A Chromosome Phylogeny of the Droseraceae by Using CMA-DAPI Fluorescent Banding.

Hoshi, Yoshikazu; Kondo, Katsuhiko

doi:10.1508/cytologia.63.329

Cited by 19 publications

(9 citation statements)

References 19 publications

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“…The chromosomes did not obviously visualize any localizedcentromere. The results of chromosome number and feature were same to those of previous reports (Hoshi and Kondo 1998). Using base specific fluorescent staining, CMA positive bands were observed at metaphase (Fig 3c).…”

Section: Resultssupporting

confidence: 89%

Nucleotide sequence variation was unexpectedly low in an endangered species, Aldrovanda vesiculosa L. (Droseraceae)

2006

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ABSTRACT. Using fluorescence in situ hybridization (FISH) and polymerase chain reaction direct sequence methods, 45S ribosomal DNA (rDNA) loci on somatic chromosomes were detected and partial sequence of 45S rDNA was determined in Aldrovanda vesiculosa, respectively. Analysis of FISH showed tree to five 45S rDNA signals in interphase nuclei, and four signals at metephase stage. All FISH signals at metaphase stage were located at the terminal region of four chromosomes. Among six populations, completely homologue sequence result was supplied from Clustal W analysis. After determining sequences of 5.8S and internal transcribed spacer (ITS) in 45S rDNA of six populations, a comparative study with these sequences was carried out to infer genetic diversity in A. vesiculosa.

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Section: Resultssupporting

confidence: 89%

Nucleotide sequence variation was unexpectedly low in an endangered species, Aldrovanda vesiculosa L. (Droseraceae)

2006

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“…Although there is great variability in heterochromatin distribution patterns across angiosperms, discontinuous and extreme changes within a related group of species are uncommon (HOSHI and KONDO 1998;GUERRA 2000). In most genera for which data are available for at least fi ve species, the number of bands and the amount of heterochromatin varied, but the general patterns were relatively conserved (e.g., Vigna, Fabaceae: GALASSO et al 1996;Citrus, Rutaceae: MIRANDA et al 1997;Clivia, Amaryllidaceae: RAN et al 1999;Cestrum, Solanaceae: FREGONEZI et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Karyotypes and ﬂuorescent chromosome banding patterns in southern AfricanLycium(Solanaceae)

et al. 2010

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-Chromosomal analyses of several southern African Lycium species resulted in diploid (2n=24: L. amoenum, L. bosciifolium, L. ferocissimum, L. oxycarpum, L. tenue), tetraploid (2n=48: L. gariepense, L. strandveldense, L. hantamense), and hexaploid (2n=72: L. tetrandrum) counts. Chromosomes in all species were short (mean length = 2.00 μm; mean haploid genome length = 23.77 μm). Further, all species shared a highly symmetrical karyotype formula with 10 m pairs and 2 sm pairs, except L. bosciifolium with only one sm pair. The fi rst m pair had a terminal microsatellite on the short arms. Fluorescent chromosome banding patterns with CMA/ DAPI staining in the diploid species showed NOR-associated heterochromatin in the fi rst satellited pair. The tetraploids L. gariepense and L. hantamense had two chromosome pairs with a CMA + /DAPI -terminal band. Phylogenetic studies have shown that the southern African species are included in a monophyletic group including all Old World Lycium; these species are likely of recent origin and, despite ploidy differences, karyotypes are remarkably similar among species. Available data for South American, Asian, and southern African Lycium indicate that there is a common pattern of mostly m chromosomes in which a few cryptic chromosomal rearrangements may have occurred, suggesting that karyotypic orthoselection has preserved similar patterns among species. Thus, these data imply that speciation in Lycium was not accompanied by changes in chromosomal morphology.

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“…Even though there is a great variability in heterochromatin distribution patterns, discontinuous and extreme changes within a related group of species are not common (Hoshi andKondo 1998, Guerra 2000). In most genera in which data are available for at least five species, the numbers of bands and the heterochromatin amount varied, but the general patterns were relatively conserved [e.g.…”

Section: Discussionmentioning

confidence: 99%

Karyotypes and fluorescent chromosome banding in Pyrrhocactus (Cactaceae)

2008

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Summary. Karyotypes of the seven Pyrrhocactus species were studied for the first time with Feulgen staining and CMA/DAPI banding. All showed 2n = 22 (x = 11). The karyotypes were symmetrical with 9m + 2sm pairs, excepting P. catamarcensis with 8m + 2sm + 1st pairs. They had a terminal microsatellite on short arms of pair #1. Pyrrhocactus bulbocalyx possessed a second satellited pair (#2) exclusively detected with Feulgen. Increasing asymmetry was associated with a decline in karyotype size. Fluorochrome banding, applied for the first time in Cactaceae, revealed that nucleolar chromosome pair #1 had one CMA + /DAPI -terminal band in all species related to the nucleolar organizing region; additional pericentromeric bands were found. A pattern of homogeneous sized chromosomes with median and submedian centromeres is conserved in the genus. However, karyotypes can be distinguished by a combination of cytogenetic features. Species diversification in Pyrrhocactus has not been associated with large chromosome rearrangements or polyploidy, but with cumulative small and cryptic structural changes.

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A Chromosome Phylogeny of the Droseraceae by Using CMA-DAPI Fluorescent Banding.

Cited by 19 publications

References 19 publications

Nucleotide sequence variation was unexpectedly low in an endangered species, Aldrovanda vesiculosa L. (Droseraceae)

Nucleotide sequence variation was unexpectedly low in an endangered species, Aldrovanda vesiculosa L. (Droseraceae)

Karyotypes and ﬂuorescent chromosome banding patterns in southern AfricanLycium(Solanaceae)

Karyotypes and fluorescent chromosome banding in Pyrrhocactus (Cactaceae)

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