A circulating non-coding RNA panel as an early detection predictor of non-small cell lung cancer

Peng, Hua; Wang, Jia; Li, Jia; Zhao, Mei; Huang, Shiang Suo; Gu, Yating; Li, Yan; Sun, Xiufa; Yang, Lin; Luo, Qin; Huang, Changzhi

doi:10.1016/j.lfs.2016.03.002

Cited by 62 publications

(45 citation statements)

References 18 publications

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“…Previous studies have reported that miR-1254 expression is dysregulated in human breast cancer[51], retinoblastoma[52] and NSCLC[53, 54]. MiR-1254 was identified as a circulating miRNA downregulated in NSCLC patient serum, compared with healthy control [54].…”

Section: Discussionmentioning

confidence: 99%

MiR-1254 suppresses HO-1 expression through seed region-dependent silencing and non-seed interaction with TFAP2A transcript to attenuate NSCLC growth

et al. 2017

PLoS Genet

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MicroRNAs (miRNAs) are a class of small non-coding RNAs, which direct post-transcriptional gene silencing (PTGS) and function in a vast range of biological events including cancer development. Most miRNAs pair to the target sites through seed region near the 5’ end, leading to mRNA cleavage and/or translation repression. Here, we demonstrated a miRNA-induced dual regulation of heme oxygenase-1 (HO-1) via seed region and non-seed region, consequently inhibited tumor growth of NSCLC. We identified miR-1254 as a negative regulator inhibiting HO-1 translation by directly targeting HO-1 3’UTR via its seed region, and suppressing HO-1 transcription via non-seed region-dependent inhibition of transcriptional factor AP-2 alpha (TFAP2A), a transcriptional activator of HO-1. MiR-1254 induced cell apoptosis and cell cycle arrest in human non-small cell lung carcinoma (NSCLC) cells by inhibiting the expression of HO-1, consequently suppressed NSCLC cell growth. Consistently with the in vitro studies, mouse xenograft studies validated that miR-1254 suppressed NSCLC tumor growth in vivo. Moreover, we found that HO-1 expression was inversely correlated with miR-1254 level in human NSCLC tumor samples and cell lines. Overall, these findings identify the dual inhibition of HO-1 by miR-1254 as a novel functional mechanism of miRNA, which results in a more effective inhibition of oncogenic mRNA, and leads to a tumor suppressive effect.

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Section: Discussionmentioning

confidence: 99%

MiR-1254 suppresses HO-1 expression through seed region-dependent silencing and non-seed interaction with TFAP2A transcript to attenuate NSCLC growth

et al. 2017

PLoS Genet

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“…Notably, lncRNAs can serve as minimally invasive and sensitive molecular markers for the screening and early diagnosis of lung cancer [55]. Peng et al constructed a serum ncRNA panel ( miR-1254 , miR-485-5p, miR-574-5p , and lncRNA MALAT1 ), and tested whether the ncRNA panel could distinguish NSCLC patient samples from controls.…”

Section: Non-coding Rnamentioning

confidence: 99%

“…Peng et al constructed a serum ncRNA panel ( miR-1254 , miR-485-5p, miR-574-5p , and lncRNA MALAT1 ), and tested whether the ncRNA panel could distinguish NSCLC patient samples from controls. Their results indicated that the four ncRNA panel can serve as a convenient tool for early NSCLC diagnosis [55]. LncRNAs are stable even in body fluids and show tissue-specific expression.…”

Section: Non-coding Rnamentioning

confidence: 99%

Major Tumor Suppressor and Oncogenic Non-Coding RNAs: Clinical Relevance in Lung Cancer

Inamura

2017

Cells

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Lung cancer is the leading cause of cancer deaths worldwide, yet there remains a lack of specific and sensitive tools for early diagnosis and targeted therapies. High-throughput sequencing techniques revealed that non-coding RNAs (ncRNAs), e.g., microRNAs and long ncRNAs (lncRNAs), represent more than 80% of the transcribed human genome. Emerging evidence suggests that microRNAs and lncRNAs regulate target genes and play an important role in biological processes and signaling pathways in malignancies, including lung cancer. In lung cancer, several tumor suppressor/oncogenic microRNAs and lncRNAs function as biomarkers for metastasis and prognosis, and thus may serve as therapeutic tools. In this review, recent work on microRNAs and lncRNAs is introduced and briefly summarized with a focus on potential biological and therapeutic applications.

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“…To further support its potential role during cancer progression, MALAT1 has been shown to induce EMT and to regulate angiogenesis [52]. Its diagnostic efficiency as a cell-free serum/plasma molecule has been demonstrated in smallcell lung cancer (NSCLC) and pancreas cancer patients [53,54]. Another lncRNA that is often quantified in studies is HOX Transcript Antisense RNA (HOTAIR).…”

Section: Diagnostic Value Of Cell-free Lncrnas In Plasma and Serummentioning

confidence: 99%

Diagnostic relevance of circulating cell-free and exosomal microRNAs and long non-coding RNAs in blood of cancer patients

Schwarzenbach

2016

LaboratoriumsMedizin

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MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) belong to a large group of non-coding RNA (ncRNA) molecules that possess diverse functions in epigenetic and chromatin structure modifications. In particular, they post-transcriptionally inhibit the protein expression of their target mRNAs and therefore, are involved in different tumor-associated signaling pathways. As the expression of both ncRNAs is frequently dysregulated in cancer and shows unique signatures, they may play a crucial role in cancer pathogenesis. They are passively and actively released into the blood circulation, and accordingly, their plasma/serum and exosomal profiles reflect disease development, tumor load and malignant progression towards metastatic relapse. Considering the biology and nature of ncRNAs in the regulation of different cellular processes, they may have potential clinical utility as blood-based tumor markers and future therapeutic targets in the treatment of cancer patients. The present review focuses on the diagnostic value of cellfree and exosomal ncRNAs in plasma/serum of cancer patients and the challenges to establish them as future tumor markers.Keywords: cell-free miRNAs; cell-free lncRNAs; exosomes; liquid biopsy.Zusammenfassung: MikroRNAs (miRNAs) und lange nicht-kodierende RNAs (lncRNAs) gehören zu einer großen Gruppe von nicht-kodierenden RNA-(ncRNA) Molekülen, die Funktionen bei epigenetischen und Chromatinstruktur-Modifikationen besitzen. Insbesondere inhibiieren sie post-transkriptionell die Proteinexpression ihrer Ziel-RNAs und sind deshalb in verschiedene Tumorassozi ierte Signalwege involviert. Da die Expression der beiden ncRNAs häufig bei Krebs dereguliert ist und einzigartige Signaturen aufweist, spielen sie eine wichtige Rolle in der Pathogenese von Krebs. Sie werden passiv und aktiv in den Blutkreislauf befreit und folglich reflektieren ihre Plasma/Serum-und exosomale Profile die Entwicklung der Krankheit, die Tumorlast und die maligne Progression bis hin zum metastatischen Relaps. Betreffend der Biologie und Natur der ncRNAs in der Regulation der verschiedenen zellulären Prozesse, sind sie als Blut-basierte Marker und zukünftige therapeutische Zielemoleküle bei der Behandlung von Krebspatienten für die Klinik potenziell wichtig. Der vorliegende Reviewartikel befasst sich mit dem diagnostischen Wert von zellfreien und exosomalen ncRNAs im Plasma/Serum von Krebspatienten und den Herausforderungen sie als zukünftige Tumormarker zu etablieren.Schlüsselwörter: Exosomen; flüssige Biopsie; zellfreie miRNAs; zellfreie lncRNAs.

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A circulating non-coding RNA panel as an early detection predictor of non-small cell lung cancer

Cited by 62 publications

References 18 publications

MiR-1254 suppresses HO-1 expression through seed region-dependent silencing and non-seed interaction with TFAP2A transcript to attenuate NSCLC growth

MiR-1254 suppresses HO-1 expression through seed region-dependent silencing and non-seed interaction with TFAP2A transcript to attenuate NSCLC growth

Major Tumor Suppressor and Oncogenic Non-Coding RNAs: Clinical Relevance in Lung Cancer

Diagnostic relevance of circulating cell-free and exosomal microRNAs and long non-coding RNAs in blood of cancer patients

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