A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species

Zhou, Zengtong; Tang, Hongzhi; Wang, Weiwei; Zhang, Lige; Su, Fei; Wu, Yuanting; Bai, Linquan; Li, Sicong; Sun, Yuhui; Tao, Fei; Xu, Ping

doi:10.1038/s41421-021-00246-5

Cited by 21 publications

(12 citation statements)

References 50 publications

(53 reference statements)

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“…For mutants over-expressing candidate genes using shuttle vector pEZ15Asp, recombinant strains of ZM4 (pEZ-0994) and ZM4 (pEZ_cspL) grew significantly better than the control strain ZM4 (pEZ_Ep) (Fig. 6 e–h), which was consistent with previous reports that the overexpression of gene ZMO0994 and heterologous gene cspL can help promote cellular growth significantly at a high temperature [ 26 , 40 ]. In addition, when only groESL operon was overexpressed, the specific growth rate of recombinant strain ZM4 (pEZ_groESL) was significantly higher than that of ZM4 (pEZ_Ep) at high temperatures (Fig.…”

Section: Resultssupporting

confidence: 90%

“…However, this impact of overexpression of ZMO0994 gene on heat tolerance in Z. mobilis has not been explored yet. In addition to the chaperones discussed above, cold shock protein CspL from the thermophilic bacterium Bacillus coagulans 2–6 was systematically investigated recently, and the results demonstrated that CspL plays a role in heat tolerance, which can promote the growth of diverse microorganisms under heat stress including E. coli DH5α, S. cerevisiae INVSc1, and P. putida KT2440 through binding diverse RNA species at high temperatures [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

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Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Shen

Yang

et al. 2021

Biotechnol Biofuels

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The model ethanologenic bacterium Zymomonas mobilis has many advantages for diverse biochemical production. Although the impact of temperature especially high temperature on the growth and ethanol production of Z. mobilis has been reported, the transcriptional profiles of Z. mobilis grown at different temperatures have not been systematically investigated. In this study, Z. mobilis wild-type strain ZM4 was used to study the effect of a broad range of temperatures of 24, 30, 36, 40, and 45 °C on cell growth and morphology, glucose utilization and ethanol production, as well as the corresponding global gene expression profiles using RNA-Seq-based transcriptomics. In addition, a recombinant Z. mobilis strain expressing reporter gene EGFP (ZM4_EGFP) was constructed to study the effect of temperature on heterologous protein expression at different temperatures. Our result demonstrated that the effect of temperature on the growth and morphology of ZM4 and ZM4_EGFP were similar. The biomass of these two strains decreased along with the temperature increase, and an optimal temperature range is needed for efficient glucose utilization and ethanol production. Temperatures lower or higher than normal temperature investigated in this work was not favorable for the glucose utilization and ethanol production as well as the expression of exogenous protein EGFP based on the results of flow cytometry and Western blot. Temperature also affected the transcriptional profiles of Z. mobilis especially under high temperature. Compared with ZM4 cultured at 30 °C, 478 genes were up-regulated and 481 genes were down-regulated at 45 °C. The number of differentially expressed genes of ZM4 cultured at other temperatures (24, 36 or 40 °C) was relatively small though compared with those at 30 °C. Since temperature usually increases during the fermentation process, and heat tolerance is one of the important robustness traits of industrial strains, candidate genes related to heat resistance based on our RNA-Seq result and literature report were then selected for genetics study using the strategies of plasmid overexpression of candidate gene or replacement of the native promoter of candidate gene by an inducible Ptet promoter. The genetics studies indicated that ZMO0236, ZMO1335, ZMO0994, operon groESL, and cspL, which encodes Mrp family chromosome partitioning ATPase, flavoprotein WrbA, an uncharacterized protein, chaperonin Cpn10 and GroEL, and an exogenous cold shock protein, respectively, were associated with heat tolerance, and recombinant strains over-expressing these genes can improve their heat tolerance. Our work thus not only explored the effects of temperature on the expression of exogenous gene EGFP and endogenous genes, but also selected and confirmed several genes associated with heat tolerance in Z. mobilis, which provided a guidance on identifying candidate genes associated with phenotypic improvement through systems biology strategy and genetics studies for other microorganisms.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Shen

Yang

et al. 2021

Biotechnol Biofuels

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“…As mentioned above, due to the enormous interest of LAB, not only as probiotics per se, but also as starter microorganisms in the production of fermented foods, their ability to resist and cope with temperature changes is a feature of the utmost technological importance, given the different temperatures they will undergo during the processing and subsequent refrigeration or frozen storage [ 53 , 54 ]. This temperature tolerance becomes even more significant when talking about probiotics for veterinary use as feed supplements and, within this sector, aquaculture is the most challenging market, due to the extreme temperatures that are reached during the processing of fish feed.…”

Section: Resultsmentioning

confidence: 99%

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

et al. 2022

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Probiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.

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“…In our previous study, liver samples collected at 3 days postmortem could meet the requirements of next-generation sequencing (Wang et al, 2020). Because temperature is one of the most important environmental factors affecting the succession of microbes (Zhou et al, 2021), a reasonable explanation for the discrepancy between the previous study and the current study is that the ambient temperature in the present study (5 to 10°C) was substantially lower than that in the previous study (15 to 25°C), resulting in limited growth of microorganisms. This result suggests that in follow-up translational studies, the concept of accumulated degree-days, which integrates postmortem interval and ambient temperature (Heaton et al, 2010), should be used to minimize error caused by temperature fluctuation.…”

Section: Discussionmentioning

confidence: 99%

Microbial communities in the liver and brain are informative for postmortem submersion interval estimation in the late phase of decomposition: A study in mouse cadavers recovered from freshwater

et al. 2022

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IntroductionBodies recovered from water, especially in the late phase of decomposition, pose difficulties to the investigating authorities. Various methods have been proposed for postmortem submersion interval (PMSI) estimation and drowning identification, but some limitations remain. Many recent studies have proved the value of microbiota succession in viscera for postmortem interval estimation. Nevertheless, the visceral microbiota succession and its application for PMSI estimation and drowning identification require further investigation.MethodsIn the current study, mouse drowning and CO2 asphyxia models were developed, and cadavers were immersed in freshwater for 0 to 14 days. Microbial communities in the liver and brain were characterized via 16S rDNA high-throughput sequencing.ResultsOnly livers and brains collected from 5 to 14 days postmortem were qualified for sequencing. There was significant variation between microbiota from liver and brain. Differences in microbiota between the cadavers of mice that had drowned and those only subjected to postmortem submersion decreased over the PMSI. Significant successions in microbial communities were observed among the different subgroups within the late phase of the PMSI in livers and brains. Eighteen taxa in the liver which were mainly related to Clostridium_sensu_stricto and Aeromonas, and 26 taxa in the brain which were mainly belonged to Clostridium_sensu_stricto, Acetobacteroides, and Limnochorda, were selected as potential biomarkers for PMSI estimation based on a random forest algorithm. The PMSI estimation models established yielded accurate prediction results with mean absolute errors ± the standard error of 1.282 ± 0.189 d for the liver and 0.989 ± 0.237 d for the brain.ConclusionsThe present study provides novel information on visceral postmortem microbiota succession in corpses submerged in freshwater which sheds new light on PMSI estimation based on the liver and brain in forensic practice.

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A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species

Cited by 21 publications

References 50 publications

Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

Microbial communities in the liver and brain are informative for postmortem submersion interval estimation in the late phase of decomposition: A study in mouse cadavers recovered from freshwater

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