A chemical synthesis of nicotinamide adenine dinucleotide (NAD+)

Abstract

A practical synthesis of nicotinamide mononucleotide (b-NMN) and a high yield coupling with AMP-morpholidate that also provides NAD + in an efficient manner are reported.

Enzymatic Synthesis and Structural Characterization of 13C, 15N-Poly(ADP-ribose)

“…A branch point (1‴) resonance expected at ~5.2 ppm 31 was not observed. Although, other groups reported ~2% branching for in vitro synthesis of PAR 18,25, and it is likely that the small number of branch points incorporated were below the threshold for detection. Analysis of the nuclease digest of the polymer did reveal evidence for a small percentage of branch points (Supplementary Figure 2).…”

Fluorescence Properties of Carba Nicotinamide Adenine Dinucleotide for Glucose Sensing

“…NAD, NADH were supplied from Roche (Germany). cNAD was synthesized following the protocol by Lee et al [28] and reduced to cNADH in analogy to the protocol by Panza et al [29] For each measurement NAD, cNAD, NADH and cNADH were dissolved in Tris buffer (0.1 m, pH 8.5) containing NaCl (0.2 m).…”

Novel nicotinamide adenine dinucleotide analogues as selective inhibitors of NAD+-dependent enzymes

“…20 The reaction was carried out in 4 days in a 0.2 M solution of MnCl 2 in formamide in presence MgSO 4 and was monitored by HPLC (SAX column, K 2 HPO 4 50 mM, 5% MeOH, pH 3.5). Purifications of the three dinucleotides 7ab and 7ba and 7bb were carried out on DEAE sepharose columns with a triethylammonium formate gradient.…”