A Chronic Delayed Hypersensitivity Reaction in the Guinea Pig Colon

Bicks, Richard O.; Rosenberg, Elizabeth

doi:10.1016/s0016-5085(64)80003-2

Cited by 48 publications

(9 citation statements)

References 12 publications

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“…The pathological findings and time sequence were not typical of those seen in cutaneous delayed sensitivity reactions, but these differences may be due to the tissue involved. A chronic delayed hypersensitivity reaction has been described in the guinea-pig colon (Bicks and Rosenberg, 1964), but has not yet been described in the colon of man. It has been suggested that ulcerative colitis lymphocytes carry an antibody which reacts with colonic mucosa (Broberger, 1964), and fluorescent antibody stains have shown fluorescence in the cellular infiltrate of rectal biopsies from cases of ulcerative colitis (Kirsner, 1965).…”

Section: Resultsmentioning

confidence: 99%

Rectal reaction to injected ulcerative colitis leucocytes and plasma.

Fink¹,

Donnelly²,

Jablokow³

1967

Gut

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EDITORIAL COMMENT In patients with ulcerative colitis viable leucocytes from their peripheral blood injected into the rectal mucosa caused a local reaction which was not seen after injection with plasma, a phenomenon which needs further study.Leucocytes from the peripheral blood of patients with chronic ulcerative colitis are cytotoxic for foetal colon cells in tissue culture (Perlmann and Broberger, 1963). This report describes the effect of autologous ulcerative colitis leucocytes and cell-free plasma upon intact rectal mucosa. MATERIALS AND METHODSPATIENTS Seventeen ambulatory adult males with wellestablished diagnoses of chronic ulcerative colitis were studied. Rectal biopsies were compatible in each case. Patients were chosen who were in clinical remission, and without friable or ulcerating mucosa at the time of the investigation. Fifteen patients had been taking salicylazosulphapyridine (Azulfidine) for six to 24 months. None had been taking adrenal steroids during the preceding three months (Table I). Four patients with Laennec's cirrhosis and three with duodenal ulcer were also tested.PREPARATION OF MATERIAL Autologous leucocytes were obtained from venous blood by the method of Friedman, Bardawil, Merril, and Hanau (1960). A 30 ml. syringe containing 100 mg. of heparin (Liquaemin sodium, Organon) and 400 mg. of dextran in 20% aqueous solution was used to collect 27 ml. of blood. The needle was capped and the syringe placed in a vertical position at 4°C. for one hour. The buffy coat and plasma layers were then expressed into a centrifuge tube and centrifuged at 1,000 r.p.m. for 10 minutes. The supernatant clear plasma was pipetted off and approximately 1 ml. of cellular sediment-containing plasma was left. This 1 ml. contained 2.5 x 107 white blood cells, platelets, and a small number of red blood cells. In seven experiments, 0-5 ml. of this fluid was transferred to a sonic oscillator1 and oscillated at 9 kc. per second for 10 minutes. These 'Raytheon magnetostriction sonic oscillator model S-102A. preparations were examined by direct phase microscopy and repeatedly oscillated until all the cells seen were pyknotic and showed marked morphological change. These suspensions were used for injection when the morphological changes fulfilled the criteria of Schrek (1958) for non-viability, and are henceforth referred to as 'non-viable leucocytes' in this report. An aliquot of the supernatant plasma was placed in a Neubauer counting chamber and scanned for white blood cells. This plasma was re-centrifuged if necessary and used for injection when the microscopic examination failed to demonstrate leucocytes. This plasma is hereafter referred to as leucocyte-free.PROCEDURE Two hours after a 135 ml. sodium biphosphate enema (Fleet)2, 33 separate injections of 0-1 ml. of leucocyte-free plasma and of 0.1 ml. of plasma containing 2-5 x 106f intact leucocytes were made into two areas of rectal mucosa approximately 9 cm. from the anus. The lowest valve of Houston was taken as a reference point, and the injections were gi...

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Section: Resultsmentioning

confidence: 99%

Rectal reaction to injected ulcerative colitis leucocytes and plasma.

Fink¹,

Donnelly²,

Jablokow³

1967

Gut

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“…Rat UC Model was established by 2,4-dinitrochlorobenzene (DNCB) immunization and acetic acid local enema [3][4][5][6][7][8][9] . After hair on the rats' nape was removed with Na 2 S (100 g/L), 0.25 mL acetone solution with DNCB content of 20 g/L (5 drops) was dripped on the rats' back once a day for 14 days.…”

Section: Establishment Of Rat Uc Modelmentioning

confidence: 99%

Effect of Wumeiwan on cytokines TNF-α, IL-6, IL-8, IL-10 and expression of NF-κBp65 in rats with ulcerative colitis

Fan

Shen

Tang

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The effects of Wumeiwan (WMW) on TNF-alpha, IL-6, IL-8, IL-10 and NF-kappaBp65 in rats with ulcerative colitis (UC) were investigated, the curative effectiveness of WMW vs salicylazosulfapyridine (SASP) was compared, and the action mechanism was analyzed. Fifty-Six Sprague-Dawley (SD) rats were randomly divided into four groups (n=14 in each group, with equal ratio of male and female): normal control group, model group, SASP group, and WMW group. Except normal control group, the rat UC models in the remaining three groups were established using the method of 2.4-dinitrochlorobenzene (DNCB) immunization and acetic acid local enema. The rats in model group, SASP group, and WMW group were treated with distilled water, SASP, and WMW respectively. The changes in the symptoms and signs were observed, and levels of IL-6, IL-8, TNF-alpha, IL-10 and the expression of NF-kappaBp65 in the colonic tissues were statistically analyzed. The results showed that the levels of IL-6, IL-8, and TNF-alpha were significantly increased (P<0.01), while those of IL-10 significantly reduced (P<0.01) after establishment of rat UC models as compared with normal control group. The levels of IL-6, IL-8, and TNF-alpha were obviously lower, but the level of IL-10 was obviously higher in WMW and SASP groups than those in model group (P<0.05). The levels of IL-6, IL-8, and TNF-alpha were lower, while the level of IL-10 was higher in WMW group than in SASP group. NF-kappaBp65 was expressed negatively or weakly in normal colonic tissues. The positive expression rate of NF-kappaBp65 in WMW group and SASP group was obviously lower than in model group (P<0.01), and there was significant difference between WMW group and SASP group (P<0.05). It was concluded that rat UC model was established successfully. WMW could up-regulate the expression of IL-10, down-regulate the expression of TNF-alpha, IL-6, IL-8, and inhibit the NF-kappaBp65 activity to adjust immune function, indicating WMW had better curative effects on UC in rats.

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“…Despite the fact that some workers have succeeded in producing a type of delayed hypersensitivity reaction in the colon of the experimental animal (Rosenberg & Fischer, 1964;Bicks & Rosenberg, 1964), the position of allergens in the aetiology of ulcerative colitis is also somewhat uncertain at the present time. But it would be a serious omission to dismiss this subject without dealing in some detail with the potential allergen which has been most widely discussed during the last 40 years, namely cow's milk.…”

Section: Allergymentioning

confidence: 97%

Ulcerative colitis: definition, historical background, aetiology, diagnosis, naturel history and local complications

Dombal

1968

Postgraduate Medical Journal

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A Chronic Delayed Hypersensitivity Reaction in the Guinea Pig Colon

Cited by 48 publications

References 12 publications

Rectal reaction to injected ulcerative colitis leucocytes and plasma.

Rectal reaction to injected ulcerative colitis leucocytes and plasma.

Effect of Wumeiwan on cytokines TNF-α, IL-6, IL-8, IL-10 and expression of NF-κBp65 in rats with ulcerative colitis

Ulcerative colitis: definition, historical background, aetiology, diagnosis, naturel history and local complications

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