A chemiluminescent probe specific for singlet oxygen

Posner, Gary H.; Lever, John R.; Miura, Kyo; Lisek, Carol; Seliger, H. H.; Thompson, Ambler

doi:10.1016/0006-291x(84)90311-5

Cited by 32 publications

(13 citation statements)

References 19 publications

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“…Another specific probe is trans-1-(2'-methoxyvinyl)pyrene (MVP), which forms a blue (465 nm) chemiluminescence emitting dioxetane intermediate upon reacting with 1 O 2 [31]. Although known for more than 20 years and utilized in medical research [32], this probe has no documented plant application.…”

Section: Mvpmentioning

confidence: 99%

A comparative study of fluorescent singlet oxygen probes in plant leaves

Hideg

2008

Open Life Sciences

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Four fluorescent singlet oxygen sensors: DanePy, its oxalate salt, Singlet Oxygen Sensor Green and MVP, were infiltrated into tobacco leaves and tested for toxicity, subcellular localization, light sensitivity and capacity to trap the singlet oxygen produced in photoinhibition. For reference, a broad sensitivity free radical probe, TEMPO-9-AC, was also included. Photochemical yield was approximately 15% and 10% inhibited by Singlet Oxygen Sensor Green and MVP, respectively, but was not significantly affected by the other probes. Under photoinhibitory conditions, brought about by irradiating lincomycin-treated leaves with strong photosynthetically active radiation, DanePy and Singlet Oxygen Sensor Green were responsive. Singlet Oxygen Sensor Green was also reactive to low, non-photoinhibitory light exposure of the leaf, which was not characteristic to the other probes. MVP did not respond to singlet oxygen which can partly be explained by a possible attenuation of its blue emission in the leaf, as shown by the example TEMPO-9-AC. DanePy-oxalate did not respond to photosynthetic singlet oxygen due to lack of its penetration into photosynthetic tissue and hence could be useful in detecting any singlet oxygen which escapes from a chloroplast initiation site. DanePy was localized in the chloroplasts, while Singlet Oxygen Sensor Green was mainly found in the epidermal cells preferentially associated with the nucleus.

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Section: Mvpmentioning

confidence: 99%

A comparative study of fluorescent singlet oxygen probes in plant leaves

Hideg

2008

Open Life Sciences

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“…5B) (11). To further verify the results, we used 2 chemiluminescent probes, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA) and trans-1-(2Ј-methoxyvinyl)pyrene (MVP), which were developed to specifically detect superoxide and singlet oxygen, respectively (10,(12)(13)(14). The pretreatment of control neutrophils with DPI abolished the CLA chemiluminescence, suggesting the specificity of this probe for superoxide detection (Fig.…”

Section: Ozone Produced By Amino Acids Exhibits Bactericidal Activitymentioning

confidence: 99%

Ozone production by amino acids contributes to killing of bacteria

Yamashita¹,

Miyoshi²,

Arai

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Reactive oxygen species produced by phagocytosing neutrophils are essential for innate host defense against invading microbes. Previous observations revealed that antibody-catalyzed ozone formation by human neutrophils contributed to the killing of bacteria. In this study, we discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the wateroxidation pathway, at comparable level to antibodies. The resultant oxidant with the chemical signature of ozone exhibited significant bactericidal activity in our distinct cell-free system and in human neutrophils. The results also suggest that an oxidant with the chemical signature of ozone produced by neutrophils might potentiate a host defense system, when the host is challenged by high doses of infectious agents. Our findings provide biological insights into the killing of bacteria by neutrophils.host defense ͉ singlet oxygen ͉ neutrophil ͉ chronic granulomatous disease

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“…In chemiluminescence, the reaction of 1 O 2 with MVP emits light output at 465 nm (9), and that of O 2…”

Section: •−mentioning

confidence: 99%