A centromere-specific probe for fluorescence in situ hybridization on chromosomes of Apis mellifera L

Beye, Martin; Moritz, Robin F. A.

doi:10.1051/apido:19940307

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…Due to the difficulties in interpreting banding patterns in honey bee chromosomes, the position of the centromeres is not well defined. Some evidence based on G- and C- banding suggests there are four metacentric and 12 submetacentric or subtelocentric chromosomes [70], whereas other evidence based on the fluorescent in situ hybridization of a centromere probe suggests there are two metacentric, four submetacentric, two subtelocentric and eight telocentric chromosomes [71]. Our evidence suggests at least six chromosomes that could be telocentric or acrocentric: chromosomes 3, 5, 6, 9, 14 and 15.…”

Section: Discussionmentioning

confidence: 55%

Complex population structure and haplotype patterns in Western Europe honey bee from sequencing a large panel of haploid drones

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Canale-Tabet

et al. 2021

Preprint

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Honey bee subspecies originate from specific geographic areas in Africa, Europe and the Middle East. The interest of beekeepers in specific phenotypes has led them to import subspecies to regions outside of their original range. The resulting admixture complicates population genetics analyses and populations stratification can be a major problem for association studies. As a typical example, the case of the French population is studied here. We sequenced 870 haploid drones for SNP detection and identified nine genetic backgrounds in 629 samples. Five correspond to subspecies, two to isolated populations and two to human-mediated population management. We also highlight several large haplotype blocks, some of which coinciding with the position of centromeres. The largest is 3.6 Mb long on chromosome 11, representing 1.6 % of the genome and has two major haplotypes, corresponding to the two dominant genetic background identified.

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Section: Discussionmentioning

confidence: 55%

Complex population structure and haplotype patterns in Western Europe honey bee from sequencing a large panel of haploid drones

Wragg

Basso

Canale-Tabet

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Due to the difficulties in interpreting banding patterns in honey bee chromosomes, the position of the centromeres is not well defined. Some evidence based on G‐ and C‐banding suggests there are four metacentric and 12 submetacentric or subtelocentric chromosomes (Hoshiba, 1984 ), whereas other evidence based on fluorescence in situ hybridization of a centromere probe suggests there are two metacentric, four submetacentric, two subtelocentric and eight telocentric chromosomes (Beye & Moritz, 1994 ). Our evidence suggests at least six chromosomes that could be telocentric or acrocentric: chromosomes 3, 5, 6, 9, 14 and 15.…”

Section: Discussionmentioning

confidence: 99%

Complex population structure and haplotype patterns in the Western European honey bee from sequencing a large panel of haploid drones

Wragg

Basso

Canale-Tabet

et al. 2022

Molecular Ecology Resources

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Honey bee subspecies originate from specific geographical areas in Africa, Europe and the Middle East, and beekeepers interested in specific phenotypes have imported genetic material to regions outside of the bees' original range for use either in pure lines or controlled crosses. Moreover, imported drones are present in the environment and mate naturally with queens from the local subspecies. The resulting admixture complicates population genetics analyses, and population stratification can be a major problem for association studies. To better understand Western European honey bee populations, we produced a whole genome sequence and single nucleotide polymorphism (SNP) genotype data set from 870 haploid drones and demonstrate its utility for the identification of nine genetic backgrounds and various degrees of admixture in a subset of 629 samples. Five backgrounds identified correspond to subspecies, two to isolated populations on islands and two to managed populations. We also highlight several large haplotype blocks, some of which coincide with the position of centromeres. The largest is 3.6 Mb long and represents 21% of chromosome 11, with two major haplotypes corresponding to the two dominant genetic backgrounds identified. This large naturally phased data set is available as a single vcf file that can now serve as a reference for subsequent populations genomics studies in the honey bee, such as (i) selecting individuals of verified homogeneous genetic backgrounds as references, (ii) imputing genotypes from a lower‐density data set generated by an SNP‐chip or by low‐pass sequencing, or (iii) selecting SNPs compatible with the requirements of genotyping chips.

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Sex linkage in the honeybeeApis mellifera detected by multilocus DNA fingerprinting

Beye

Moritz

Epplen

1994

Naturwissenschaften

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A centromere-specific probe for fluorescence in situ hybridization on chromosomes of Apis mellifera L

Cited by 5 publications

References 9 publications

Complex population structure and haplotype patterns in Western Europe honey bee from sequencing a large panel of haploid drones

Complex population structure and haplotype patterns in Western Europe honey bee from sequencing a large panel of haploid drones

Complex population structure and haplotype patterns in the Western European honey bee from sequencing a large panel of haploid drones

Sex linkage in the honeybeeApis mellifera detected by multilocus DNA fingerprinting

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