A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate

Li, Xiang; Leong, Susanna Su Jan

doi:10.1016/j.chroma.2011.04.017

Cited by 13 publications

(10 citation statements)

References 40 publications

(40 reference statements)

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“…Functional properties of bioactive peptides are determined by its unique three-dimensional (3D) structure, and further, secondary structure rather than tertiary structure is the dominant factor affecting the binding characteristics of the peptides (Alaiz et al 1992;Chan and Chen 2004;. Consequently, the purification criteria proposed so far have included MW or content of hydrophobic or cationic residues as the key factors (Li and Leong 2011;). …”

Section: Protein-derived Antioxidant Peptides (Bioactive Peptides)mentioning

confidence: 99%

Food Nanoscience and Nanotechnology

Hernández‐Sánchez¹,

Fidel²,

Gutiérrez-Ĺopez³

2015

Food Engineering Series

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Springer's Food Engineering Series is essential to the Food Engineering profession, providing exceptional texts in areas that are necessary for the understanding and development of this constantly evolving discipline. The titles are primarily reference-oriented, targeted to a wide audience including food, mechanical, chemical, and electrical engineers, as well as food scientists and technologists working in the food industry, academia, regulatory industry, or in the design of food manufacturing plants or specialized equipment. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper PrefaceFood nanoscience and nanotechnology are emergent disciplines that have grown enormously in the last years due to the attractive properties that a number of foodrelated materials have at the nanoscale. Understanding basic principles of such properties constitutes the basis for building a robust knowledge base for developing products with improved and novel characteristics.Understanding fundamentals of food nanotechnology represents a huge challenge for universities, industries and the public sector. The complex mechanisms involved in the research, development, production and legislation of food nanoproducts are studied under multi-and inter-disciplinary scopes. Bearing this in mind, this book was conceived.This book aims to contribute to basic and applied knowledge on these fields by presenting recent advances in selected topics of food nanoscience and nanotechnology, and is divided into a total of 17 chapters. The first chapter presents an overview to the field; the following chapter discusses general techniques for studying foodrelated nanomaterials, followed by six chapters on specific techniques for preparing food nanomaterials while the next contribution on dispersed systems illustrates this important and vast field. The book continues with three chapters on food nanocomposites, including those for packing purposes, and two chapters on advanced aspects of food nan...

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Section: Protein-derived Antioxidant Peptides (Bioactive Peptides)mentioning

confidence: 99%

Food Nanoscience and Nanotechnology

Hernández‐Sánchez¹,

Fidel²,

Gutiérrez-Ĺopez³

2015

Food Engineering Series

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“…The main peptide families of interest, defensins, and cathelicidins, are synthesized as precursor‐proteins that are proteolytically cleaved to produce mature peptides . These precursors protect host cells from the cytotoxic effects of the mature peptides, and fusion tags are used to mimic these precursors, preventing cytotoxicity, and successfully generating functional peptides.…”

Section: Tags For Functional Activitymentioning

confidence: 99%

“…This may be due to the large size of GST (28 kDa), especially in relation to the small antimicrobial peptides. MBP has been used for the production of Human β‐defensin 25 (hBD25) and Human β‐defensin 28 (hBD28), but both required refolding steps to recover fusion protein from aggregates in purification …”

Section: Tags For Functional Activitymentioning

confidence: 99%

To fuse or not to fuse: What is your purpose?

et al. 2013

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Since the dawn of time, or at least the dawn of recombinant DNA technology (which for many of today's scientists is the same thing), investigators have been cloning and expressing heterologous proteins in a variety of different cells for a variety of different reasons. These range from cell biological studies looking at protein-protein interactions, post-translational modifications, and regulation, to laboratory-scale production in support of biochemical, biophysical, and structural studies, to large scale production of potential biotherapeutics. In parallel, fusion-tag technology has grown-up to facilitate microscale purification (pull-downs), protein visualization (epitope tags), enhanced expression and solubility (protein partners, e.g., GST, MBP, TRX, and SUMO), and generic purification (e.g., His-tags, streptag, and FLAG TM -tag). Frequently, these latter two goals are combined in a single fusion partner. In this review, we examine the most commonly used fusion methodologies from the perspective of the ultimate use of the tagged protein.That is, what are the most commonly used fusion partners for pull-downs, for structural studies, for production of active proteins, or for large-scale purification? What are the advantages and limitations of each? This review is not meant to be exhaustive and the approach undoubtedly reflects the experiences and interests of the authors. For the sake of brevity, we have largely ignored epitope tags although they receive wide use in cell biology for immunopreciptation.

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“…Most of the carrier proteins are then cleaved and separated from AMPs. The available carrier proteins and cleavable linkers have been reviewed by Li et al (Li 2011). Deng et al reviewed different types of cell factories used to produce AMPs, including Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisae (Deng et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Recent achievements and perspectives for large-scale recombinant production of antimicrobial peptides

Wibowo

Zhao

2018

Appl Microbiol Biotechnol

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Antibiotic resistance poses a growing threat to global public health. It is urgent to develop new alternative antibiotics. Antimicrobial peptide (AMP) is a diverse class of natural occurring molecules that constitute immune systems of living organisms. More than 2,500 AMPs have been identified and isolated from natural sources. Compared to conventional antibiotics, AMPs exhibit antimicrobial activities against a broad spectrum of microorganisms including bacteria, fungi, and even viruses. More importantly, the unique antimicrobial mechanisms of AMPs make it difficult for microorganisms to develop resistance. Therefore, it is very promising to develop AMPs as high-value antimicrobial candidates. This Mini-Review provides an update of recent progresses in recombinant production of AMPs after fusion of AMP with carrier proteins and their scale-up. Key factors including selection of expression host and fusion tags are firstly introduced, followed by subsequent discussions on purification of fusion proteins and recovery of antimicrobial peptides. The scale production of AMPs is also explored.

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A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate

Cited by 13 publications

References 40 publications

Food Nanoscience and Nanotechnology

Food Nanoscience and Nanotechnology

To fuse or not to fuse: What is your purpose?

Recent achievements and perspectives for large-scale recombinant production of antimicrobial peptides

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