A Circulating Heat-Resistant Mucin-Like Antigen in Patients With Lung-Cancer Detected by a New Murine Monoclonal-Antibody

Kohno, Nobuoki; Miyake, Masayuki; Inoue, Yui; Yokoyama, A; Hiwada, Kunio; Tanabe, Masahiro; Yamakido, Michio; Kyoizumi, Seishi; Akiyama, Masachika

doi:10.3892/ijo.1.6.649

Cited by 2 publications

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“…6 Anti-KL6/MUC1 monoclonal antibody (anti-KL6 mAb) is a promising probe for monitoring KL6/MUC1 levels in bloodstream. 7 In 2009, we identified for the first time the minimal and essential epitope structure recognized by anti-KL6 mAb to be heptapeptide Pro-Asp-Thr-Arg-Pro-Ala-Pro having sialyl-T antigen [Neu5Acα(2→3)Galβ(1→3)GalNAcα1→] at the Thr residue. 8 It is important to note accumulated evidences that anti-KL6 mAb reacts often with serum MUC1 fragments in patients suffering from various cancers in addition to the ILDs.…”

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confidence: 99%

Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases

et al. 2016

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Synthetic macromolecular MUC1 glycopeptides have been used to unravel molecular mechanisms in antibody recognition of disease-specific epitopes. We have established a novel synthetic strategy for MUC1 tandem repeats having complex O-glycosylation states at each repeating unit based on convergent solid-phase fragment condensation under microwave irradiation. We have accomplished the synthesis of 77 amino acid MUC1 glycopeptides (MW = 12 759) having three major antigenic O-glycoforms [Tn, core 1 (T), and core 2 structures] at 10 designated positions out of 19 potential O-glycosylation sites. We demonstrate that the macromolecular MUC1 glycopeptide displaying the essential glycopeptidic neoepitope Pro-Asp-Thr(sialyl-T)-Arg-Pro-Ala-Pro at two different tandem repeats is an excellent serum MUC1 model showing ideal stoichiometric binding with anti-KL6/MUC1 antibody in the sandwich ELISA to quantify human serum KL6/MUC1 levels as a critical biomarker of interstitial lung diseases.

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confidence: 99%

Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases

et al. 2016

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A New Serum Tumor Marker, CAM 123‐6, Highly Specific to Pulmonary Adenocarcinoma

Hamada

Kohno

Hiwada

1994

Japanese Journal of Cancer Research

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KL‐6, a circulating mucin‐like glycoprotein, is a pulmonary adenocarcinoma‐associated antigen and is also regarded as an indicator of disease activity of interstitial pneumonitis. KL‐6 has extensive heterogeneous antigenic determinants and consists of multiple heterogeneous antigen molecules. We have searched for circulating KL‐6‐associated glycoproteins with superior diagnostic value to KL‐6 as a tumor marker for pulmonary adenocarcinoma. A new murine monoclonal antibody EH‐123 reacting with an asialosugar chain on KL‐6 was established. A new KL‐6‐associated molecule detected by a bimonoclonal bideterminant sandwich assay using the EH‐123 antibody as a catcher and horseradish peroxidase‐labeled KL‐6 as a tracer was designated as CAM 123‐6. In 59% (22 of 37) of patients with pulmonary adenocarcinoma, serum levels of CAM 123‐6 were abnormally elevated and the positive rate increased with the progression of clinical stage. Elevated levels were not detected in normal individuals or in patients with benign lung diseases, other histologic types of lung cancer, gastric cancer, colon cancer or breast cancer. CAM 123‐6 was more specific to pulmonary adenocarcinoma than carcinoembryonic antigen (CEA), but the sensitivity of CAM 123‐6 for pulmonary adenocarcinoma was similar to that of CEA. CAM 123‐6 is a promising candidate as a serum tumor marker for pulmonary adenocarcinoma.

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A Circulating Heat-Resistant Mucin-Like Antigen in Patients With Lung-Cancer Detected by a New Murine Monoclonal-Antibody

Cited by 2 publications

References 0 publications

Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases

Convergent Solid-Phase Synthesis of Macromolecular MUC1 Models Truly Mimicking Serum Glycoprotein Biomarkers of Interstitial Lung Diseases

A New Serum Tumor Marker, CAM 123‐6, Highly Specific to Pulmonary Adenocarcinoma

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