A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress

Song, Aiping; Zhu, Xirong; Chen, Fadi; Gao, He; Jiang, Jiafu; Chen, Sumei

doi:10.3390/ijms15035063

Cited by 89 publications

(51 citation statements)

References 48 publications

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“…There were two transgenic lines to exclude the effect of the insertion site on the characteristics of the offspring. Additionally, there was no significant difference between the empty vector lines and wild plants before or after the salt treatment as descried previously Shan et al 2012;Song et al 2014b).…”

Section: Discussionmentioning

confidence: 60%

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Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Song

Gao

et al. 2015

Plant Cell Rep

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CmWRKY17 was induced by salinity in chrysanthemum, and it might negatively regulate salt stress in transgenic plants as a transcriptional repressor. WRKY transcription factors play roles as positive or negative regulators in response to various stresses in plants. In this study, CmWRKY17 was isolated from chrysanthemum (Chrysanthemum morifolium). The gene encodes a 227-amino acid protein and belongs to the group II WRKY family, but has an atypical WRKY domain with the sequence WKKYGEK. Our data indicated that CmWRKY17 was localized to the nucleus in onion epidermal cells. CmWRKY17 showed no transcriptional activation in yeast; furthermore, luminescence assay clearly suggested that CmWRKY17 functions as a transcriptional repressor. DNA-binding assay showed that CmWRKY17 can bind to W-box. The expression of CmWRKY17 was induced by salinity in chrysanthemum, and a higher expression level was observed in the stem and leaf compared with that in the root, disk florets, and ray florets. Overexpression of CmWRKY17 in chrysanthemum and Arabidopsis increased the sensitivity to salinity stress. The activities of superoxide dismutase and peroxidase and proline content in the leaf were significantly lower in transgenic chrysanthemum than those in the wild type under salinity stress, whereas electrical conductivity was increased in transgenic plants. Expression of the stress-related genes AtRD29, AtDREB2B, AtSOS1, AtSOS2, AtSOS3, and AtNHX1 was reduced in the CmWRKY17 transgenic Arabidopsis compared with that in the wild-type Col-0. Collectively, these data suggest that CmWRKY17 may increase the salinity sensitivity in plants as a transcriptional repressor.

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Section: Discussionmentioning

confidence: 60%

“…Leaf disks (5 mm diameter) taken from young leaves of 'Jinba' cultured in vitro were used as explants (Song et al 2014b). The transformants were selected on culture medium containing 8 mg/L hygromycin.…”

Section: Plasmid Construction and Transformation Of Chrysanthemum Andmentioning

confidence: 99%

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Song

Gao

et al. 2015

Plant Cell Rep

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“…The Plant Small RNA Target Analysis Server (psRNA Target: http://plantgrn.noble.org/psRNATarget) was used to identify the miRNA target genes in the PtrDof families with default parameters. FirstChoice RLM-RACE Kit (Invitrogen, Thermo Fisher Scientific) was used to perform RLM-RACE and illustrate the predicted targets following the methods researched by Song et al [33]. The gene-specific primers for RLM- RACE are presented in S2 Table.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Dof Transcription Factors and Their Responses to Osmotic Stress in Poplar (Populus trichocarpa)

et al. 2017

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The DNA-binding One Zinc Finger (Dof) genes are ubiquitous in many plant species and are especial transcription regulators that participate in plant growth, development and various procedures, including biotic and abiotic stress reactions. In this study, we identified 41 PtrDof members from Populus trichocarpa genomes and classified them into four groups. The conserved motifs and gene structures of some PtrDof genes belonging to the same subgroup were almost the same. The 41 PtrDof genes were dispersed on 18 of the 19 Populus chromosomes. Many key stress- or phytohormone-related cis-elements were discovered in the PtrDof gene promoter regions. Consequently, we undertook expression profiling of the PtrDof genes in leaves and roots in response to osmotic stress and abscisic acid. A total of seven genes (PtrDof14, 16, 25, 27, 28, 37 and 39) in the Populus Dof gene family were consistently upregulated at point in all time in the leaves and roots under osmotic and abscisic acid (ABA) stress. We observed that 12 PtrDof genes could be targeted by 15 miRNAs. Moreover, we mapped the cleavage site in PtrDof30 using the 5’RLM-RACE. The results showed that PtrDofs may have a role in resistance to abiotic stress in Populus trichocarpa.

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“…Other seedlings were exposed to a period of either 4 or 40 °C in a chamber delivering a 16 h photoperiod and 50 μmol·m −2 ·s −1 light, after which the second true leaves were sampled [37]. The wounding treatment involved cutting the second true leaf.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

Song

Jiang

et al. 2014

IJMS

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WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior.

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A Chrysanthemum Heat Shock Protein Confers Tolerance to Abiotic Stress

Cited by 89 publications

References 48 publications

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants

Characterization of Dof Transcription Factors and Their Responses to Osmotic Stress in Poplar (Populus trichocarpa)

Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

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