A cell wall preparation of <i>Enterococcus faecalis</i> strain EC‐12 stimulates β‐defensin expression in newly hatched broiler chicks

Sakai, Yuri; Tsukahara, Takamitsu; Matsubara, Noritaka; Ushida, Kazunari

doi:10.1111/j.1740-0929.2006.00410.x

Cited by 8 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were more resistant to the pathogenic effects of coliform. The antimicrobial effects of probiotics come from the volatile fatty acid and other organic acids such as lactate and succinate (Kubena et al, 2001) and through the production of bacteriocins and phage-displayed peptides (Sakai et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Influence of dietary supplementation of probiotics on intestinal histo-morphometry, blood chemistry and gut health status of broiler chickens

Biswas¹,

Junaid²,

Kumawat³

et al. 2019

SA J. An. Sci.

View full text Add to dashboard Cite

The objective of this experiment was to investigate the effects of dietary supplementation of probiotics, Lactobacillus acidophilus and Bifidobacterium bifidum, on histo-morphologic measurements of the small intestine and on the gut health status of broiler chickens. A total of 240 day-old broiler chicks were randomly assigned to one of six dietary treatments for six weeks. The dietary treatments were basal diet (T 1); basal diet + bacitracin methyl di-salicylate (BMD) at 20 mg/kg diet (T 2); basal diet + L. acidophilus (10 6 and 10 7 cfu/gm diet) (T 3 and T 4); and basal diet + B. bifidum (10 6 and 10 7 cfu/gm diet) (T 5 and T 6), respectively. The villus height (VH), width (VW), crypt depth (CD) and the ratio between villus height and crypt depth (VH: CD) were significantly increased at 21 days and 42 days in L. acidophilus-supplemented groups (T 3 or T 4). No significant differences were observed in protein, albumin and serum mineral (Ca and P) concentrations and alkaline phosphatase (ALP) activity in any of the dietary probiotic treatment groups. Aspartate aminotransferase (AST) and creatinine concentrations were increased, whereas alanine aminotransferase (ALT), uric acid and total cholesterol levels were significantly decreased in probiotic-supplemented groups. Coliforms and total anaerobes counts were significantly reduced at 21 and 42 days at the ileum and caecal in T 4 , whereas the lactic acid bacteria (LAB) Lactobacilli count was significantly increased in all the probioticsupplemented groups. The results of the present study indicate that L. acidophilus at 10 6 cfu/g feed inclusion in basal diet may improve blood biochemical parameters and histo-morphometry in the intestine and gut health in broiler chickens, resulting in a valid feed additive to replace antibiotic growth promoters.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of dietary supplementation of probiotics on intestinal histo-morphometry, blood chemistry and gut health status of broiler chickens

Biswas¹,

Junaid²,

Kumawat³

et al. 2019

SA J. An. Sci.

View full text Add to dashboard Cite

show abstract

“…Dead probiotics, on the other hand, are proven as good a biological immune modifier as viable probiotics (Sakai et al . , ; Chuang et al . ) and, moreover, are safer with longer shelf‐life (Adams ).…”

Section: Introductionmentioning

confidence: 99%

“…Dead probiotics, on the other hand, are proven as good a biological immune modifier as viable probiotics (Sakai et al 2006(Sakai et al , 2007Chuang et al 2007) and, moreover, are safer with longer shelf-life (Adams 2010). Due to the existence of pre-gastric fermentative rumen, dietary supplementation of probiotics to cattle is relatively less common.…”

Section: Introductionmentioning

confidence: 99%

Intramammary infusion of an Enterococcus faecium SF68 preparation promoted the involution of drying off Holstein cows partly related to neutrophil‐associated matrix metalloproteinase 9

Tiantong

Peng

Chen

et al. 2014

Animal Science Journal

View full text Add to dashboard Cite

A problem for dairy cows following milk stasis is to cope with a high risk of intramammary infection and there is a need to initiate an extensive renewal of secretory modules in mammary glands so that milk production in next lactation may be optimized. We recently reported that ultrasonicated Enterococcus faecium SF68 (SF68) is compatible with cow mammary glands and an enhancer of innate immunity during the immediate post-milk stasis period. The current study further examines the concomitant effect of ultrasonicated SF68 on mammary tissue remodeling. Four Holstein cows each received intramammary infusions of regular antibiotic dry-cow formula (positive control) and two different doses of SF68 in different quarters. Analyses of individual quarter secretion samples showed faster neutrophil infiltration, earlier modifications in protein composition, including caseins and lactoferrins, as well as more prompt elevation of the specific unit of 92-kDa matrix metalloproteinase 9 (MMP9) in SF68-infused quarters compared to the positive controls. Intramammary infusion of ultrasonicated SF68 seems able to accelerate the regression of mammary synthetic capacity and potentiate the breakdown of glandular extracellular matrix, indicating a more efficient mammary gland involution. Correlation analyses imply that the ability of ultrasonicated SF68 to induce faster neutrophil chemotaxis and the associated MMP9 release is partly responsible.

show abstract

“…We previously demonstrated that a cell preparation of Enterococcus faecalis strain EC‐12 (EC‐12) stimulated the immune functions in rodents (Kuramoto et al . 2004), chicks (Sakai et al . 2006, 2007) and pigs (T. Tsushima et al .…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, most of the immune stimulation by LAB is supposed to be derived by their cell wall preparations (Meydani & Ha 2000), and thus dead LAB cells may also have an immunostimulatory effect in juvenile calves. We previously demonstrated that a cell preparation of Enterococcus faecalis strain EC-12 (EC-12) stimulated the immune functions in rodents (Kuramoto et al 2004), chicks (Sakai et al 2006(Sakai et al , 2007 and pigs (T. Tsushima et al unpubl. data, 2007).…”

Section: Introductionmentioning

confidence: 99%

A cell preparation of Enterococcus faecalis strain EC‐12 stimulates the luminal immunoglobulin A secretion in juvenile calves

Tsuruta

Inoüe

Tsukahara

et al. 2009

Animal Science Journal

Self Cite

View full text Add to dashboard Cite

The immune system in juvenile calves is immature, so calves are susceptible to several diarrheal and respiratory diseases. Oral administration of lactic acid bacteria (LAB) is known to improve the growth performance and prevent diarrheal and respiratory diseases by stimulating the immune system in juvenile calves. Most of the immunostimulation by LAB is achieved by their cell wall components, and therefore we evaluated the immunostimulation of the cell preparation of Enterococcus faecalis strain EC-12 (EC-12) in juvenile calves in a clinical field. Twenty-nine 1-week old calves were used. Fourteen calves were administered 0.2% (w/w) of an EC-12 preparation that supplemented a milk replacer, and other calves were not supplemented. Feces and serum was collected at day 0, 7 and 49 after the administration to measure the IgA and IgG concentration. The fecal IgA concentration was increased by EC-12 administration at day 49, and the serum IgA concentration was also increased at day 7. These results suggested that oral administration of EC-12 in juvenile calves might have an immunostimulatory effect and provide earlier recovery of IgA levels in mucosal immunity.

show abstract

A cell wall preparation of Enterococcus faecalis strain EC‐12 stimulates β‐defensin expression in newly hatched broiler chicks

Cited by 8 publications

References 24 publications

Influence of dietary supplementation of probiotics on intestinal histo-morphometry, blood chemistry and gut health status of broiler chickens

Influence of dietary supplementation of probiotics on intestinal histo-morphometry, blood chemistry and gut health status of broiler chickens

Intramammary infusion of an Enterococcus faecium SF68 preparation promoted the involution of drying off Holstein cows partly related to neutrophil‐associated matrix metalloproteinase 9

A cell preparation of Enterococcus faecalis strain EC‐12 stimulates the luminal immunoglobulin A secretion in juvenile calves

Contact Info

Product

Resources

About