A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris

Chen, Hongxin; Quintana, Julia; Kovalchuk, Andriy; Ubhayasekera, Wimal; Asiegbu, Fred O.

doi:10.1016/j.fgb.2015.09.007

Cited by 25 publications

(22 citation statements)

References 90 publications

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“…After treating with a washing buffer (20 mM Na 3 PO 4 , 0.5 M NaCl, 50 mM iminazole, pH 7.3), the protein was eluted by an elution buffer (20 mM Na 3 PO 4 , 0.5 M NaCl, 500 mM iminazole, pH 7.3), and concentrated by an ultrafiltration tube. The protein solution containing CmCP was confirmed by Western blot (Chen et al, 2015), which was stored at 80 • C for later use. Western blot was performed using anti-c-myc antibody produced in rabbit (Sigma, Beijing, China).…”

Section: Expression Of Cmcp In Pichia Pastorismentioning

confidence: 99%

Characterization of cmcp Gene as a Pathogenicity Factor of Ceratocystis manginecans

Zhang

Luo

et al. 2020

Front. Microbiol.

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Ceratocystis manginecans causes mango wilt with significant economic losses. In the infection court, cerato-platanin (CP) family proteins (CPPs) are believed to involve in pathogenesis but has not been determined in C. manginecans. To confirm this function, a CP protein (CmCP) of C. manginecans was characterized in this study. A protoplast of C. manginecans was prepared by treating its mycelia with driselase and lysing enzymes. The cmcp gene was edited using CRISPR/Cas-U6-1 expression vectors in 60% PEG and 50 µg/mL hygromycin B in the medium, resulting in mutants with cmcp deletion (cmcp). A complemented mutant (cmcp-C) was obtained by transforming cmcp to cmcp. Both cmcp and cmcp-C were characterized by comparing them with a wild-type strain on morphology, mycelial growth, conidial production and pathogenicity. Additionally, cmcp was transformed and expressed in Pichia pastoris, and the derived recombinant protein CmCP caused a severe necrosis on Nicotiana tabacum leaves. CmCP-treated plant leaves showed symptoms of hypersensitive response including electrolyte leakage, reactive oxygen species generation and overexpression of defenserelated genes PR-1, PAD3, ERF1, HSR203J, and HIN1. All those results suggested that cmcp gene was required for the growth development of C. manginecans and functioned as a major pathogenicity factor in mango infection.

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Section: Expression Of Cmcp In Pichia Pastorismentioning

confidence: 99%

Characterization of cmcp Gene as a Pathogenicity Factor of Ceratocystis manginecans

Zhang

Luo

et al. 2020

Front. Microbiol.

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“…Till now, in addition to CP, 11 more CPP family CIDPs have been identified from different fungal pathogens ( Table 2 ), such as BcSpl1 in B. cinerea ( Frías et al, 2011 , 2013 ), EPL1 in Colletotrichum falcatum ( Ashwin et al, 2017 ), CgCP1 in Colletotrichum gloeosporioides ( Wang W. et al, 2018 ), Pop1 in Ceratocystis populicola ( Comparini et al, 2009 ), FgCPP2 in F. graminearum ( Quarantin et al, 2016 , 2019 ), FocCP1 in Fusarium oxysporum ( Li et al, 2019a ; Liu et al, 2019 ), HaCPL2 in Heterobasidion annosum sensu stricto ( Chen et al, 2015 ), MoSM1/MSP1 ( Jeong et al, 2007 ; Yang et al, 2009 ; Wang et al, 2016 ; Hong et al, 2017 ) in M. oryzae , MpCP1 in Moniliophthora perniciosa ( Zaparoli et al, 2009 ), SsCP1 in Sclerotinia sclerotiorum ( Yang et al, 2018b ), and VdCP1 in Verticillium dahliae ( Zhang et al, 2017a ). Similar to CP, these 11 CPP family CIDPs induced strong necrosis and elicited defense responses in plants.…”

Section: Major Protein Families Of Oomycete and Fungus Apoplastic CDImentioning

confidence: 99%

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

Han

et al. 2020

Front. Genet.

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Filamentous pathogens, such as phytopathogenic oomycetes and fungi, secrete a remarkable diversity of apoplastic effector proteins to facilitate infection, many of which are able to induce cell death in plants. Over the past decades, over 177 apoplastic cell death-inducing proteins (CDIPs) have been identified in filamentous oomycetes and fungi. An emerging number of studies have demonstrated the role of many apoplastic CDIPs as essential virulence factors. At the same time, apoplastic CDIPs have been documented to be recognized by plant cells as pathogen-associated molecular patterns (PAMPs). The recent findings of extracellular recognition of apoplastic CDIPs by plant leucine-rich repeat-receptor-like proteins (LRR-RLPs) have greatly advanced our understanding of how plants detect them and mount a defense response. This review summarizes the latest advances in identifying apoplastic CDIPs of plant pathogenic oomycetes and fungi, and our current understanding of the dual roles of apoplastic CDIPs in plant-filamentous pathogen interactions.

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“…Such further analysis would also allow investigation of the effector-mediated signalling among the three actors in the system. Interestingly, a cerato-platanin-like protein of H. annosum has been recently characterized as an effector inducing defence responses in Pinus sylvestris L. (Chen et al, 2015). In addition, it is known that colonization of roots by ECM fungi may involve the activation of genes coding for effectors that can overcome plant defences and that may resemble those of pathogenic fungi (Plett et al, 2011).…”

Section: Researchmentioning

confidence: 99%

A nonnative and a native fungal plant pathogen similarly stimulate ectomycorrhizal development but are perceived differently by a fungal symbiont

et al. 2016

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The effects of plant symbionts on host defence responses against pathogens have been extensively documented, but little is known about the impact of pathogens on the symbiosis and if such an impact may differ for nonnative and native pathogens. Here, this issue was addressed in a study of the model system comprising Pinus pinea, its ectomycorrhizal symbiont Tuber borchii, and the nonnative and native pathogens Heterobasidion irregulare and Heterobasidion annosum, respectively. In a 6-month inoculation experiment and using both in planta and gene expression analyses, we tested the hypothesis that H. irregulare has greater effects on the symbiosis than H. annosum. Although the two pathogens induced the same morphological reaction in the plant-symbiont complex, with mycorrhizal density increasing exponentially with pathogen colonization of the host, the number of target genes regulated in T. borchii in plants inoculated with the native pathogen (i.e. 67% of tested genes) was more than twice that in plants inoculated with the nonnative pathogen (i.e. 27% of genes). Although the two fungal pathogens did not differentially affect the amount of ectomycorrhizas, the fungal symbiont perceived their presence differently. The results may suggest that the symbiont has the ability to recognize a self/native and a nonself/nonnative pathogen, probably through host plant-mediated signal transduction.

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A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris

Cited by 25 publications

References 90 publications

Characterization of cmcp Gene as a Pathogenicity Factor of Ceratocystis manginecans

Characterization of cmcp Gene as a Pathogenicity Factor of Ceratocystis manginecans

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

A nonnative and a native fungal plant pathogen similarly stimulate ectomycorrhizal development but are perceived differently by a fungal symbiont

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