A chemical determination of plasma inorganic iodine by ultrafiltration

Postmes, Th. J.; Coenegracht, J.M.

doi:10.1016/0009-8981(72)90121-0

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…Early methods for the determination of iodide in serum and urine were either not free of interference, such as the measurement of total iodine after ultrafiltration (Postmes andCoenegracht 1972, Globel 1977) and the use of iodide selective electrodes (Cooper and Croxon 1983), or were not suited for routine laboratory analysis, such as 131 I labelled serum (Makowetz et al 1966, Muller 1967) and neutron activation analysis (Aabech and Steinnes 1980). More recently, methods describing the selective determination of free I − in serum, plasma and urine, in the presence of organic iodine containing compounds, have been reported (Buchberger and Winsauer 1985, Buchberger and Huebauer 1989, Michalke et al 1996.…”

Section: Physiologicalmentioning

confidence: 99%

Optical halide sensing using fluorescence quenching: theory, simulations and applications - a review

Geddes¹

2001

Meas. Sci. Technol.

253

247

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In the last century the production and application of halides assumed an ever greater importance. In the fields of medicine, dentistry, plastics, pesticides, food, photography etc many new halogen containing compounds have come into everyday use. In an analogous manner many techniques for the detection and determination of halogen compounds and ions have been developed with scientific journals reporting ever more sensitive methods. The 19th century saw the discovery of what is now thought of as a classical method for halide determination, namely the quenching of fluorescence. However, little analytically was done until over 100 years after its discovery, when the first halide sensors based on the quenching of fluorescence started to emerge. Due to the typical complexity of fluorescence quenching kinetics of optical halide sensors and their lack of selectivity, they have found little if any place commercially, despite their sensitivity, where other techniques such as ion-selective electrodes, x-ray fluorescence spectroscopy and colorimetry have dominated the analytical market. In this review article the author summarizes the relevant theory and work to date for halide sensing using fluorescence quenching methods and outlines the future potential that fluorescence quenching based optical sensors have to offer in halide determination.

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Section: Physiologicalmentioning

confidence: 99%

Optical halide sensing using fluorescence quenching: theory, simulations and applications - a review

Geddes¹

2001

Meas. Sci. Technol.

253

247

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“…According to Stanley (Stanley, 1949) the calculation of serum inorganic iodide (Sil) is based on the assumption that the specific activity of SIT equals the specific activity of urinary inorganic iodide. Applying a simplified procedure with one blood sample only SIT can therefore be determined from the following formula (Stanley, 1949;Postmes and Coenegracht, 1972;Koutras et al, 1960): SIl = Serum 1271 = Serumt23I(lhr) X (Urinary'271(l-2hr) I Urinary i23I(1..2)) where 127J is stable iodine and 123J is a radioactive isotope of iodine frequently used in thyroid scintigraphy.…”

Section: Hplc Analysismentioning

confidence: 99%

“…Especially in iodine deficient areas levels of Sil significantly below 0.04 imol/L are usually found (Koutras et al, 1960;Alexander et al, 1962;Fitting, 1960). Furthermore direct methods for the chemical determination of Sil (Aumont and Tressol, 1987;Postmes and Coenegracht, 1972;Mantzos and Malamos, 1968) use the colorimetric ceric-arsenic assays which are based on the catalytic effect of iodide in the Sandell and Kolthoff reaction (Sandell and Kolthoff, 1937). These methods however are subject to various potential sources of error, mainly due to the fact, that even slight contaminations from the PBI (protein bound iodine) fraction or from free thyroid hormones may greatly increase the SIT value.…”

Section: Introductionmentioning

confidence: 99%

Methods for measuring iodine in urine and serum

Rendl¹,

Bier²,

Reiners³

2009

Exp Clin Endocrinol Diabetes

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The most important information in the determination of the status of iodine nutrition of a population comes from the measurement of the urinary excretion of iodine. Several methods are available for measuring urinary iodine. The choice among methods depends on the intended application, the number of samples, the cost and the technical capability. Epidemiological field studies demand simple, rapid and cost-effective methods. Suitable for these applications are the rapid urinary iodide test and the ammonium persulfate oxidation method which gives comparable results to the chloric acid method without having the drawbacks of being hazardous and explosive. In research studies however, sophisticated automated technology like the Technicon Autoanalyzer or Paired-Ion Reversed Phase HPLC are used in which the high cost of instrumentation are outweighed by the benefits of processing a large number of samples with high accuracy and minimal technician time. For determining serum inorganic iodide (SII) the HPLC assay is the method of choice, because contaminations from the protein bound iodine fraction do not interfere with the detection process. The clinical relevance of the measurement of SII is limited, but allows the calculation of the absolute iodine uptake which has great value in pathophysiologic studies.

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“…This technique has previously been used for thyroid hormones (10), various ions (11,12), and also cortisol (13)(14)(15)(16)(17). However, the ultrafiltration assays of unbound cortisol previously described necessitated large volumes of plasma (10-15 ml) which made them difficult to use for clinical purpose.…”

mentioning

confidence: 97%

Assay of Unbound Cortisol in Plasma*

Robin

Predine

Milgröm

1978

The Journal of Clinical Endocrinology & Metabolism

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A method for measuring the unbound fraction of plasma cortisol which is suitable for clinical use is described. It is shown that unless some precise experimental conditions are fulfilled, important errors may result when determining the unbound fraction of the hormone. The assay is practical (2 ml of plasma are necessary) and reproducible (coefficient of variation: 2.7% within the same assay and 3.1% in different assays). Unbound cortisol measured at 0800 h in 86 healthy individuals was 9.7 +/- 2.6% (SD) of total cortisol and 15.0 +/- 8.5 ng/ml of plasma (temperature: 37 C). No significant difference was found between men and women or according to age. In most cases, variations of unbound plasma cortisol were more important than the variations of total plasma cortisol. This explains why unbound cortisol was a better discriminator in some pathological conditions. In Cushing's syndrome unbound cortisol was found to be increased on the average 2.9 fold whereas total cortisol was only increased by 53%. Unbound cortisol was especially high (24.7 +/- 3.8% of total cortisol and 78 +/- 18.5 ng/ml of plasma) in Cushing's syndrome due to adrenal carcinoma. In adrenal insufficiency, unbound cortisol averaged 6% of total cortisol and 1.4 ng/ml of plasma.

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A chemical determination of plasma inorganic iodine by ultrafiltration

Cited by 7 publications

References 6 publications

Optical halide sensing using fluorescence quenching: theory, simulations and applications - a review

Optical halide sensing using fluorescence quenching: theory, simulations and applications - a review

Methods for measuring iodine in urine and serum

Assay of Unbound Cortisol in Plasma*

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