A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Abstract

A specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods. Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP. Several physiochemical factors that may influence the assay performance were optimized in the assay. Under optimal conditions, a detection limit of 0.0094 ng mL −1 was obtained and good linearity was achieved within a range of 0.03-35.40 ng mL −1 . Satisfactory recovery of spiked samples (91.12-109.23%) was obtained and the coefficient of variation was acceptable. Finally, the detection results of BaP in environmental and food samples were consistent with those obtained using high-performance liquid chromatography. The proposed immunoassay is reliable and has great potential for detecting trace amounts of BaP.

Polycyclic Aromatic Hydrocarbons’ Impact on Crops and Occurrence, Sources, and Detection Methods in Food: A Review

“…Confirmation and relevance with HPLC-FLD has confirmed the ic-ELISA method as a feasible tool that lays a foundation for the development and application of PAH residue detection kit in aquatic products. In a separate study, Ma et al [210] applied a streptavidin-horseradish peroxidase-based ELISA method utilizing synthesized polyclonal antibody (pAb) for the sensitive monitoring of BaP in environmental and food samples (e.g., vegetables, cereals, and barbecue food). This method achieved a low LOD of 0.0094 ng/mL and recoveries over the range of 91.12 to 109.23% with CV < 8.8%.…”