A cellulose-decomposing bacterial association

Думова, В. А.; Kruglov, Yu. V.

doi:10.1134/s0026261709020155

Cited by 19 publications

(15 citation statements)

References 12 publications

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“…DB13. A cellulosedecomposing bacterial association included Alcaligenes sp., Ochrobactrum sp., Sphingomonas sp., Achromobacter sp., Pseudomonas sp., and Sporocytophaga [59]. A study [60] reported xylanase production from Bacillus thermoleovorans strain K-3D and Bacillus flavothermus strain LB3A.…”

Section: Discussionmentioning

confidence: 97%

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Okeke

2010

Appl Biochem Biotechnol

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Diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. Inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. In an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, purified 19 monocultures and evaluated their cellulolytic-xylanolytic potential. Five selected isolates (DB1, DB2, DB7, DB8, and DB13) were used to compose a defined consortium and characterized by 16S ribosomal RNA gene sequence analysis. Nucleotide sequence blast analysis revealed that DB1, DB2, DB7, DB8, and DB13 were respectively similar to Pseudoxanthomonas byssovorax (99%), Microbacterium oxydans (99%), Bacillus sp. (99%), Ochrobactrum anthropi (98%), and Klebsiella trevisanii (99%). The isolates produced an array of cellulolytic-xylanolytic enzymes (filter paper cellulase, β-glucosidase, xylanase, and β-xylosidase), and significant activities were recorded in 30 min. Isolates DB1 and DB2 displayed the highest filter paper cellulase: 27.83 and 31.22 U mg⁻¹, respectively. The highest β-glucosidase activity (18.07 U mg⁻¹) was detected in the culture of isolate DB1. Isolate DB2 produced the highest xylanase activity (103.05 U mg⁻¹), while the highest β-xylosidase activity (7.72 U mg⁻¹) was observed with DB13. Use of microbial consortium in bioprocessing of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, adsorption, and requirement for high amounts of enzymes in direct use of enzymes.

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Section: Discussionmentioning

confidence: 97%

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Okeke

2010

Appl Biochem Biotechnol

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“…Several members of the family Enterobacteriaceae, genera Dietzia , Salinibacterium , Sphingomonas and Luteibacter , as well as some nitrogen-fixing representatives of the genera Agrobacterium and Rhizobium , comprised most the bacterial communities in TC larval guts (but depending on the individual larva). Sphingomonas and Luteibacter bacteria with different metabolic capabilities can destroy the cellulose 39 , 40 . The predicted genes for lignocellulose degradation and nitrogenase component proteins were also enriched in most of the TC larval guts (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparison of intestinal bacterial and fungal communities across various xylophagous beetle larvae (Coleoptera: Cerambycidae)

Mohammed

Ziganshina

Shagimardanova

et al. 2018

Sci Rep

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The microbial gut communities associated with various xylophagous beetles offer great potential for different biotechnologies and elaboration of novel pest management strategies. In this research, the intestinal bacterial and fungal communities of various cerambycid larvae, including Acmaeops septentrionis, Acanthocinus aedilis, Callidium coriaceum, Trichoferus campestris and Chlorophorus herbstii, were investigated. The intestinal microbial communities of these Cerambycidae species were mostly represented by members of the bacterial phyla Proteobacteria and Actinobacteria and the fungal phylum Ascomycota. However, the bacterial and fungal communities varied by beetle species and between individual organisms. Furthermore, bacterial communities’ metagenomes reconstruction indicated the genes that encode enzymes involved in the lignocellulose degradation (such as peroxidases, alpha-L-fucosidases, beta-xylosidases, beta-mannosidases, endoglucanases, beta-glucosidases and others) and nitrogen fixation (nitrogenases). Most of the predicted genes potentially related to lignocellulose degradation were enriched in the T. campestris, A. aedilis and A. septentrionis larval gut consortia, whereas predicted genes affiliated with the nitrogenase component proteins were enriched in the T. campestris, A. septentrionis and C. herbstii larval gut consortia. Several bacteria and fungi detected in the current work could be involved in the nutrition of beetle larvae.

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“…Application of carrot residue increased the relative abundance of bacterial groups that are known to be favoured by organic material application, such as the Actinomycetales, Acetobacteraceae (Alphaproteobacteria) and Pseudomonadaceae (Gammaproteobacteria). Strains belonging to the Pseudomonadaceae are known to degrade cellulose [ 43 ] and hemicellulose [ 44 ], so they were favoured by application of the carrot residue.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Bacterial Community Structure of Remediated Anthracene-Contaminated Soils

et al. 2016

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Mixing soil or adding earthworms (Eisenia fetida (Savigny, 1826)) accelerated the removal of anthracene, a polycyclic aromatic hydrocarbon, from a pasture and an arable soil, while a non-ionic surfactant (Surfynol® 485) inhibited the removal of the contaminant compared to the untreated soil. It was unclear if the treatments affected the soil bacterial community and consequently the removal of anthracene. Therefore, the bacterial community structure was monitored by means of 454 pyrosequencing of the 16S rRNA gene in the pasture and arable soil mixed weekly, amended with Surfynol® 485, E. fetida or organic material that served as food for the earthworms for 56 days. In both soils, the removal of anthracene was in the order: mixing soil weekly (100%) > earthworms applied (92%) > organic material applied (77%) > untreated soil (57%) > surfactant applied (34%) after 56 days. There was no clear link between removal of anthracene from soil and changes in the bacterial community structure. On the one hand, application of earthworms removed most of the contaminant from the arable soil and had a strong effect on the bacterial community structure, i.e. a decrease in the relative abundance of the Acidobacteria, Chloroflexi and Gemmatimonadetes, and an increase in that of the Proteobacteria compared to the unamended soil. Mixing the soil weekly removed all anthracene from the arable soil, but had little or no effect on the bacterial community structure. On the other hand, application of the surfactant inhibited the removal of anthracene from the arable soil compared to the untreated soil, but had a strong effect on the bacterial community structure, i.e. a decrease in the relative abundance of Cytophagia (Bacteroidetes), Chloroflexi, Gemmatimonadetes and Planctomycetes and an increase in that of the Flavobacteria (Bacteroidetes) and Proteobacteria. Additionally, the removal of anthracene was similar in the different treatments of both the arable and pasture soil, but the effect of application of carrot residue, earthworms or the surfactant on the bacterial community structure was more accentuated in the arable soil than in the pasture soil. It was found that removal of anthracene was not linked to changes in the bacterial community structure.

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A cellulose-decomposing bacterial association

Cited by 19 publications

References 12 publications

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Comparison of intestinal bacterial and fungal communities across various xylophagous beetle larvae (Coleoptera: Cerambycidae)

Changes in the Bacterial Community Structure of Remediated Anthracene-Contaminated Soils

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