A clinical case–control study on the association between mannose‐binding lectin and susceptibility to HIV‐1 infection among northern Han Chinese population

Abstract

Mannose-binding lectin (MBL) is a key molecule of the innate immune system and a competent to bind carbohydrates of a variety of microorganisms, resulting in complement activation and opsonophagocytosis against various pathogens. However, there is no systemic investigation on the MBL's role in innate immune responses against HIV-1 infection among northern Han Chinese. This study investigated the association between MBL and HIV-1 infection susceptibility among northern Han Chinese. A total of 91 HIV-1 infected patients and 91 HIV-1 seronegative healthy individuals were recruited. Six polymorphisms of MBL2 gene were genotyped by pyrosequencing. The quantitative measurement of serum MBL concentration and MBL complex activity were performed by ELISA. The CD4+ T-cell counts were determined by flow cytometry. The plasma viral loads of 91 HIV-1 infected patients were determined by bDNA method. The results show that there is an association between MBL and HIV-1 infection susceptibility among northern Han Chinese. The individuals with B variant, low serum MBL concentration and low MBL complex activity are more susceptible to HIV-1 infection.

Role of Mannose-Binding Lectin Deficiency in HIV-1 and Schistosoma Infections in a Rural Adult Population in Zimbabwe

“…We found no association between presence of MBL2 structural variants and promoter variants with HIV infection, in accordance with other findings [ 38 , 90 ], in contrast to reports of significant associations [ 37 , 83 , 84 , 91 ]. Similar results to ours of no association between MBL2 polymorphism and HIV infection, reported in a Colombian population, were explained as being due to existence of redundant constitutive acquired immune defence systems that can complement or take up the innate defence functions provided by MBL, which are likely favoured under conditions of high pathogen exposure [ 38 ].…”

“…The results of our study showed no difference in plasma MBL concentration between the HIV positive and HIV negative individuals, consistent with other reports [ 80 – 82 ]. In contrast, some have reported protective effect of normal plasma MBL levels and increased susceptibility due to low MBL levels [ 30 , 37 , 83 , 84 ], but some reported a deleterious effect of normal plasma MBL levels where high MBL levels were associated with acquiring HIV infection [ 39 ].…”

“…The reason for the differences in plasma MBL levels, MBL2 genotypes and HIV association outcomes is not clear [ 54 , 55 , 96 ] but this maybe because studies have been carried out in different populations, homosexual groups [ 37 ] and some in heterosexuals [ 30 , 38 , 91 ] and also the methods employed in these studies are different with some measuring plasma MBL levels only [ 30 , 39 , 81 , 82 , 97 , 98 ], others assessing structural alleles only [ 38 , 91 , 99 ], some looked at both [ 37 , 80 , 83 , 84 , 90 ] others in addition looked at MBL2 promoter region alleles [ 92 , 100 ]. Our study investigated all three MBL parameters, plasma MBL levels, polymorphism in the MBL2 exon 1 gene and promoter region variants.…”

Complement and HIV-I infection/HIV-associated neurocognitive disorders

“…Three genetic studies of Chinese populations have demonstrated that MBL deficiencies are associated with increased susceptibility to HIV-1 infection and disease progression (Sheng et al, 2010) (Tan et al, 2009) (Li et al, 2013) and serum circulating levels of normal MBL in HIV-1-infected patients could be an important auxiliary biological marker in association with CD4(+) T cell counts in the evaluation of HIV-1 disease progression (Tan et al, 2009). These findings are consistent with studies previously conducted by Dzwonek et al (2006) in the UK (Dzwonek et al, 2006).…”

Μελέτη των απλότυπων της λεκτίνης που δεσμεύει τη μαννόζη (mannose binding lectin MBL) στον ελληνικό πληθυσμό και σε ασθενείς με λοιμώξεις

“…Η ίδια ομάδα ερευνητών βρήκε σημαντικά χαμηλότερα επίπεδα MBL σε ασθενείς με HIV λοίμωξη σε σχέση με τους μάρτυρες στην υπο-Σαχάριο Αφρική408 . Επόμενες μελέτες σε άλλους πληθυσμούς έδειξαν παρόμοια αποτελέσματα[409][410][411][412] . Μία τουλάχιστον μελέτη δεν βρήκε καμία συσχέτιση413 ενώ μια μεγάλη έρευνα ασθενών μαρτύρων 414 έδειξε αντίστροφα ευρήματα.…”