A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Moen, Scott T.; Hatcher, Christopher L.; Singh, Anup K.

doi:10.1371/journal.pone.0153137

Cited by 23 publications

(19 citation statements)

References 24 publications

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“…After incubation was done, 5 mL of reaction volume (in 3 replicas) were dispensed into wells on a disk and the disk was spun at 5000 rpm for 45 sec. A more detailed assay design is explained elsewhere [36].…”

Section: Assay Designmentioning

confidence: 99%

Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples

Litvinov¹,

St²,

Gj³

et al. 2017

J Bioengineer & Biomedical Sci

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Infection with Mycobacterium Tuberculosis represents a significant threat to people with immune disorders, such as HIV-positive individuals, and can result in significant health complications or death if not diagnosed and treated early. We present a centrifugal microfluidic platform for multiplexed detection of tuberculosis and HIV biomarkers in human whole blood with minimal sample preparation and a sample-to-answer time of 30 minutes. This multiplexed assay was developed for the detection of two M.tuberculosis secreted proteins, whose secretion represents an active and ongoing infection, as well as detection of HIV p24 protein and human anti-p24 antibodies. The limit of detection for this multiplex assay is in the pg/mL range for both HIV and M.tuberculosis proteins, making this assay potentially useful in the clinical diagnosis of both HIV and Tuberculosis proteins indicative of active infection. Antigen detection for the HIV assay sensitivity was 89%, the specificity 85%. Serological detection had 100% sensitivity and specificity for the limited sample pool. The centrifugal microfluidic platform presented here offers the potential for a portable, fast and inexpensive multiplexed diagnostic device that can be used in resource-limited settings for diagnosis of TB and HIV.

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Section: Assay Designmentioning

confidence: 99%

Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples

Litvinov¹,

St²,

Gj³

et al. 2017

J Bioengineer & Biomedical Sci

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show abstract

“…Recently, more and more attention has been drawn to selective separation of target cell populations from whole blood. A density gradient has been employed to separate WBCs from RBCs [65] or to enrich basophils [66] from peripheral human blood.…”

Section: Lab-on-a-diskmentioning

confidence: 99%

On-chip sample preparations for Point-of-Care cellular analysis of blood

Zhang¹,

Terstappen²,

Beck³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…Meticulous analytical methods especially those in medical fields often require a concentration step before any analysis is performed. Several methods to perform the concentration have been developed, including density‐gradient‐based centrifugation , fluorescent‐activated cell sorting , cell surface markers , and laser manipulation of particles .…”

Section: Introductionmentioning

confidence: 99%

A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

2017

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In recent years, applications of dielectrophoresis-based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast, Escherichia coli, or latex particles. A chemical deposition-based method was employed for fabrication of microelectrodes, inducing nonuniform electric field required for dielectrophoresis-based separation. One major advantage of our employed method is low fabrication cost, in addition to its accuracy and operation at low voltages. The performance of the microfluidic device in enriching either of injected samples was studied using spectrophotometric techniques. The effects of experimentally controllable parameters (applied-voltage amplitude and frequency, and flow rate) were studied by changing a parameter while keeping the others constant. It became evident that all the aforementioned parameters had modulating impact on the performance of the microfluidic device. Furthermore, to investigate binary interactions among the parameters, response surface methodology was exploited, resulting in a second-order polynomial model for the performance of the device as a function of the parameters. The model was employed for finding the optimum values of the parameters at which the performance of the device is the highest. At optimum values for the experimentally controllable parameters, enrichment efficiencies of 87 ± 2, 82 ± 4, and 86 ± 3% for, respectively, yeast, E. coli, and latex particles were obtained experimentally, confirming the ability of the proposed method for biological and polymeric particles enrichment.

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A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Cited by 23 publications

References 24 publications

Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples

Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples

On-chip sample preparations for Point-of-Care cellular analysis of blood

A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

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