A cellular mechanism underlying the restoration of thermo/photoperiod-sensitive genic male sterility

Shi, Qiang-Sheng; Lou, Yue; Shen, Shiyi; Wang, Shenghong; Zhou, Lei; Wang, Junjie; Liu, Xinglu; Xiong, Shuang-Xi; Yue-hong, Han; Zhou, Haisheng; Huang, Xuehui; Wang, Shui; Zhu, Jun; Yang, Zhong‐Nan

doi:10.1016/j.molp.2021.08.019

Cited by 29 publications

(27 citation statements)

References 47 publications

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“…It is reported to function in degrading the pollen mother cell wall during microspore development in Arabidopsis (Rhee et al ., 2003 ). The antibody against de‐esterified pectin stained the primexine during the early microspore stage of pollen development in both the wild type and the qrt3‐1 mutant in Arabidopsis (Rhee et al ., 2003 ), and recent study showed that the AtQRT3 delayed degradation of the tetrad pectin wall (Shi et al ., 2021 ). Moreover, exine phenotypes of qrt‐dex1 and qrt‐dex2 indicated that the polygalacturonase activity of AtQRT3 may be important for proper exine patterning (Dobritsa et al ., 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Degradation of de‐esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton

Xiao

et al. 2022

Plant Biotechnology Journal

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Summary The pollen wall exine provides a protective layer for the male gametophyte and is largely composed of sporopollenin, which comprises fatty acid derivatives and phenolics. However, the biochemical nature of the external exine is poorly understood. Here, we show that the male sterile line 1355A of cotton mutated in NO SPINE POLLEN (GhNSP) leads to defective exine formation. The GhNSP locus was identified through map‐based cloning and confirmed by genetic analysis (co‐segregation test and allele prediction using the CRISPR/Cas9 system). In situ hybridization showed that GhNSP is highly expressed in tapetum. GhNSP encodes a polygalacturonase protein homologous to AtQRT3, which suggests a function for polygalacturonase in pollen exine formation. These results indicate that GhNSP is functionally different from AtQRT3, the latter has the function of microspore separation. Biochemical analysis showed that the percentage of de‐esterified pectin was significantly increased in the 1355A anthers at developmental stage 8. Furthermore, immunofluorescence studies using antibodies to the de‐esterified and esterified homogalacturonan (JIM5 and JIM7) showed that the Ghnsp mutant exhibits abundant of de‐esterified homogalacturonan in the tapetum and exine, coupled with defective exine formation. The characterization of GhNSP provides new understanding of the role of polygalacturonase and de‐esterified homogalacturonan in pollen exine formation.

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Section: Discussionmentioning

confidence: 99%

Degradation of de‐esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton

Xiao

et al. 2022

Plant Biotechnology Journal

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“…However, the PG gene family in maize has not been extensively studied, including their function in controlling anther development. Previous work in Arabidopsis has revealed that several PGs are critical for male gamete development, and we assume some maize PGs should also play a similar role as in Arabidopsis [18,42]. Identifying and characterizing PGs from maize will provide potential gene resources for hybrid seed production using different breeding systems such as the multi-control sterility system [43].…”

Section: Introductionmentioning

confidence: 94%

Genome-Wide Identification and Characterization of Polygalacturonase Gene Family in Maize (Zea mays L.)

Hou

Wang

et al. 2021

IJMS

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Polygalacturonase (PG, EC 3.2.1.15) is a crucial enzyme for pectin degradation and is involved in various developmental processes such as fruit ripening, pollen development, cell expansion, and organ abscission. However, information on the PG gene family in the maize (Zea mays L.) genome and the specific members involved in maize anther development are still lacking. In this study, we identified 55 PG family genes from the maize genome and further characterized their evolutionary relationship and expression patterns. Phylogenetic analysis revealed that ZmPGs are grouped into six Clades, and gene structures of the same Clade are highly conserved, suggesting their functional conservation. The ZmPGs are randomly distributed across maize chromosomes, and collinearity analysis showed that many ZmPGs might be derived from tandem duplications and segmental duplications, and these genes are under purifying selection. Furthermore, gene expression analysis provided insights into possible functional divergence among ZmPGs. Based on the RNA-seq data analysis, we found that many ZmPGs are expressed in various tissues while 18 ZmPGs are highly expressed in maize anther, and their detailed expression profiles in different anther developmental stages were further investigated by using RT-qPCR analysis. These results provide valuable information for further functional characterization and application of the ZmPGs in maize.

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“…Previous studies have demonstrated that the transcription factors MS188 and MS1 participate in a genetic pathway that is important for tapetal development and pollen formation in rice and Arabidopsis (Shi et al, 2015). MS188 directly regulates the expression of RES2/QRT3, which restores the fertility of the typical P/TGMS line rvms‐2 (Shi et al, 2021). Similarly, other Arabidopsis P/TGMS mutants, such as acos5‐2, cyp703a2, npu‐2, rpg1 and cals5‐2 , which contain mutations in genes required for pollen wall development, exhibit fertility restoration under low temperature or short‐day photoperiod conditions (Zhang et al, 2020; Zhu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

MALE STERILITY 3 encodes a plant homeodomain‐finger protein for male fertility in soybean

Fan

et al. 2022

JIPB

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Male-sterile plants are used in hybrid breeding to improve yield in soybean (Glycine max (L.) Merr.). Developing the capability to alter fertility under different environmental conditions could broaden germplasm resources and simplify hybrid production. However, molecular mechanisms potentially underlying such a system in soybean were unclear. Here, using positional cloning, we identified a gene, MALE STERILITY 3 (MS3), which encodes a nuclear-localized protein containing a plant homeodomain (PHD)-finger domain. A spontaneous mutation in ms3 causing premature termination of MS3 translation and partial loss of the PHD-finger. Transgenetic analysis indicated that MS3 knockout resulted in nonfunctional pollen and no selfpollinated pods, and RNA-seq analysis revealed that MS3 affects the expression of genes associated with carbohydrate metabolism. Strikingly, the fertility of mutant ms3 can restore under longd conditions. The mutant could thus be used to create a new, more stable photoperiod-sensitive genic male sterility line for two-line hybrid seed production, with significant impact on hybrid breeding and production.

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A cellular mechanism underlying the restoration of thermo/photoperiod-sensitive genic male sterility

Cited by 29 publications

References 47 publications

Degradation of de‐esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton

Degradation of de‐esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton

Genome-Wide Identification and Characterization of Polygalacturonase Gene Family in Maize (Zea mays L.)

MALE STERILITY 3 encodes a plant homeodomain‐finger protein for male fertility in soybean

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