A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase

Patzoldt, William L.; Hager, Aaron G.; McCormick, Joel S.; Tranel, Patrick J.

doi:10.1073/pnas.0603137103

Cited by 186 publications

(255 citation statements)

References 31 publications

Supporting

Mentioning

241

Contrasting

Unclassified

Order By: Relevance

“…Results similar to those obtained with E. heterophylla were observed for Amaranthus tuberculatus (Patzoldt et al, 2006) and Ambrosia artemissiifolia (Rousonelos et al, 2012) resistant to PROTOX inhibiting herbicides, wherein the resistance is conditioned by a single dominant nuclear gene, but with incomplete dominance.…”

Section: Resultssupporting

confidence: 78%

“…The mechanism of resistance to PROTOX inhibitors was first elucidated in biotypes of A. tuberculatus, wherein a change in the amino acid sequence of the PROTOX enzyme determined the resistance, and a single nuclear gene with incomplete dominance was responsible for the mutation of the enzyme (Lee et al, 2008;Patzoldt et al, 2006). The discovery of the mechanism of resistance to PROTOX inhibiting herbicides in Ambrosia artemisiifolia biotypes biotypes is the second case of resistance elucidated, in which a mutation in the PROTOX enzyme, with through the substitution of an arginine for a leucine at position 98 confers resistance conditioned by a single nuclear gene with incomplete dominance (Rousonelos et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inheritance of Resistance to Protoporphyrinogen Oxidase Inhibitor Herbicides in Wild Poinsettia

et al. 2016

View full text Add to dashboard Cite

-Weed resistance to herbicides is a major global problem for agriculture. In recent years, the increased use of herbicides, without proper planning, has led to a large increase in the number of cases of weed biotypes that are resistant to one or more herbicide mechanism of action. Wild poinsettia biotypes (Euphorbia heterophylla), discovered in the State of Paraná, with resistance to herbicides that inhibit protoporphyrinogen oxidase (PROTOX) and acetolactate synthase (ALS), are the first case to exhibit multiple resistance in Brazil. This study analyzed the genetic inheritance of PROTOX inhibiting herbicide resistance in E. heterophylla. Crosses were conducted between biotypes that were susceptible (female parent) and those carrying multiple resistance to ALS and PROTOX inhibitors (male parent) to obtain the F 1 generation. Backcrosses were performed (RC 1 and RC 2 ) and the F 1 generation was advanced to F 2 . F 1 , F 2 , RC 1 , RC 2 generation plants and their parents were subjected to applications of the herbicide fomesafen (250 g i.a. ha -1 ). The observed frequencies in the F 2 generation were not different from the expected frequency of 3:1. All RC 2 individuals were resistant, while the RC 1 population showed a 1:1 segregation, which would be expected when a single gene controls the trait. Thus, it can be inferred that E. heterophylla resistance to PROTOX-inhibiting herbicides is conditioned by a single and dominant nuclear gene.

show abstract

Section: Resultssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Inheritance of Resistance to Protoporphyrinogen Oxidase Inhibitor Herbicides in Wild Poinsettia

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These mutations are typically nucleotide substitutions (Tranel & Wright, 2002), but there are one report of deletion of one entire codon of amino acids (Patzoldt et al, 2006).…”

Section: Target-site Mutationsmentioning

confidence: 99%

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

Roso

Vidal

2010

Planta daninha

View full text Add to dashboard Cite

-Glyphosate is an herbicide that inhibits the enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPs) (EC 2.5.1.19). EPSPs is the sixth enzyme of the shikimate pathway, by which plants synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan and many compounds used in secondary metabolism pathways. About fifteen years ago it was hypothesized that it was unlikely weeds would evolve resistance to this herbicide because of the limited degree of glyphosate metabolism observed in plants, the low resistance level attained to EPSPs gene overexpression, and because of the lower fitness in plants with an altered EPSPs enzyme. However, today 20 weed species have been described with glyphosate resistant biotypes that are found in all five continents of the world and exploit several different resistant mechanisms. The survival and adaptation of these glyphosate resistant weeds are related toresistance mechanisms that occur in plants selected through the intense selection pressure from repeated and exclusive use of glyphosate as the only control measure. In this paper the physiological, biochemical, and genetic basis of glyphosate resistance mechanisms in weed species are reviewed and a novel and innovative theory that integrates all the mechanisms of non-target site glyphosate resistance in plants is presented.Keywords: membrane carrier proteins, herbicide, weed resistance.RESUMO -Glyphosate é uma glicina fosfonada e inibe a enzima 5-enolpiruvil-shikimato-3-fosfato sintase (EPSPS) (EC 2.5.1.19). EPSPS é a sexta enzima da rota do shikimato, na qual são sintetizados os compostos do metabolismo secundário e os aminoácidos aromáticos fenilalanina, tirosina e triptofano. Alguns autores hipotetizaram que seria improvável a evolução de plantas daninhas resistentes a este herbicida. As justificativas estariam relacionadas à limitada metabolização de glyphosate nas plantas, ao baixo nível de resistência obtido com a superexpressão do gene EPSPS e à alta penalidade de adaptação oriunda de mutações no gene EPSPS. Mas, atualmente estão descritas 18 espécies com biótipos resistentes distribuídas em 17 países. A sobrevivência e adaptação estão relacionadas aos mecanismos de resistência que ocorrem nas plantas daninhas selecionadas em função da intensa pressão de seleção do herbicida. Esta revisão objetiva apresentar e discutir evidências fisiológicas, bioquímicas e genéticas que fundamentam os principais mecanismos envolvidos na resistência ao herbicida glyphosate em plantas daninhas.Palavras-chave: herbicida, proteínas carregadoras de membrana, planta daninha.

show abstract

“…In some cases, even more facile transgenic systems that rely on nonplant organisms (such as bacteria or yeast) have been utilized. For example, Baerson et al (2002) and Patzoldt et al (2006) used Escherichia coli mutants (which were deficient for EPSPS and PPO, respectively) to confirm target-site mutations suspected of conferring resistance to either glyphosate or PPO inhibitors.…”

Section: Quick Assays For Testing Resistancementioning

confidence: 99%

“…Resistance to PPO-inhibiting herbicides in waterhemp was conferred not by an SNP in the target-site gene, but rather by a codon deletion (Patzoldt et al 2006). A PASA marker was developed for the detection of this mutation (Lee et al 2008).…”

Section: Quick Assays For Testing Resistancementioning

confidence: 99%

Rapid Assay Evaluation of Plant Response to Protoporphyrinogen Oxidase (Protox)-Inhibiting Herbicides

2006

View full text Add to dashboard Cite

Protoporphyrinogen oxidase (protox)-inhibiting herbicides damage cell membranes, resulting in electrolyte leakage. A whole-plant dose-response study and a rapid assay that measured electrolyte leakage was used to determine the response of wild mustard, soybean, and protox inhibitor–susceptible and protox inhibitor–resistant common waterhemp to increasing doses of three protox inhibitors: acifluorfen, fomesafen, and sulfentrazone. For the dose-response study, whole plants were treated with the three protox-inhibitor herbicides. Electroconductivity assay 1 consisted of cutting discs from leaf tissue and submerging them in an incubation medium containing concentrations of acifluorfen, fomesafen, or sulfentrazone. In electroconductivity assay 2, the entire leaf was treated with solutions containing acifluorfen, fomesafen, or sulfentrazone. The whole-plant dose-response study showed increasing visible injury with increasing herbicide rates for all species and all herbicides. The order of visible injury was wild mustard > susceptible common waterhemp > resistant common waterhemp > soybean. In assay 1, electrolyte leakage from leaf discs treated with acifluorfen or fomesafen increased with increasing herbicide concentrations, and was similar for all species. In contrast, electrolyte leakage from leaf discs treated with sulfentrazone did not increase with increasing herbicide concentrations for any species. In assay 2, only wild mustard leaf discs increased in electrolyte leakage with increasing herbicide rates of acifluorfen, fomesafen, and sulfentrazone and followed the regression curves established by the whole-plant dose-response study. However, assay 2 was not able to distinguish between susceptible wild mustard and tolerant soybean, or between susceptible and resistant waterhemp.

show abstract

A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase

Cited by 186 publications

References 31 publications

Inheritance of Resistance to Protoporphyrinogen Oxidase Inhibitor Herbicides in Wild Poinsettia

Inheritance of Resistance to Protoporphyrinogen Oxidase Inhibitor Herbicides in Wild Poinsettia

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

Rapid Assay Evaluation of Plant Response to Protoporphyrinogen Oxidase (Protox)-Inhibiting Herbicides

Contact Info

Product

Resources

About