A Cell-Free In Vitro Import System for Peroxisomal Proteins Containing a Type 2 Targeting Signal (PTS2)

The mammalian peroxisomal membrane is permeable to both GSH and GSSG – Implications for intraperoxisomal redox homeostasis

“…Peroxisomal in vitro import of PTS2-containing proteins employing the PNS-based system is experimentally well characterized and can be assessed by several criteria [ [47] , [48] , [49] , [50] ]. For instance, after incubation with the PNS, a fraction of the PTS2 protein should appear in the organelle pellet and should display a lower molecular mass, reflecting processing of the PTS2 peptide by the peroxisomal matrix TYSD1 protease [ 51 ].…”