A cold‐induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro

Xie, Guosheng; Kato, Hideki; Sasaki, Koichi; Imai, Ryozo

doi:10.1016/j.febslet.2009.07.057

Cited by 79 publications

(50 citation statements)

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“…It has been demonstrated that ferritin could reduce accumulation of ROS in response to oxidant challenge (Orino et al 2001). OsTrx23, one of the cold-induced Trx h proteins, plays important roles in response to oxidative stress imposed by cold (Nuruzzaman et al 2008;Xie et al 2009). OsLti6, a hydrophobic transmembrane protein with low molecular weight, is involved in preserving plasma membrane integrity during cold stress in rice (Kim et al 2007;Morsy et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings

et al. 2010

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Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species (ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance.

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Section: Discussionmentioning

confidence: 99%

Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings

et al. 2010

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“…The effects of low temperatures on metabolism, physiology and gene expression of rice cultivars from the japonica subspecies have been shown (Xie et al 2009;Saad et al 2012). Although cold stress has an important impact on indica rice production in sub-tropical areas, reports about cold responses in different indica genotypes are rare (Zhang et al 2012a).…”

Section: Introductionmentioning

confidence: 99%

Identification and physiological characterization of two sister lines of indica rice (Oryza sativa L.) with contrasting levels of cold tolerance

et al. 2016

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Exposure to low temperature during germination and vegetative growth is a limiting factor to the establishment and development of rice seedlings. Higher cold tolerance of japonica than indica subspecies is well documented. However, reports of cold tolerance in indica genotypes are rare. We screened a large number of indica rice genotypes for cold tolerance during germination and initial vegetative growth. The indica genotypes IRGA 959-1-2-2F-4-1-4-A and IRGA 959-1-2-2F-4-1-4-D-1-CA-1, derived from the same cross, were characterized, respectively, as tolerant and sensitive to low temperature. Indexes of photosynthetic performance during light absorption were heavily affected by cold in both genotypes, but recovered after cold exposure only in the tolerant genotype. Activities of the antioxidant enzymes SOD and CAT (at the vegetative stage) and CAT and APX (at the germination stage) were higher in the tolerant than in the sensitive genotype. Expression of 20 genes previously related to cold response in rice was evaluated. Expression of OsLIP9 and OsWCOR413 were higher in the tolerant genotype upon or prior to cold exposure, respectively. The two sister lines show different molecular and physiological responses to low temperature stress. Further in-depth studies with these lines may help to identify new cold tolerance mechanisms in rice.Key words: abiotic stress, antioxidant enzymes, cold, gene expression, indica rice, JIP-test.Résumé : L'exposition à basse température pendant la germination et la croissance végétative est un facteur limitant à l'établissement et le développement des plantules de riz. La tolérance supérieure au froid observée en japonica par rapport à sous-espèces indica c'est bien documentée. Cependant, les rapports de la tolérance adéquate au froid dans les génotypes de indica sont encore rares. Cette étude a examiné plusieurs génotypes de indica en relation à leurs tolérances au froid pendant la germination et la croissance végétative initiale. Corresponding author: Janette P. Fett (email: jpfett@cbiot.ufrgs.br).*These authors contributed equally to this work.Abbreviations: ABS/RC, energy flux absorption per reaction center; APX, ascorbate peroxidase; CAT, catalase; DI 0 /RC, energy dissipation per reaction center; ET0/ABS, quantum yield of electron transport from QA − to the intersystem electron acceptors; ET0/CS, flow of electron transport per CS at t = 0; ET 0 /RC, electron transport flux per reaction center; ET 0 /TR 0 , probability that a trapped exciton moves an electron into the electron transport chain beyond QA − , at t = 0; H2O2, hydrogen peroxide; PIABS, performance index (potential) for energy conservation from photons absorbed by PSII to the reduction of intersystem electron acceptors; PIABS,total, performance index (potential) for energy conservation from photons absorbed by PSII to the reduction of PSI end acceptors; ROS, reactive oxygen species; SOD, superoxide dismutase; TBARS, thiobarbituric acid reactant substances; TR0/CS, flow of energy captured by CS at t = 0; TR0...

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“…OsTRXh1 is reportedly expressed in companion cells within the mature phloem (Ishiwatari et al, 1995(Ishiwatari et al, , 1998. Recently, another report showed that OsTRXh1 was induced by cold stress and negatively regulated the kinase activities of Oryza sativa Mitogen-activated Protein Kinase3 (OsMPK3) and OsMPK6 in vitro through a redox-dependent mechanism (Xie et al, 2009).…”

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confidence: 99%

An Apoplastic H-Type Thioredoxin Is Involved in the Stress Response through Regulation of the Apoplastic Reactive Oxygen Species in Rice

et al. 2011

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Thioredoxins (Trxs) are a multigenic family of proteins in plants that play a critical role in redox balance regulation through thiol-disulfide exchange reactions. There are 10 members of the h-type Trxs in rice (Oryza sativa), and none of them has been clearly characterized. Here, we demonstrate that OsTRXh1, a subgroup I h-type Trx in rice, possesses reduction activity in vitro and complements the hydrogen peroxide sensitivity of Trx-deficient yeast mutants. OsTRXh1 is ubiquitously expressed in rice, and its expression is induced by salt and abscisic acid treatments. Intriguingly, OsTRXh1 is secreted into the extracellular space, and salt stress in the apoplast of rice induces its expression at the protein level. The knockdown of OsTRXh1 results in dwarf plants with fewer tillers, whereas the overexpression of OsTRXh1 leads to a salt-sensitive phenotype in rice. In addition, both the knockdown and overexpression of OsTRXh1 decrease abscisic acid sensitivity during seed germination and seedling growth. We also analyzed the levels of hydrogen peroxide produced in transgenic plants, and the results show that more hydrogen peroxide is produced in the extracellular space of OsTRXh1 knockdown plants than in wild-type plants, whereas the OsTRXh1 overexpression plants produce less hydrogen peroxide under salt stress. These results show that OsTRXh1 regulates the redox state of the apoplast and influences plant development and stress responses.

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A cold‐induced thioredoxin h of rice, OsTrx23, negatively regulates kinase activities of OsMPK3 and OsMPK6 in vitro

Cited by 79 publications

References 32 publications

Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings

Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings

Identification and physiological characterization of two sister lines of indica rice (Oryza sativa L.) with contrasting levels of cold tolerance

An Apoplastic H-Type Thioredoxin Is Involved in the Stress Response through Regulation of the Apoplastic Reactive Oxygen Species in Rice

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