A chemometric approach to identify the grape cultivar employed to produce nutraceutical fruit juice

Crupi, Pasquale; Bergamini, Carlo; Perniola, Rocco; Dipalmo, Tiziana; Clodoveo, Maria Lisa; Antonacci, Donato

doi:10.1007/s00217-015-2478-y

Cited by 15 publications

(10 citation statements)

References 31 publications

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“…In accordance with the literature, the cultivar was prevalently characterized by dihydroxylated anthocyanins, in particular, peonidin-3O-glucoside (Fig. 2), as typically found in pink and red colored table grape varieties [10,13,24]. Apart from the first sampling date, the treatment with PhCO 2 confirmed to be significantly related to ripening (p <0.001; F= 4.67).…”

Section: Resultssupporting

confidence: 84%

“…Anthocyanins were tentatively identified ( Fig. S2) by matching the elution pattern with those of the reference standards and data reported in the literature [13,22]; then, they were quantified according to the external standard method with a calibration curve obtained by the injection of 5 standard solutions at different concentrations (ranging from 50 to 300 μg/mL) of cyanidin-3-O-glucoside (R 2 = 0.9996). Results were expressed as μg cyanidin-3-Oglucoside equivalents per g of dry skins.…”

Section: Uv-vis and Hplc-dad Analysesmentioning

confidence: 99%

“…In this context, some varieties (i.e. 'Crimson Seedless') are known to fail in achieving the desired level of red color at harvesting, especially in very warm climates, maybe due to consistently high temperature in summer together with the narrow day/night temperature range [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Harpin Proteins Improve Bioactive Compounds Content in Crimson Seedless Table Grape

Crupi¹,

Debiase²,

Masi³

et al. 2019

TOBCJ

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Background: Frequently, in warm climates such as Mediterranean areas, a red-pink table grape ‘Crimson Seedless’ does not reach a good berry skin color; and an acceptable anthocyanin bioactive compounds content, responsible for the red color of berries. Harpin proteins are biotechnologically developed bio-activators that, if applied on plants during the growing period, trigger the expression of hundreds of genes among which those associated with the biosynthesis of bioactive compounds (such as anthocyanins). Aim: This research aimed at using harpin proteins to test their suitability in improving the grape skin color. Methods and Materials: Beta-harpin protein 1% p.a. (400 g/Ha) was applied to ‘Crimson Seedless’ vines three times at the beginning of veraison. Six samplings were carried out for both the treated and control grapes until commercial harvest. In the skin extracts, total and individual anthocyanins content was determined by UV-Vis and RP-HPLC-DAD analyses, respectively. Results: The collected results confirmed that the application of harpin proteins effectively stimulated the anthocyanin biosynthesis leading to make peonidin-3O-glucoside, cyanidin-3O-glucoside, and malvidin-3O-glucoside values from 2 to almost 10 folds higher in treated grapes than in control grapes (P < 0.05). Conclusion: Actually, harpin proteins improved the color of the berry skin, leading to a significantly higher concentration of anthocyanins in treated than in control grapes.

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Section: Resultssupporting

confidence: 84%

Section: Uv-vis and Hplc-dad Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Harpin Proteins Improve Bioactive Compounds Content in Crimson Seedless Table Grape

Crupi¹,

Debiase²,

Masi³

et al. 2019

TOBCJ

Self Cite

View full text Add to dashboard Cite

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“…However, their fragmentation patterns were almost identical. They provided the fragments at m/z 300.0 (deprotonated radical aglycone) and m/z 301.0 (aglycone) due to successive losses of glycoside fragments in MS 2 , and the ions at m/z 271 [M–glycosides–HCO] − , 255 [M–glycosides–C 2 H 5 O] − , 178.9, and 151.0 resulted from RDA fragmentation. The structures of these compounds were tentatively identified as rutin, isoquercitrin, and quercitrin .…”

Section: Resultsmentioning

confidence: 99%

HPLC–MS/MS determination of flavonoids in Gleditsiae Spina for its quality assessment

Jiang

Wang

et al. 2018

J of Separation Science

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Gleditsiae Spina, the thorn of Gleditsia sinensis Lam., has been used as an anti-inflammatory, anti-tumor, and anti-bacterial traditional medicine for hundreds of years in China. This study used high-performance liquid chromatography and tandem mass spectrometry combined with chemometric methods to allow the fast and accurate identification and quantification of the flavonoids compounds in Gleditsiae Spina, and created reliable criteria for accurate identification of Gleditsiae Spina and its adulterants. This research provides good evidence for the classification and quality evaluation of Gleditsiae Spina. Firstly, eight flavonoids compounds were detected and identified on the basis of their mass spectra, fragment characteristics, and comparison with published data. Then the mass spectroscopic fragmentation pathways of these compounds were determined and, in addition rutin, isoquercitrin, and quercitrin were detected in Gleditsiae Spina for the first time. The quantification was performed on a triple quadrupole tandem mass spectrometer in multi-reaction monitoring mode, and the baseline separation of the eight bioactive flavonoids components was achieved within 13 min. Furthermore, the proposed method was successfully applied for simultaneous quantitative determination of the eight Gleditsiae Spina compounds and adulterants obtained from different sources in China. Then, we built a classification model which showed a high level of accuracy predicting 100% of the samples, correctly.

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“…As observed in the work of Crupi et al (2015), where was used PCA and HCA tools to differentiate seven grape varieties used for juice production. Considering its contents of flavonoids, it was possible to separate them into two groups, thus tracing the origin of the cultivar used in grape juice.…”

Section: Exploratory Analysis Techniquesmentioning

confidence: 99%

Separation of Cultivars of Soybeans by Chemometric Methods Using Near Infrared Spectroscopy

Panero¹,

Silva²,

Panero³

et al. 2018

JAS

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Near Infrared (NIR) Spectroscopy technique combined with chemometrics methods were used to group and identify samples of different soy cultivars. Spectral data, collected in the range of 714 to 2500 nm (14000 to 4000 cm -1 ), were obtained from whole grains of four different soybean cultivars and were submitted to different types of pre-treatments. Chemometrics algorithms were applied to extract relevant information from the spectral data, to remove the anomalous samples and to group the samples. The best results were obtained considering the spectral range from 1900.6 to 2187.7 nm (5261.4 cm -1 to 4570.9 cm -1) and with spectral treatment using Multiplicative Signal Correction (MSC) + Baseline Correct (linear fit), what made it possible to the exploratory techniques Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) to separate the cultivars. Thus, the results demonstrate that NIR spectroscopy allied with de chemometrics techniques can provide a rapid, nondestructive and reliable method to distinguish different cultivars of soybeans.

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A chemometric approach to identify the grape cultivar employed to produce nutraceutical fruit juice

Cited by 15 publications

References 31 publications

Harpin Proteins Improve Bioactive Compounds Content in Crimson Seedless Table Grape

Harpin Proteins Improve Bioactive Compounds Content in Crimson Seedless Table Grape

HPLC–MS/MS determination of flavonoids in Gleditsiae Spina for its quality assessment

Separation of Cultivars of Soybeans by Chemometric Methods Using Near Infrared Spectroscopy

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