A chloroplast-targeted DnaJ protein contributes to maintenance of photosystem II under chilling stress

Kong, Fanying; Deng, Yong-Sheng; Zhou, Bin; Wang, Guodong; Yu, Wang; Meng, Qingwei

doi:10.1093/jxb/ert357

Cited by 141 publications

(114 citation statements)

References 59 publications

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“…In addition, a Dnaj-like (Spot 30) was also up-regulated under all Sb stress conditions. Dnaj proteins act as co-chaperones in protein homeostasis and protein complex stabilization under adverse conditions (Kong et al, 2014). Zhang et al (2000) showed that Dnaj-like proteincoding genes in Phaseolus vulgaris was up-regulated under Hg and Cd treatment, indicating that DnaJ-like protein may play a significant effect in maintaining cell structure and function under heavy metal stress.…”

Section: Stress Responsementioning

confidence: 99%

Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

Xue

Ren

et al. 2015

Environmental Pollution

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Section: Stress Responsementioning

confidence: 99%

Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

Xue

Ren

et al. 2015

Environmental Pollution

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“…At least 63 DnaJ genes were found in the tomato genome in the National Center for Biotechnology Information (NCBI). We isolated a tomato ( Lycopersicon esculentum ) chloroplast‐targeted type III DnaJ protein gene, LeCDJ1 , then constructed LeCDJ1 overexpressing plants, and proved its role in maintaining PSII under chilling stress in the previous study (Kong et al ). In the present study, we further proved that overexpression of LeCDJ1 increased heat tolerance by alleviating the photoinhibition of PSII and improving the activities of antioxidant enzymes in tomato chloroplast.…”

Section: Introductionmentioning

confidence: 99%

LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes

Kong

Deng

Wang

et al. 2014

JIPB

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The roles of a tomato (Lycopersicon esculentum) chloroplast-targeted DnaJ protein (LeCDJ1) were investigated using wild-type (WT) and sense transgenic tomatoes. The LeCDJ1 expression was upregulated by 38 °C, 42 °C, 45 °C, NaCl, PEG, methyl viologen (MV) and hydrogen peroxide (H2O2), but not by 30 °C and 35 °C. Meanwhile, LeCDJ1 was involved in the response of plants to abscisic acid (ABA). Under heat stress, the sense plants showed better growth, higher chlorophyll content, lower malondialdehyde (MDA) accumulation and relative electrical conductivity (REC), and also less PSII photoinhibition than WT. Interestingly, the sense plants treated with streptomycin (SM), an inhibitor of organellar translation, still showed higher maximum photochemistry efficiency of PSII (Fv/Fm) and D1 protein levels than the SM-untreated WT, suggesting that the protective effect of LeCDJ1 on PSII was, at least partially, independent of D1 protein synthesis. Furthermore, the relatively lower superoxide radical (O2(•-)) and H2O2 levels in the sense plants were considered to be due to the higher ascorbate peroxidase (APX) and superoxide dismutase (SOD) activity, which seemed unlikely dependent on their transcription level. These results indicated that LeCDJ1 overexpression facilitated heat tolerance in transgenic tomatoes.

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“…Vegetables produced in off-seasons are mainly grown in solar-energy greenhouses during winter-spring period when outside temperature is low. Low temperature is one of the major abiotic stresses limiting plant growth, development and productivity (Liu et al 2009(Liu et al , 2011Hu et al 2010;Kong et al 2013). Pepper (Capsicum annuum L.) is an important thermophilic crop and the optimal growth diurnal temperature is 28 and 18°C (day and night) (Allen and Ort 2001).…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroid alleviates chilling-induced oxidative stress in pepper by enhancing antioxidation systems and maintenance of photosystem II

Yang

Gan

et al. 2015

Acta Physiol Plant

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To explore regulated mechanisms of Brassinosteroids-induced chilling tolerance, we studied the involvement of foliar sprayed 24-epibrassinolide (EBR) in the growth, lipid peroxidation, distribution of absorbed energy and excitation energy, chlorophyll fluorescence characteristics and antioxidant defense system of pepper seedlings under chilling stress. We found that low temperature retarded the growth of pepper seedlings, but foliar spray of EBR solution markedly improved the photoinhibition by increasing maximum quantum efficiency of photosystem II (F v /F m ), the actual photochemical efficiency of photosystem II, photochemical quenching coefficient and the efficiency of excitation capture of open PSII center (F v 0 /F m 0 ). Likewise, EBR increased the fraction of photochemical efficiency (P) and reduced the fraction of antenna heat dissipation (D) and excess energy (E). Low temperature led the increase in end product of lipid peroxidation and the content of H 2 O 2 , O 2 -and OH -, and it caused the occurrence of oxidative stress. The activities of antioxidative enzymes including superoxide dismutase, peroxidase, catalase and ascorbate peroxidase, and contents of ascorbic acid and reduced glutathione were significantly improved by EBR during low temperature stress. The application of EBR also markedly increased the contents of proline, soluble sugar and protein under low temperature. EBR significantly reinforced antioxidant defense system, and it can be reflected through the reduced accumulation of harmful reactive oxygen species and MDA in pepper seedlings. Overall, these results suggest that EBR increases the tolerance of pepper seedlings against chilling stress largely by optimizing distribution of absorbed energy and excitation energy and enhancing antioxidant defense system.Keywords 24-epibrassinolide Á Chilling stress Á Distribution of absorbed energy and excitation energy Á Antioxidant system Á Pepper Abbreviations BRs Brassinosteroids Chl Chlorophyll D Fraction of antenna heat dissipation DAB 3,3-diaminobenzidine E Excess energy EBR 24-epibrassinolide ETR Electron transport rate F v /F m Maximum quantum efficiency of PSII F v 0 /F m 0 Efficiency of excitation capture of open PSII center FW Fresh weight GSH Reduced glutathione MDA Malondialdehyde OHHydroxyl radical O 2 -

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A chloroplast-targeted DnaJ protein contributes to maintenance of photosystem II under chilling stress

Abstract: A novel tomato chloroplast-targeted DnaJ protein, LeCDJ1 was found to contribute to the maintenance of photosystem II under chilling stress and this maintenance effect was, at least partially, independent of D1 protein synthesis

Cited by 141 publications

References 59 publications

Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes

Brassinosteroid alleviates chilling-induced oxidative stress in pepper by enhancing antioxidation systems and maintenance of photosystem II

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