A Circular RNA–DNA Enzyme Obtained by in Vitro Selection

Kong, Xiangduo; Zhu, Shizhen; Gou, Xin; Wang, Xiaoping; Zhang, Hongying; Zhang, Jin

doi:10.1006/bbrc.2002.6767

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…The hammerhead ribozyme, composed of RNA, was found to be unstable and easily degradable, and additionally lacks a regulation domain, which normally facilitates the formation of complicated spatial structure with moderate rigidity and flexibility. To address the instability of the hammerhead ribozyme, we obtained a circular RNA-DNA enzyme by in vitro selection, which was composed of the hammerhead ribozyme as the active site, as well as backbone DNA with a regulating sequence [12]. Compared to the linear hammerhead ribozyme, the stability of the circular RNA-DNA enzyme was substantially enhanced.…”

Section: Introductionmentioning

confidence: 99%

Design and switch of catalytic activity with the DNAzyme–RNAzyme combination

et al. 2007

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Design and switch of catalytic activity in enzymology remains a subject of intense investigation. Here, we employ a DNAzyme-RNAzyme combination strategy for construction of a 10-23 deoxyribozyme-hammerhead ribozyme combination that targets different sites of the b-lactamase mRNA. The 10-23 deoxyribozyme-hammerhead ribozyme combination gene was cloned into phagemid vector pBlue-scriptIIKS (+). In vitro the single-strand recombinant phagemid vector containing the combination sequence exhibited 10-23 deoxyribozyme activity, and the linear transcript displayed hammerhead ribozyme activity. In bacteria, the 10-23 deoxyribozyme-hammerhead ribozyme combination inhibited the b-lactamase expression and repressed the growth of drug-resistant bacteria. Thus, we created a DNAzyme-RNAzyme combination strategy that provides a useful approach to design and switch of catalytic activities for nucleic acid enzymes.

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Section: Introductionmentioning

confidence: 99%