A cereal centromeric sequence

Aragón-Alcaide, Luis; Miller, Terry E.; Schwarzacher, Trude; Reader, S. M.; Moore, Graham

doi:10.1007/s004120050183

Cited by 66 publications

(88 citation statements)

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“…taushii, and rye, strong signals were observed mainly at the centromeric regions, in addition to very weak and dispersed signals on the arms ( Figure 3F-H). In addition, the centromeres were observed clustering at one pole and forming a ring-like allocation during the period before metaphase in the diploid species ( Figure 3D, E), which has been described in cells at interphase by Aragón-Alcaide et al (1996) and Wako et al (2003). Because the A-genome was included in the two tetraploid species (T. dicoccoides AABB and T. araraticum AAGG), distinct signals distributed along the arms were, not surprisingly, observed on the A-genome chromosomes in addition to strong signals on the centromeres and/or paracentromeres of all 28 chromosomes.…”

Section: Tbbac5 Fish Analysis Among Different Triticeae Speciessupporting

confidence: 73%

“…With the 32 RCS1-related BAC clones as templates, PCR was performed with specific primer sets for CCS1a (5'-TGCATCTATATTCTTGCTTGTT-3') and CCS1b (5'-GGTGCCCGATCTTTCGATGAGA-3') as described by Aragón-Alcaide et al (1996). The procedure was performed as follows: predenaturation for 3 min at 94 °C, 35 cycles of denaturation for 30 s at 94 °C,annealing for 1 min at 56 °C, extension for 90 s at 72°C…”

Section: Pcr Assay and Sequencingmentioning

confidence: 99%

“…One is the CCS1 family (Aragón-Alcaide et al 1996), and the other is the pSau3A9 family (Jiang et al 1996). Both have been detected in the centromeres of wheat, barley, rye, maize and rice (Aragón-Alcaide et al 1996;Jiang et al 1996;Presting et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Preliminary Analysis of Several Centromere‐associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library

Liu¹,

Yue²,

Dong³

et al. 2006

JIPB

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Although the centromeres of some plants have been investigated previously, our knowledge of the wheat centromere is still very limited. To understand the structure and function of the wheat centromere, we used two centromeric repeats (RCS1 and CCS1-5ab) to obtain some centromere-associated bacterial artificial chromosome (BAC) clones in 32 RCS1-related BAC clones that had been screened out from a diploid wheat (Triticum boeoticum Boiss.; 2n=2x=14) BAC library. Southern hybridization results indicated that, of the 32 candidates, there were 28 RCS1-positive clones. Based on gel blot patterns, the frequency of RCS1 was approximately one copy every 69.4 kb in these 28 RCS1-positive BAC clones. More bands were detected when the same filter was probed with CCS1-5ab. Furthermore, the CCS1 bands covered all the bands detected by RCS1, which suggests that some CCS1 repeats were distributed together with RCS1. The frequency of CCS1 families was once every 35.8 kb, nearly twice that of RCS1. Fluorescence in situ hybridization (FISH) analysis indicated that the five BAC clones containing RCS1 and CCS1 sequences all detected signals at the centromeric regions in hexaploid wheat, but the signal intensities on the A-genome chromosomes were stronger than those on the B-and/or Dgenome chromosomes. The FISH analysis among nine Triticeae cereals indicated that there were A-genomespecific (or rich) sequences dispersing on chromosome arms in the BAC clone TbBAC5. In addition, at the interphase cells, the centromeres of diploid species usually clustered at one pole and formed a ring-like allocation in the period before metaphase. Centromeres play critical roles in the faithful segregation of sister chromatids during the period of mitosis and meiosis (Presting et al. 1998;Jin et al. 2004). Although they have the same function, the DNA sequences of centromeric regions are not conserved among species (Clarke 1990;Houben and Schubert 2003;Ito et al. 2004;Jin et al. 2004). Owing to highly heterochromatic structure and complex repeats, it is more difficult to clone, map, sequence, and assemble the centromeres than the low-copy number DNA. Therefore, the centromere has become the last barrier of entire genome sequencing at present (Zhang et al. 2004b).In plants, considerable achievements have been made with regard to centromeres of model plants over the past few years. Highly repetitive "satellite" arrays and dispersed transponsable elements have been detected in most centromeres studied, where the former is organized into long stretches, interrupted by the latter and located at the core region . Studies have shown that the centromeres of Arabidopsis thaliana consist of a 178 ± 1 bp pAL1 satellite and Athila retroelement (Copenhaver et al. 1999;Houben and Schubert 2003). In rice (Oryza sativa L.), the DNA sequences in centromeres are composed of 155/156 bp CentO satellite and Centromeric Retrotransposon of BAC Clones Associated with Wheat Centromeres 349 Rice (CRR; Dong et al. 1998;Cheng et al. 2002;Jiang et al. 2003;Zhang et al. 20...

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Section: Tbbac5 Fish Analysis Among Different Triticeae Speciessupporting

confidence: 73%

Section: Pcr Assay and Sequencingmentioning

confidence: 99%

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Identification and Preliminary Analysis of Several Centromere‐associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library

Liu¹,

Yue²,

Dong³

et al. 2006

JIPB

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“…It accurately marks centromeres and delimits chromosome arms. The sequence was amplified by PCR in the presence of digoxigenin-11-dUTP (Roche) according to Aragon-Alcaide et al (1996). 25S rDNA is a 2.3-kb subclone of the 25S rDNA coding region of A. thaliana (Unfried and Gruendler 1990).…”

Section: Methodsmentioning

confidence: 99%

Molecular Assembly of Meiotic Proteins Asy1 and Zyp1 and Pairing Promiscuity in Rye (Secale cereale L.) and Its Synaptic Mutant sy10

et al. 2006

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Assembly of two orthologous proteins associated with meiotic chromosome axes in Arabidopsis thaliana (Asy1 and Zyp1) was studied immunologically at meiotic prophase of meiosis of wild-type rye (Secale cereale) and its synaptic mutant sy10, using antibodies derived from A. thaliana. The temporal and spatial expression of the two proteins were similar in wild-type rye, but with one notable difference. Unlike A. thaliana, in which foci of the transverse filament protein Zyp1 appear to linearize commensurately with synapsis, linear tracts of Asy1 and Zyp1 protein form independently at leptotene and early zygotene of rye and coalign into triple structures resembling synaptonemal complexes (SCs) only at later stages of synapsis. The sy10 mutant used in this study also forms spatially separate linear tracts of Asy1 and Zyp1 proteins at leptotene and early zygotene, and these coalign but do not form regular triple structures at midprophase. Electron microscopy of spread axial elements reveals extensive asynapsis with some exchanges of pairing partners. Indiscriminate SCs support nonhomologous chiasma formation at metaphase I, as revealed by multi-color fluorescence in situ hybridization enabling reliable identification of all the chromosomes of the complement. Scrutiny of chiasmate associations of chromosomes at this stage revealed some specificity in the associations of homologous and nonhomologous chromosomes. Inferences about the nature of synapsis in this mutant were drawn from such observations. T HE availability of advanced genomic and proteomic resources in tractable model organisms, such as yeast and Arabidopsis thaliana, has provided unprecedented access to the genes and proteins involved in the control of meiosis. This functional genomic infrastructure has also precipitated detailed comparisons of meiosis in closely and distantly related organisms, not only with the intention of isolating orthologs with key roles in the process, but also with the goal of assaying the degree of similarity of structure and function of key meiotic genes and proteins of organisms across the phylogenetic spectrum. Such comparisons have revealed that meiosis is conserved, insofar as a number of meiotic genes appear to have orthologs in a range of different organisms (for reviews see Zickler and

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“…They note the presence of a CENP-B binding site in the alphoid repeat as well as in certain transposable elements. A consensus binding site for CENP-B also exists in the grass repeat isolated by Aragon-Alcaide et al (1996), in the maize neocentromere repeat (T. Phelps, pers. comm.…”

Section: Inhibition Of Recombination By Centromeresmentioning

confidence: 99%

Do These Sequences Make CENs Yet?

Birchler¹

1997

Genome Res.

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A cereal centromeric sequence

Cited by 66 publications

References 0 publications

Identification and Preliminary Analysis of Several Centromere‐associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library

Identification and Preliminary Analysis of Several Centromere‐associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library

Molecular Assembly of Meiotic Proteins Asy1 and Zyp1 and Pairing Promiscuity in Rye (Secale cereale L.) and Its Synaptic Mutant sy10

Do These Sequences Make CENs Yet?

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