“…By using an mRNA that bears both the HCV 59UTR and HCV 39UTR as an affinity matrix, we specifically purified proteins that have been shown to interact with either the HCV 59UTR or HCV 39UTR, or both. The proteins IGF2BP1, hnRNP C, hnRNP D, hnRNP Q, polypyrimidine tract binding protein 1, PAI-1 mRNA binding protein 1, nucleolin, NF90/NFAR, RNA helicase A, and five subunits of eIF 3 (out of nine identified) match with proteins isolated from cytoplasmic Huh7 cell extract employing an HCV 59UTR that lacks domain I and represents the HCV IRES consisting of domains II to IV (Kim et al 2004;Lu et al 2004). HnRNP C, YB-1, polypyrimidine tract binding protein 1, DDX3, NF90/NFAR, and RNA helicase A have been identified as HCV 39UTR binding proteins in Huh7 extract (Gontarek et al 1999;Harris et al 2006;Isken et al 2007).…”