A cell line model for the differentiation of human dendritic cells

Berges, Carsten; Naujokat, Cord; Tinapp, Sarah; Wieczorek, Hubert; Höh, Alexandra; Sadeghi, Mahmoud; Opelz, Gerhard; Daniel, Volker

doi:10.1016/j.bbrc.2005.05.171

Cited by 156 publications

(154 citation statements)

References 47 publications

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“…The THP1 cell line conditionally expressing GFP was generated similarly as a control. To induce differentiation of THP1 cells toward DCs, cells were treated with the cytokines mixture as described previously (22) with some modifications. Briefly, cell were grown in 12-well plate in RPMI1640 medium supplemented with 10% FBS, 100 ng/ml GM-CSF, 50 ng/ml IL4, and TNF-␣, 100 ng/ml ionomycin for 2 days.…”

Section: Methodsmentioning

confidence: 99%

“…VentX Promotes Dendritic Cell Differentiation-Human monocytic cell line THP1, under appropriate differentiation conditions, can be induced to differentiate into immature and mature DCs with phenotypic and functional properties similar to those of primary DCs (22,24). To further explore the effects of VentX on dendritic cell differentiation, we generated stable THP1 cell lines expressing GFP or GFP.VentX under the control of doxycycline-inducible promoter.…”

Section: Cd1amentioning

confidence: 99%

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Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Gao

Bleday

et al. 2014

Journal of Biological Chemistry

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Background:The transcriptional regulation of human dendritic cells (DCs) differentiation and maturation remains incompletely understood. Results: VentX knockdown impaired DCs differentiation and maturation whereas VentX overexpression counteracted the inhibitory effects of corticosteroid on DC activation. Conclusion: VentX is critical for DCs differentiation and maturation. Significance: VentX may be a novel target to modulate DCs functions and manage inflammatory diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Cd1amentioning

confidence: 99%

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Gao

Bleday

et al. 2014

Journal of Biological Chemistry

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“…In addition to MAPKs, NFκB was also shown to play a critical role in LPS-induced CD40 expression since inhibition of NFκB activation with Bay 11-7082 significantly blocked CD40 expression on THP-1 cells exposed to LPS. Distinct roles for the five NFκB molecules including p50, p52, RelA (p65), c-Rel, and RelB have been identified in dendritic cell function (Berges et al, 2005). In this study we observed that p65 was mainly affected by LPS exposure in THP-1 cells using the supershift assay.…”

Section: Discussionmentioning

confidence: 63%

Involvement of mitogen-activated protein kinases and NFκB in LPS-induced CD40 expression on human monocytic cells

Alexis

Chen

et al. 2008

Toxicology and Applied Pharmacology

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CD40 is a costimulatory molecule linking innate and adaptive immune responses to bacterial stimuli, as well as a critical regulator of functions of other costimulatory molecules. The mechanisms regulating lipopolysaccharide (LPS)-induced CD40 expression have not been adequately characterized in human monocytic cells. In this study we used a human monocytic cell line, THP-1, to investigate the possible mechanisms of CD40 expression following LPS exposure. Exposure to LPS resulted in a dose-and time-dependent increase in CD40 expression. Further studies using immunoblotting and pharmacological inhibitors revealed that mitogen-activated protein kinases (MAPKs) and NFκB were activated by LPS exposure and involved in LPS-induced CD40 expression. Activation of MAPKs was not responsible for LPS-induced NFκB activation. TLR4 was expressed on THP-1 cells and pretreatment of cells with a Toll-like receptor 4 (TLR4) neutralizing antibody (HTA125) significantly blunted LPS-induced MAPK and NFκB activation and ensuing CD40 expression. Additional studies with murine macrophages expressing wild type and mutated TLR4 showed that TLR4 was implicated in LPS-induced ERK and NFκB activation, and CD40 expression. Moreover, blockage of MAPK and NFκB activation inhibited LPS-induced TLR4 expression. In summary, LPS-induced CD40 expression in monocytic cells involves MAPKs and NFκB.

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“…Most DC cell lines were differentiated from leukemia-derived cells, originating from myelogenous or monocytic lineage: the cytokine independent cell lines THP-1, U937, KG-1, HL-60 and the cytokine dependent cell line Mutz-3 are the most commonly used (Table 17.1 ) (Berges et al 2005 ;van Helden et al 2008 ;Santegoets et al 2008 ). The use of these lines has the advantage of the availability of large numbers of synchronized cells with a long life span, but the association with selective DC subsets in peripheral blood and mucosal surfaces is largely unclear.…”

Section: Cell Linesmentioning

confidence: 99%

Dendritic Cells

Plantinga

Haar

Nierkens

2015

The Impact of Food Bioactives on Health

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Dendritic cells (DCs) are the sentinels of the immune system and play a critical role in stimulating immune responses against pathogens and maintaining immune homeostasis to harmless antigens. They can be found in all lymphoid and most non-lymphoid tissues, including mucosal surfaces, like the lung and the gut, where intricate networks of DCs are situated to sense potential harmful exposures. As such, DCs are among the fi rst cells to come into contact with food bioactives in the gastrointestinal tract and thus are instrumental in shaping the immune system's response to such exposures. Here we provide an overview of DC characteristics, with the emphasis on DCs in the mucosal immune system, and discuss in vitro/ex vivo DC culture settings that can be applied for in vitro testing of (food) compounds.

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A cell line model for the differentiation of human dendritic cells

Cited by 156 publications

References 47 publications

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Involvement of mitogen-activated protein kinases and NFκB in LPS-induced CD40 expression on human monocytic cells

Dendritic Cells

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