A Chemometric-Assisted Colorimetric-Based Inexpensive Paper Biosensor for Glucose Detection

Abstract

This article reports a simple and inexpensive leak-proof paper pad with an initial selection of a paper substrate on the grounds of surface morphology and fluid absorption time. Herein, a drying method is used for glucose detection on a paper pad through colorimetric analysis, and the spot detection of glucose is analyzed by optimizing the HRP concentration and volume to obtain accurate results. The rapid colorimetric method for the detection of glucose on the paper pad was developed with a limit of detection (LOD) of 2.92 mmol L−1. Furthermore, the effects of the detection conditions were investigated and discussed comprehensively with the help of chemometric methods. Paper pads were developed for glucose detection with a range of 0.5–20 mM (apropos to the normal glucose level in the human body) and 0.1–0.5 M (to test the excessive intake of glucose). The developed concept has huge potential in the healthcare sector, and its extension could be envisioned to develop the reported paper pad as a point-of-care testing device for the initial screening of a variety of diseases.

Predictive Framework Development for User-Friendly On-Site Glucose Detection

“…The region between the outer edge of the inner circle and the inner edge of the outer circle was utilized as a boundary to establish a hydrophobic barrier. For a comprehensive description of the fabrication process, previous publication by the group can be referred …”

“…For a comprehensive description of the fabrication process, previous publication by the group can be referred. 27 Image Capturing. In order to achieve high classification performance, machine learning-based approaches require training with a suitable data set.…”

Catalytic Modification of Porous Two-Dimensional Ni-MOFs on Portable Electrochemical Paper-Based Sensors for Glucose and Hydrogen Peroxide Detection

“…Common detection methods for H 2 O 2 and Glu include spectrophotometry, chemiluminescence, titrimetric analysis, colorimetry, and chromatography; however, these methods generally suffer from low sensitivity, cumbersome operation steps, long process durations, and high costs, limiting their wide application [21][22][23]. The recently developed electrochemical method has the advantages of high specificity and sensitivity for real-time monitoring; it is also inexpensive and portable and involves a simple operation procedure [24,25].…”