A Centrin3-dependent, Transient, Appendage of the Mother Basal Body Guides the Positioning of the Daughter Basal Body in Paramecium

Jerka‐Dziadosz, Maria; Włoga, Dorota; Gogendeau, Delphine; Loubresse, N. Garreau de; Ruiz, Françoise; Fabczak, S.; Beisson, Janine

doi:10.1016/j.protis.2012.11.003

Cited by 25 publications

(35 citation statements)

References 92 publications

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“…We then compared the time of appearance of new ICL1e dots to that of SAS-6, the earliest known marker of nascent basal bodies in Paramecium (Jerka-Dziadosz et al, 2012). In cells expressing both RFP-SAS-6 and GFP-ICL1e, the SAS-6 and ICL1e foci co-localise at all new single basal bodies (Fig.…”

Section: Resultsmentioning

confidence: 99%

Centrin diversity and basal body patterning across evolution: new insights fromParamecium

et al. 2017

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First discovered in unicellular eukaryotes, centrins play crucial roles in basal body duplication and anchoring mechanisms. While the evolutionary status of the founding members of the family, Centrin2/Vfl2 and Centrin3/cdc31 has long been investigated, the evolutionary origin of other members of the family has received less attention. Using a phylogeny of ciliate centrins, we identify two other centrin families, the ciliary centrins and the centrins present in the contractile filaments (ICL centrins). In this paper, we carry on the functional analysis of still not well-known centrins, the ICL1e subfamily identified in Paramecium, and show their requirement for correct basal body anchoring through interactions with Centrin2 and Centrin3. Using Paramecium as well as a eukaryote-wide sampling of centrins from completely sequenced genomes, we revisited the evolutionary story of centrins. Their phylogeny shows that the centrins associated with the ciliate contractile filaments are widespread in eukaryotic lineages and could be as ancient as Centrin2 and Centrin3.

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Section: Resultsmentioning

confidence: 99%

Centrin diversity and basal body patterning across evolution: new insights fromParamecium

et al. 2017

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“…This phenotype is similar to that reported for the knockdown of PtCEN3 (Ruiz et al, 2005), and for its homologue, Tetrahymena CEN2 (Vonderfecht et al, 2012), suggesting that Sfr13 and Cen2 may directly interact with each other. One intriguing possibility is that Sfr13 plays a role in the assembly or function of the newly identified anterior left filament (ALF), which is present at assembling basal bodies, requires PtCEN3 function in Paramecium, and may play a role in daughter basal body positioning (Jerka-Dziadosz et al, 2012). Some centrins and Sfi1 repeat proteins have been found in calcium responsive contractile structures, notably in the infraciliary lattice in Paramecium (Gogendeau et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Sfr13 is a member of a large family of asymmetrically 1 localized Sfi1-repeat proteins and is important for basal body separation and stability inTetrahymena thermophila

Stemm-Wolf

Meehl

Winey

2013

Journal of Cell Science

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SummaryDirected fluid flow, which is achieved by the coordinated beating of motile cilia, is required for processes as diverse as cellular swimming, developmental patterning and mucus clearance. Cilia are nucleated, anchored and aligned at the plasma membrane by basal bodies, which are cylindrical microtubule-based structures with ninefold radial symmetry. In the unicellular ciliate Tetrahymena thermophila, two centrin family members associated with the basal body are important for both basal body organization and stabilization. We have identified a family of 13 proteins in Tetrahymena that contain centrin-binding repeats related to those identified in the Saccharomyces cerevisiae Sfi1 protein. We have named these proteins Sfr1-Sfr13 (for Sfi1-repeat). Nine of the Sfr proteins localize in unique polarized patterns surrounding the basal body, suggesting non-identical roles in basal body organization and association with basal body accessory structures. Furthermore, the Sfr proteins are found in distinct basal body populations in Tetrahymena cells, indicating that they are responsive to particular developmental programs. A complete genetic deletion of one of the family members, Sfr13, causes unstable basal bodies and defects in daughter basal body separation from the mother, phenotypes also observed with centrin disruption. It is likely that the other Sfr family members are involved in distinct centrin functions, providing specificity to the tasks that centrins perform at basal bodies.

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“…The formation of the ALF structure coincides with new basal body assembly and had thus been suggested to promote the formation of new basal bodies (Jerka-Dziadosz et al, 2013). However, it appears that the primary function of ALF is to position basal bodies near the cell cortex (Jerka-Dziadosz et al, 2013) after their assembly.…”

Section: Basal Body Biogenesis Within the Local Environmentmentioning

confidence: 99%

“…For instance, the anterior left filament (ALF) (Fig. 2B-D) is a transient appendage that appears coincident with new basal body assembly and then disappears once the basal body docks at the cell cortex (Jerka-Dziadosz et al, 2013). ALF formation in Paramecium requires centrin-3, which is necessary for basal body positioning at the cortex.…”

Section: Asymmetric Basal Body Biogenesis a Defined Site Of New Basalmentioning

confidence: 99%

Choosing sides – asymmetric centriole and basal body assembly

Pearson

2014

Journal of Cell Science

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Centrioles and basal bodies (CBBs) are microtubule-rich cylindrical structures that nucleate and organize centrosomes and cilia, respectively. Despite their apparent ninefold rotational symmetry, the nine sets of triplet microtubules in CBBs possess asymmetries in their morphology and in the structures that associate with them. These asymmetries define the position of nascent CBB assembly, the orientation of ciliary beating, the orientation of spindle poles and the maintenance of cellular geometry. For some of these functions, the orientation of CBBs is first established during new CBB biogenesis when the daughter structure is positioned adjacent to the mother. The mother CBB organizes the surrounding environment that nascent CBBs are born into, thereby providing a nest for the new CBB to develop. Protists, including ciliates and algae, highlight the importance of this environment with the formation of asymmetrically placed scaffolds onto which new basal bodies assemble and are positioned. Recent studies illuminate the positioning of nascent centrioles relative to a modular pericentriolar material (PCM) environment and suggest that, like ciliates, centrosomes organize an immediate environment surrounding centrioles for their biogenesis and positioning. In this Commentary, I will explore the positioning of nascent CBB assembly as the first event in building cellular asymmetries and describe how the environment surrounding both basal bodies and centrioles may define asymmetric assembly.

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A Centrin3-dependent, Transient, Appendage of the Mother Basal Body Guides the Positioning of the Daughter Basal Body in Paramecium

Cited by 25 publications

References 92 publications

Centrin diversity and basal body patterning across evolution: new insights fromParamecium

Centrin diversity and basal body patterning across evolution: new insights fromParamecium

Sfr13 is a member of a large family of asymmetrically 1 localized Sfi1-repeat proteins and is important for basal body separation and stability inTetrahymena thermophila

Choosing sides – asymmetric centriole and basal body assembly

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