion through increased annexin-2, which is responsible for F-actin remodeling and rearrangement. E2 regulates Ishikawa cell migration by regulating the AQP5 expression.

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A collaboration of aquaporins handles water transport in relation to the estrous cycle in the bitch uterus

Cited by 32 publications

References 37 publications

Distribution and quantitative changes in amounts of aquaporin 1, 5 and 9 in the pig uterus during the estrous cycle and early pregnancy

Distribution and quantitative changes in amounts of aquaporin 1, 5 and 9 in the pig uterus during the estrous cycle and early pregnancy

The Effects of Female Sexual Hormones on the Expression of Aquaporin 5 in the Late-Pregnant Rat Uterus

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

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