A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants

Маренкова, Т. В.; Дейнеко, Е. В.

doi:10.1134/s1022795406050085

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…Several copies of the transgene integration into the genome of transformed cell can lead to epigenetic transgene inactivation. Whereas, the increased transgene activity was observed in the presence of two or more transgene copies (Schubert et al 2004;Marenkova and Deineko 2006). However, a low number of transgene copies in a plant chromosome showed lower incidence of instability (Jones 2005).…”

Section: Discussionmentioning

confidence: 95%

Deciphering the role of fungal calmodulin gene using Host-mediated gene silencing approach in apple

Verma¹,

Modgil²,

Chauhan³

2021

Preprint

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Premature leaf fall caused by Marssonina coronaria is one of the most destructive diseases of apple in India. In this study, host induced gene silencing approach was exploited to develop resistance to this disease in an apple cultivar ‘Red Chief’. Calmodulin gene (CaM) having its role in fungal differentiation, development and pathogenicity was selected as target. hpRNAi construct was prepared from the conserved off target free partial gene sequence of CaM and used for transformation trials. Upto 6% kanamycin resistant shoots were obtained on selective medium having 5–6 mg/l kan after 7 weeks of coculture. In PCR analysis of 13 RNAi putative transformants, 10 lines were found positive with CaM and nptII gene specific primers and six lines showed hybridization signal. Semi qRT-PCR revealed variable levels of transgene expression among RNAi lines which seems to be related to copy number of integrated gene. In vitro detached leaf assay revealed lesion development and disease progression in wild type after 5 dpi but not visible in five CaM RNAi lines. Microscopic examination of infected control leaves showed fully developed, septate mycelium, and conidia along with necrosis of whole tissue while three transformants showed reduced growth and differentiation of fungus and in rest three, hyphal development and necrosis were strongly restricted. We conclude that trafficking of dsRNA/ siRNA from apple plants to pathogen might have triggered the down regulation of fungal CaM gene which confirms that deciphering the role of CaM through HIGS lead to resistance to Marssonina blotch in apple.

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Section: Discussionmentioning

confidence: 95%

Deciphering the role of fungal calmodulin gene using Host-mediated gene silencing approach in apple

Verma¹,

Modgil²,

Chauhan³

2021

Preprint

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show abstract

“…Several authors reported a positive correlation whereas other authors found no or negative correlation (see Schubert et al 2004). Recently, detailed studies were carried out which showed that the presence of two or more transgene copies can promote an increase in the level of transgene activity (Schubert et al 2004;Marenkova and Deineko, 2006). Down-regulation and silencing is expected if the copy number of the introduced genes exceeded a gene-specific threshold (Schubert et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants

Flachowsky¹,

Riedel²,

Reim³

et al. 2008

Electron. J. Biotechnol.

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The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and gene expression, we studied 26 transgenic apple lines carrying the attacin E

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Peculiarities of uidA Gene Expression under the Control of AP3 and RPT2a Tissue-Specific Gene Promotors of Arabidopsis thaliana L. in Nicotiana tabacum L. Transgenic Plants

Sidorchuk

Маренкова

Kuznetsov

et al. 2021

Russ J Plant Physiol

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A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants

Cited by 7 publications

References 23 publications

Deciphering the role of fungal calmodulin gene using Host-mediated gene silencing approach in apple

Deciphering the role of fungal calmodulin gene using Host-mediated gene silencing approach in apple

Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants

Peculiarities of uidA Gene Expression under the Control of AP3 and RPT2a Tissue-Specific Gene Promotors of Arabidopsis thaliana L. in Nicotiana tabacum L. Transgenic Plants

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