A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments

Liu, Jun; Last, Robert L.

doi:10.1073/pnas.1712206114

Cited by 45 publications

(34 citation statements)

References 59 publications

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“…The thylakoid lumenal 19 kDa protein, from membrane part (GO:0044425) in the cellular component, plays an essential role in plant photosynthesis. In Arabidopsis, loss of function of this protein causes the interruption of the photosystem II (PSII) repair process under changing light conditions, leading to decreased photosynthesis efficiency [18]. In our study, this protein was significantly down-regulated, indicating that decreased photosynthetic efficiency might be one of the responses of alligator weed in adaption to low potassium stress.…”

Section: Go Term and Kegg Pathway Analyses Of Daps Under Lk Stressmentioning

confidence: 52%

Quantitative Proteomic Analysis of Alligator Weed Leaves Reveals That Cationic Peroxidase 1 Plays Vital Roles in the Potassium Deficiency Stress Response

Lyu

et al. 2020

IJMS

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Alligator weed is reported to have a strong ability to adapt to potassium deficiency (LK) stress. Leaves are the primary organs responsible for photosynthesis of plants. However, quantitative proteomic changes in alligator weed leaves in response to LK stress are largely unknown. In this study, we investigated the physiological and proteomic changes in leaves of alligator weed under LK stress. We found that chloroplast and mesophyll cell contents in palisade tissue increased, and that the total chlorophyll content, superoxide dismutase (SOD) activity and net photosynthetic rate (PN) increased after 15 day of LK treatment, but the soluble protein content decreased. Quantitative proteomic analysis suggested that a total of 119 proteins were differentially abundant proteins (DAPs). KEGG analysis suggested that most represented DAPs were associated with secondary metabolism, the stress response, photosynthesis, protein synthesis, and degradation pathway. The proteomic results were verified using parallel reaction monitoring mass spectrometry (PRM–MS) analysis and quantitative real-time PCR (qRT-PCR)assays. Additional research suggested that overexpression of cationic peroxidase 1 of alligator weed (ApCPX1) in tobacco increased LK tolerance. The seed germination rate, peroxidase (POD) activity, and K+ content increased, and the hydrogen peroxide (H2O2) content decreased in the three transgenic tobacco lines after LK stress. The number of root hairs of the transgenic line was significantly higher than that of WT, and net K efflux rates were severely decreased in the transgenic line under LK stress. These results confirmed that ApCPX1 played positive roles in low-K+ signal sensing. These results provide valuable information on the adaptive mechanisms in leaves of alligator weed under LK stress and will help identify vital functional genes to apply to the molecular breeding of LK-tolerant plants in the future.

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Section: Go Term and Kegg Pathway Analyses Of Daps Under Lk Stressmentioning

confidence: 52%

Quantitative Proteomic Analysis of Alligator Weed Leaves Reveals That Cationic Peroxidase 1 Plays Vital Roles in the Potassium Deficiency Stress Response

Lyu

et al. 2020

IJMS

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“…Isoprene also up-regulated MPH1 and MPH2 gene expression in both Arabidopsis and tobacco. MPH1 is a thylakoid membrane protein that is required for the accumulation of PSII core proteins (Liu and Last, 2015b); MPH2 is a stromal protein essential for PSII repair and regeneration of dimeric functional PSII supercomplexes (Liu and Last, 2017). Both proteins have been shown to be essential to stabilize PSII, especially under high and fluctuating light stress (Liu and Last, 2015a, 2015b.…”

Section: Isoprene Executes Its Protective Functions By Altering Exprementioning

confidence: 99%

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

et al. 2019

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Isoprene synthase converts dimethylallyl diphosphate to isoprene and appears to be necessary and sufficient to allow plants to emit isoprene at significant rates. Isoprene can protect plants from abiotic stress but is not produced naturally by all plants; for example, Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) do not produce isoprene. It is typically present at very low concentrations, suggesting a role as a signaling molecule; however, its exact physiological role and mechanism of action are not fully understood. We transformed Arabidopsis with a Eucalyptus globulus isoprene synthase. The regulatory mechanisms of photosynthesis and isoprene emission were similar to those of native emitters, indicating that regulation of isoprene emission is not specific to isoprene-emitting species. Leaf chlorophyll and carotenoid contents were enhanced by isoprene, which also had a marked positive effect on hypocotyl, cotyledon, leaf, and inflorescence growth in Arabidopsis. By contrast, leaf and stem growth was reduced in tobacco engineered to emit isoprene. Expression of genes belonging to signaling networks or associated with specific growth regulators (e.g. gibberellic acid that promotes growth and jasmonic acid that promotes defense) and genes that lead to stress tolerance was altered by isoprene emission. Isoprene likely executes its effects on growth and stress tolerance through direct regulation of gene expression. Enhancement of jasmonic acid-mediated defense signaling by isoprene may trigger a growth-defense tradeoff leading to variations in the growth response. Our data support a role for isoprene as a signaling molecule.

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“…Arabidopsis mutants on TEF14 and CYN38 orthologs fail to assembly and repair PSII [29,31]. Chlamydomonas CDJ1 organizes chloroplast HSPs under heat stress [32] and its accumulation under UV-C pointed to an enhanced protection of photosynthetic proteins and complexes.…”

Section: Low Intensity Uv-c Irradiation Modulated Thylakoid Electron mentioning

confidence: 99%

Low UV-C stress modulates Chlamydomonas reinhardtii biomass composition and oxidative stress response through proteomic and metabolomic changes involving novel signalers and effectors

Colina

Carbó

Meijón

et al. 2020

Preprint

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Background: The exposition of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Despite UV-C shares these effects with UV-A/B but at much lower dosages, UV-C sensing and signal mechanisms are still mostly unknown. Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtii –a simple Plantae model– into the first 24 h after a short and low intensity UV-C irradiation in order to reconstruct the microalgae response system to this stress. Results : The microalgae response was characterized by increased redox homeostasis, ROS scavenging and protein damage repair/avoidance elements. These processes were upregulated along others related to the modulation of photosynthetic electron flux, carbon fixation and C/N metabolism. These changes, attributed to either direct UV-C-, ROS- or redox unbalances-associated damage, trigger a response process involving novel signaling intermediaries and effectors as the translation modulator FAP204, a PP2A like protein and a novel DYRK kinase. These elements were found linked to the modulation of Chlamydomonas biomass composition (starch accumulation) and proliferation, within an UV-C response probably modulated by different epigenetic factors.Conclusion: Chosen multiomics integration approach was able to describe many fast changes, including biomass composition and ROS stress tolerance, as a response to a low intensity UV-C stress. Moreover, the employed omics and systems biology approach placed many previously unidentified protein and metabolites at the center of these changes. These elements would be promising targets for the characterization of this stress response in microalgae and plants and the engineering of more productive microalgae strains.

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A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments

Cited by 45 publications

References 59 publications

Quantitative Proteomic Analysis of Alligator Weed Leaves Reveals That Cationic Peroxidase 1 Plays Vital Roles in the Potassium Deficiency Stress Response

Quantitative Proteomic Analysis of Alligator Weed Leaves Reveals That Cationic Peroxidase 1 Plays Vital Roles in the Potassium Deficiency Stress Response

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

Low UV-C stress modulates Chlamydomonas reinhardtii biomass composition and oxidative stress response through proteomic and metabolomic changes involving novel signalers and effectors

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