A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection

Tran, Dung Thi; Cho, Seungchan; Hoang, Phuong Mai; Kim, Jaedeok; Kil, Eui-Joon; Lee, Taek-Kyun; Rhee, Yong; Lee, Sukchan

doi:10.1007/s11105-015-0915-5

Cited by 12 publications

(4 citation statements)

References 27 publications

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“…When CSVd was inoculated into transgenic chrysanthemums expressing the conventional 3D8 scFv or codon-optimized for chrysanthemum, no disease symptoms were observed in either case, and all transgenic lines showed no disease symptoms. Additionally, CSVd was detected by RT-PCR in the inoculated leaves in all the lines produced, but was hardly in the newly developed upper leaves in almost all lines (Tran et al, 2016). This indicates that 3D8 scFv expression in plants can confer resistance not only to a wide range of viruses but also to viroids.…”

Section: -3-1-2 Nucleic Acid Antibodymentioning

confidence: 95%

Resistance and Tolerance to Viroid Infection

Nakada¹,

Li²,

Shimmen³

2022

Preprint

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Viroids are known the smallest plant pathogens, and although their genome sequences do not encode proteins, they can cause disease in economically important crops. In order to control viroid diseases and mitigate their damage, genetic resources used for breeding of the viroid-resistant crop have been searched, but the practical resistant trait has not been found in almost all viroid-crop combinations, as well as the tolerant trait. Due to the difficulty in exploiting naturally occurring resistance or tolerance, various effective strategies have been devised to control viroid diseases using non-transforming or transforming techniques. Meanwhile, extensive findings related to viroid resistance and tolerance may lead to confer resistance or tolerance to viroid infection by combining with the recently emerged new technologies (e.g., spray-induced gene silencing and genome-editing technologies), which are believed to be more environmentally viable and acceptable to the general public than previously reported approaches. In particular, some genome-modified crops produced by the latter technology are already on the market. In this review, we comprehensively summarize the current status about investigation of naturally occurring genetic traits for viroid resistance and tolerance, accumulating knowledge about host factors involved in viroid pathogenicity, and various basic technologies developed to try to possible viroid disease control strategies. Furthermore, we discuss prospects and challenges for the achievement of more effective, practical, and sustainable disease control of viroid.

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Section: -3-1-2 Nucleic Acid Antibodymentioning

confidence: 95%

Resistance and Tolerance to Viroid Infection

Nakada¹,

Li²,

Shimmen³

2022

Preprint

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“…The continuous innovation of sequencing technology provides great convenience for finding target genes, and various resistant chrysanthemum varieties have emerged in an endless stream [ 166 , 167 , 168 ]. Currently, transgenic methods for antiviral chrysanthemums have been reported [ 169 , 170 ], and Torata et al also cross-bred antiviral varieties to obtain new resistant varieties [ 171 , 172 ]. These findings provide a new direction for the much-needed study of nontoxic chrysanthemums.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Gao,

Chen,

Pan

et al. 2023

Viruses

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Chrysanthemum is an important functional plant that is used for food, medicine and tea. Functional chrysanthemums become infected with viruses all around the world, seriously lowering their quality and yield. Viral infection has become an important limiting factor in chrysanthemum production. Functional chrysanthemum is often propagated asexually by cutting during production, and viral infection of seedlings is becoming increasingly serious. Chrysanthemums can be infected by a variety of viruses causing different symptoms. With the development of biotechnology, virus detection and virus-free technologies for chrysanthemum seedlings are becoming increasingly effective. In this study, the common virus species, virus detection methods and virus-free technology of chrysanthemum infection are reviewed to provide a theoretical basis for virus prevention, treatment and elimination in functional chrysanthemum.

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“…Apart from strategies employing plant and pathogen genes, some researchers have tried using animal immunity genes to stimulate resistance in plants Tran et al, 2016). Plants engineered to express antibodies, referred to as plantibodies, may provide protection against pathogens.…”

Section: Transgenic Technology For Rust Resistance Breedingmentioning

confidence: 99%

Molecular breeding technologies and strategies for rust resistance in wheat (Triticum aestivum) for sustained food security

et al. 2017

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Wheat is an important cereal food crop providing key nutrients to humankind. Rusts are the most destructive pathogens of cereal crops, with the exception of rice, across the world and resistant cultivars have been widely employed to reduce the yield losses caused by them. The modern intensive monoculture of cultivars and changing climatic conditions has created congenial conditions for the emergence of new virulent races such as Ug99, which is a great concern for world food security. Conventional breeding efforts have not been effective in quickly developing new varieties with durable and broad‐spectrum resistance against the rapidly evolving rust pathogen races. However, in the last two decades, biotechnological methods such as marker‐assisted selection (MAS) and transgenic technology have provided novel strategies for enhancing resistance levels and durability in crop plants in a short span of time. Nevertheless, broad application of transgenics in agriculture is hindered by biosafety apprehensions. In recent years, improved versions of biotechnological breeding methods such as genomic selection, genome editing technologies, cisgenesis and intragenesis, RNA‐dependent DNA methylation (RdDM), agroinfiltration and reverse breeding are gaining popularity. These technologies provide a tremendous capability to manipulate crop plants more precisely than before and accelerate crop improvement efforts for sustained food production as well as overcoming safety concerns associated with food crops.

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A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection

Cited by 12 publications

References 27 publications

Resistance and Tolerance to Viroid Infection

Resistance and Tolerance to Viroid Infection

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Molecular breeding technologies and strategies for rust resistance in wheat (Triticum aestivum) for sustained food security

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