A circovirus and cycloviruses identified in feces of bobcats (Lynx rufus) in California

Cerna, Gabriella; Serieys, Laurel E. K.; Riley, Seth P. D.; Richet, Cécile; Kraberger, Simona; Varsani, Arvind

doi:10.1007/s00705-022-05656-8

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…The integrated themes were fed back to the research subjects for veri cation, and the themes were de ned through group discussions. In a mixed analysis, the results of the two separate analyses were discussed together and were rehearsed, which was consistent with the approach proposed by Creswell [20,21] . 4 Results…”

Section: Discussionsupporting

confidence: 73%

Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study

Yu¹,

Song²,

Pei³

et al. 2023

Preprint

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Background As the field of nursing science continues to evolve and higher education expands, enhancing empathy and humanistic care skills among nursing students prior to their clinical practice has become a crucial responsibility for nursing educators. To investigate the impact of the TPKCEE compound teaching model, which is based on narrative nursing, on the cultivation of empathy and humanistic care qualities among students in the ideological and political teaching of obstetrics and gynecology nursing. Methods A convergent mixed method design was used, which consisted of a quasi-experimental design and a semistructured interview.Data collection took place from September 2021 to February 2023. A convenience sampling method was used to select 61 fulltime third-year nursing students in a school in China (a control group of 27 and an experimental group of 34). The control group was exposed to traditional curriculum, while the experimental group used the TPCKEE teaching model based on narrative nursing. Jefferson Empathy Scale (JSE) and Caring Ability Scale (CAI) were used for quantitative assessment before and after the course, descriptive statistics and Mann-Whitney U test were used to compare the empathy and caring ability of the two groups of students, and semi-structured interviews were used for qualitative assessment. Thematic analysis was used to understand the development of students in humanistic care, empathy and learning experience. Results After teaching, the total score of empathy and the dimension of transposition, emotion and viewpoint, and the total score of CAI and the dimension of understanding and courage of nursing students in the control group were better than those in the control group (P < 0.05). Conclusion The TPCKEE teaching model based on narrative nursing effectively improves empathy, caring behavior, and classroom teaching outcomes in third-year nursing students.

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Section: Discussionsupporting

confidence: 73%

Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study

Yu¹,

Song²,

Pei³

et al. 2023

Preprint

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“…In our study, 1989 viral contigs from this phylum were identified across 9 families in these three WWTPs (Figure S2C ). Cressdnaviricota viruses are known for their diversity and presence in various habitats, including those associated with plants, animals, and humans [ 27 , 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

A panoramic view of the virosphere in three wastewater treatment plants by integrating viral‐like particle‐concentrated and traditional non‐concentrated metagenomic approaches

Zhang,

Tang,

Jin

et al. 2024

iMeta

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Wastewater biotreatment systems harbor a rich diversity of microorganisms, and the effectiveness of biotreatment systems largely depends on the activity of these microorganisms. Specifically, viruses play a crucial role in altering microbial behavior and metabolic processes throughout their infection phases, an aspect that has recently attracted considerable interest. Two metagenomic approaches, viral‐like particle‐concentrated (VPC, representing free viral‐like particles) and non‐concentrated (NC, representing the cellular fraction), were employed to assess their efficacy in revealing virome characteristics, including taxonomy, diversity, host interactions, lifestyle, dynamics, and functional genes across processing units of three wastewater treatment plants (WWTPs). Our findings indicate that each approach offers unique insights into the viral community and functional composition. Their combined use proved effective in elucidating WWTP viromes. We identified nearly 50,000 viral contigs, with Cressdnaviricota and Uroviricota being the predominant phyla in the VPC and NC fractions, respectively. Notably, two pathogenic viral families, Asfarviridae and Adenoviridae, were commonly found in these WWTPs. We also observed significant differences in the viromes of WWTPs processing different types of wastewater. Additionally, various phage‐derived auxiliary metabolic genes (AMGs) were active at the RNA level, contributing to the metabolism of the microbial community, particularly in carbon, sulfur, and phosphorus cycling. Moreover, we identified 29 virus‐carried antibiotic resistance genes (ARGs) with potential for host transfer, highlighting the role of viruses in spreading ARGs in the environment. Overall, this study provides a detailed and integrated view of the virosphere in three WWTPs through the application of VPC and NC metagenomic approaches. Our findings enhance the understanding of viral communities, offering valuable insights for optimizing the operation and regulation of wastewater treatment systems.

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“…Circoviruses have been identified in many terrestrial and aquatic mammals, including most families of the order Carnivora, such as ursids (Alex et al, 2020), viverrids (Nishizawa et al, 2018), mustelids (Lian et al, 2014; Bandoo et al, 2021), herpestids (Gainor et al, 2021), felids (Payne et al, 2020; Cerna et al, 2023), pinnipeds (Chiappetta et al, 2017; Patterson et al, 2021), and canids (Kapoor et al, 2012; Bexton et al, 2015). Although 60 circoviral species have currently been defined, the pathogenic role and ecology of these viruses, including their potential host ranges, have been only rarely investigated as most of them have been discovered within the framework of metagenomic investigations.…”

Section: Introductionmentioning

confidence: 99%

Diverse fox circovirus (Circovirus canine) variants circulate at high prevalence in grey wolves (Canis lupus) from the Northwest Territories, Canada

Canuti,

King,

Franzo

et al. 2024

Preprint

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Canine circovirus (CanineCV) has a worldwide distribution in dogs and is occasionally detected in wild carnivorans, indicating its ability for cross-species transmission. However, fox circovirus, a subclade of CanineCV, has been identified exclusively in wild canids. We analyzed spleen samples from 159 grey wolves from the Northwest Territories, Canada, to investigate the molecular epidemiology of CanineCV and formulate hypotheses about virus ecology and evolution. Overall, 72 (45.3%) animals tested positive. Virus prevalence was similar between males and females, adults and juveniles, and across the investigated years and locations. CanineCV infection was not associated with a poor body condition. While the percentage of co-infections with canine parvoviruses, investigated in a previous study, was high (63/72, 87.5%), the rate of parvovirus infection in CanineCV-negative animals was significantly lower (48.3%, p < 0.001) and CanineCV infection was associated with a 7.5- and 2.4-fold increase in the risk of acquiring canine parvovirus 2 or canine bufavirus infections, respectively (odds ratios: 3.5-16.9 and 1.3-5.8). Although common risk factors cannot be ruled out, this suggests that CanineCV may facilitate parvoviral super-infections. Sequencing revealed high genetic diversity, further exacerbated by recombination. Of the 69 sequenced strains, 87.5% were fox circoviruses, five were related to a fox circovirus-like recombinant strain, and one belonged to a distant clade. In the phylogenetic analysis, the viruses were distributed according to sampling locations, with some viruses being geographically restricted. Different clades of viruses were identified in the same areas and over multiple years (2007-2019), indicating the co-existence of multiple endemic lineages. Phylogenetic analysis of all available complete fox circovirus genomes (32 from foxes and 15 from wolves from North America and Europe) demonstrated four lineages, each including sequences from this study. Within each lineage, strains segregated geographically and not by host. This implies that, although multiple lineages co-exist, viruses do not frequently move between locations. Finally, viruses from Europe and North America were mixed, indicating that the origin of the four lineages might predate the segregation of European and American wolf and fox populations. Given the high prevalence and diversity of fox circoviruses in wolves, these animals should be considered reservoir hosts for these viruses. Although we cannot exclude a lower susceptibility of dogs, the lack of fox circovirus in dogs could be due to environmental circumstances that prevented its spread to dogs. Given the high diversity and wild host specificity, we presume a long-lasting association between fox circovirus and canine hosts and hypothesize a higher likelihood of transmission from dogs to wild animals than vice versa. Further studies should investigate other sympatric wild species and additional locations to explore the reasons behind the marked difference in cross-species transmission dynamics among the various clades.

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A circovirus and cycloviruses identified in feces of bobcats (Lynx rufus) in California

Cited by 10 publications

References 33 publications

Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study

Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study

A panoramic view of the virosphere in three wastewater treatment plants by integrating viral‐like particle‐concentrated and traditional non‐concentrated metagenomic approaches

Diverse fox circovirus (Circovirus canine) variants circulate at high prevalence in grey wolves (Canis lupus) from the Northwest Territories, Canada

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