A chloroplast-localized DEAD-box RNA helicaseAtRH3 is essential for intron splicing and plays an important role in the growth and stress response in Arabidopsis thaliana

The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1

“…Yeast two-hybrid (Y2H) and pull-down assays indicated that RH22 interacts with PRPL24 in the 50S ribosomal subunit and with a short fragment of the 23S rRNA. RH3 has been characterized in both Arabidopsis and maize (Zea mays; Asakura et al, 2012;Gu et al, 2014). The atrh3 null mutant is embryo-lethal, whereas a weak allele (atrh3-4) resulted in pale-green seedlings due to defects in splicing of group-II introns and reduced levels of the 50S ribosomal subunit owing to impaired production of 23S and 4.5S rRNAs Gu et al, 2014).…”

“…RH3 has been characterized in both Arabidopsis and maize (Zea mays; Asakura et al, 2012;Gu et al, 2014). The atrh3 null mutant is embryo-lethal, whereas a weak allele (atrh3-4) resulted in pale-green seedlings due to defects in splicing of group-II introns and reduced levels of the 50S ribosomal subunit owing to impaired production of 23S and 4.5S rRNAs Gu et al, 2014). A tobacco (Nicotiana benthamiana) RH58/VDL mutant is defective in plastid differentiation and plant morphogenesis (Wang et al, 2000).…”

DEAD-box RNA helicases and epigenetic control of abiotic stress-responsive gene expression

“…In plants, DEADbox RNA helicases have been associated with diverse molecular functions such as siRNA-mediated gene silencing, premRNA splicing, chloroplast/mitochondrial intron splicing, nonsense-mediated mRNA decay, mRNA export, ribosome biogenesis and exon-junction complex functions. [11][12][13][14][15][16][17][18][19][20][21][22] Furthermore, it has been shown that these helicases play roles in Keywords: abiotic stress, Arabidopsis thaliana, DEAD-box RNA helicases, epigenetic gene silencing, nucleolus, RNAdirected DNA methylation Abbreviations: ABA, abscisic acid; STRS, STRESS RESPONSE SUPPRESSOR; RdDM, RNA-directed DNA methylation. various biological processes including abiotic stress responses.…”

Analysis of the role of RNS2 and autophagy in the turnover of ribosomal RNA in Arabidopsis thaliana

“…AtHELPS seems to functions as a negative regulator during low potassium stress ). Other DEAD-box helicases have been implicated in a variety of physiological processes in plants including determination of cell fate, biotic and abiotic stress, gene silencing, and growth (Kobayashi et al 2007;Guan et al 2013;Gu et al 2014;Khan et al 2014;Tuteja et al 2014;Zhang et al 2014).…”