A child is not an adult: development of a new in vitro model of the toddler colon

Fournier, E; Denis, Sylvain; Dominicis, Alessandra; Wiele, Tom Van de; Alric, Monique; Mercier‐Bonin, Muriel; Etienne‐Mesmin, Lucie; Blanquet-Diot, Stéphanie

doi:10.1007/s00253-022-12199-0

Cited by 5 publications

(5 citation statements)

References 95 publications

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“…During the critical period of the first 1000 days, the gut microbiota of infants remains immature in terms of composition and functionalities [35]. This microbiota immaturity has been successfully reproduced in the Tm-ARCOL model [46], with lower microbial diversity, higher abundance of infant-related microbial populations (e.g. Bifidobacteriaceae, Enterobacteriaceaea), higher acetate production and lower butyrate production in toddlers than in adults, consistent with in vivo data [69][70][71].…”

Section: Discussionsupporting

confidence: 72%

“…Due to major ethical constraints linked to clinical studies on young populations, but also the striking differences between human and rodent digestive physiology -including microbiota- [45], these experiments were carried out using an innovative in vitro approach, combining the Toddler mucosal Artificial Colon (Tm-ARCOL, This preprint research paper has not been peer reviewed. Electronic copy available at: https://ssrn.com/abstract=4229664 P r e p r i n t n o t p e e r r e v i e w e d [46]) simulating the colonic ecosystem specific to infants (6 months to 3 years) and a co-culture of intestinal epithelial and mucus-secreting goblet cells (Caco-2 and HT29-MTX cells, respectively). After a 2-week daily exposure to PE MPs, the structure and metabolic activities of the infant microbiome were determined by 16S metabarcoding and measurements of gas/SCFAs/VOCs/AhR activity, respectively.…”

Section: Mps Injected Intraperitoneally Into Pregnant Mice Resulted I...mentioning

confidence: 99%

“…The Tm-ARCOL is a one-stage fermentation system (MiniBio, Applikon, The Netherlands), operated under continuous conditions, which simulates both physicochemical (pH, retention time, ileal effluents supply and anaerobiosis) and microbial conditions (lumen and mucus associated microbiota) encountered in the colon of young infant [46]. This model is composed of a main bioreactor (luminal compartment) connected to an external glass compartment (mucosal compartment) containing mucin-alginate beads (Fig.…”

Section: Description and Set-up Of The Colonic Tm-arcol Modelmentioning

confidence: 99%

“…The mucin-alginate beads were stored at 4°C before introduction into the hermetically sealed glass compartment (total beads area of 556 cm²) connected to the bioreactor. Tm-ARCOL was set-up, based on in vivo data, to reproduce the average conditions found in the colon of a healthy toddler, as previously described [46]. Briefly, the composition of the ileal effluents was adjusted to reflect the specificities of the infant diet (Fig.…”

Section: Description and Set-up Of The Colonic Tm-arcol Modelmentioning

confidence: 99%

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Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

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Ratel

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et al. 2023

Journal of Hazardous Materials

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Section: Discussionsupporting

confidence: 72%

Section: Mps Injected Intraperitoneally Into Pregnant Mice Resulted I...mentioning

confidence: 99%

Section: Description and Set-up Of The Colonic Tm-arcol Modelmentioning

confidence: 99%

Section: Description and Set-up Of The Colonic Tm-arcol Modelmentioning

confidence: 99%

See 2 more Smart Citations

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Fournier

Ratel

Denis

et al. 2023

Journal of Hazardous Materials

Self Cite

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“…For example, media have been developed to mimic the diet of formula-fed infants [73][74][75] and African infants living in low-income countries. In contrast, when assessing the infant gut microbiota at weaning age, cultivation media are supplemented with dietary compounds from their first solid food [76,77] . Finally, it is necessary to carefully evaluate whether a particular test compound should undergo suitable pre-treatments mimicking upper gastrointestinal tract digestion (e.g., INFOGEST) prior to colon model incubation [69,78] .…”

Section: Selection Of Model Type and Operation According To The Resea...mentioning

confidence: 99%

In vitro human gut microbiota fermentation models: opportunities, challenges, and pitfalls

Isenring¹,

Bircher²,

Geirnaert³

et al. 2023

Microbiome Res Rep

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The human gut microbiota (HGM) plays a pivotal role in health and disease. Consequently, nutritional and medical research focusing on HGM modulation strategies as a means of improving host health is steadily increasing. In vitro HGM fermentation models offer a valid complement to human and animal studies when it comes to the mechanistic exploration of novel modulation approaches and their direct effects on HGM composition and activity, while excluding interfering host effects. However, in vitro cultivation of HGM can be challenging due to its high oxygen sensitivity and the difficulties of accurately modeling the physio-chemical complexity of the gut environment. Despite the increased use of in vitro HGM models, there is no consensus about appropriate model selection and operation, sometimes leading to major deficiencies in study design and result interpretation. In this review paper, we aim to analyze crucial aspects of the application, setup and operation, data validation and result interpretation of in vitro HGM models. When carefully designed and implemented, in vitro HGM modeling is a powerful strategy for isolating and investigating biotic and abiotic factors in the HGM, as well as evaluating their effects in a controlled environment akin to the gut. Furthermore, complementary approaches combining different in vitro and in vivo models can strengthen the design and interpretation of human studies.

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PETR: A novel peristaltic mixed tubular bioreactor simulating human colonic conditions

Vorländer,

Schultz,

Hoffmann

et al. 2023

Biotech & Bioengineering

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A novel bioreactor simulating human colonic conditions for in vitro cultivation of intestinal microbiota is presented. The PEristaltic mixed Tubular bioReactor (PETR) is modular designed and periodically kneaded to simulate intestinal peristalsis. The reactor is introduced, characterized from a bioprocess engineer's perspective and discussed in its ability to mimic colon conditions. PETR provides physiological temperature and appropriate anaerobic conditions, simulates intestinal peristalsis, and has a mean residence time of 32.8 ± 0.8 h comparable to the adult human colon. The single‐tube design enables a time‐constant and longitudinally progressive pH gradient from 5.5 to 7.0. Using a dialysis liquid containing high molecular weight polyethylene glycol, the integrated dialysis system efficiently absorbs short chain fatty acids (up to 60%) and water (on average 850 mL d−1). Cultivation of a typical gut bacterium (Bifidobacterium animalis) was performed to demonstrate the applicability for controlled microbiota cultivation. PETR is unique in combining simulation of the entire colon, peristaltic mixing, dialytic water and metabolite absorption, and a progressive pH gradient in a single‐tube design. PETR is a further step to precise replication of colonic conditions in vitro for reliable and reproducible microbiota research, such as studying the effect of food compounds, prebiotics or probiotics, or the development and treatment of infections with enteric pathogens, but also for further medical applications such as drug delivery studies or to study the effect of drugs on and their degradation by the microbiota.

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A child is not an adult: development of a new in vitro model of the toddler colon

Cited by 5 publications

References 95 publications

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

In vitro human gut microbiota fermentation models: opportunities, challenges, and pitfalls

PETR: A novel peristaltic mixed tubular bioreactor simulating human colonic conditions

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