A cell-free extract of Salmonella typhimurium inhibits mitogen-induced proliferation of murine splenic T lymphocytes

Matsui, K

doi:10.1016/0928-8244(94)90039-6

Cited by 2 publications

(3 citation statements)

References 14 publications

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“…Our results showed that the LT2 extract did not cleave rIL-2 ( Fig. 3), unlike Legionella, which does possess such a protease [23], and they agree with those of our previous study, which demonstrated that the suppression of T-cell proliferation was not attributable to proteases in the LT2 extract [9].…”

Section: Discussionsupporting

confidence: 93%

“…Previously, we demonstrated that the immunosuppression of T-cells in Salmonella-infected mice involved inhibition of both interleukin-2 (IL-2) secretion and IL-2 responsiveness [8]. Furthermore, the suppressive effect on IL-2mediated T-cell activation was observed in in vitro experiments using a cell-free extract (soluble fraction) of Salmonella [9]. However, the detailed mechanism(s) responsible for the immunosuppression is/are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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A cell-free Salmonella typhimurium extract modulates interleukin-2 (IL-2) receptor expression but not IL-2-stimulated responses of murine splenic lymphocytes

Matsui

1994

FEMS Immunology and Medical Microbiology

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In a previous study, we observed that a cell-free Salmonella typhimurium extract induced suppression of mitogen-induced T-cell proliferation and this suppression involved non-responsiveness of T-cells to interleukin-2 (IL-2). In this study, we found that a cell-free S. typhimurium extract modulated IL-2 receptor (IL-2R) expression on phytohemagglutinin (PHA)-stimulated murine spleen cells and this was a mechanism of T-cell non-responsiveness to IL-2, but did not affect IL-2 binding to IL-2R and the consequent responses. Western blotting using anti-phosphotyrosine antibodies showed that IL-2R-mediated tyrosine phosphorylation of protein substrates in PHA-activated murine splenic T-cells, which express a high-affinity IL-2R (alpha- and beta-chains), was not affected by treatment with the S. typhimurium cell-free extract. Furthermore, PHA-activated spleen T-cells responded to recombinant IL-2 and this was not inhibited by the extract. Surprisingly, IL-2R expression was augmented by treatment with the extract, although this was independent of IL-2 production. These results suggest that the suppression of T-cell proliferation induced by the Salmonella cell-free extract was associated with augmentation of IL-2R expression, rather than down-regulation of the IL-2 response. This may be a mechanism responsible for the Salmonella extract-evoked suppression of mitogen-induced T-cell proliferation.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

A cell-free Salmonella typhimurium extract modulates interleukin-2 (IL-2) receptor expression but not IL-2-stimulated responses of murine splenic lymphocytes

Matsui

1994

FEMS Immunology and Medical Microbiology

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“…Previously, we demonstrated that the suppression of T cells in Salmonella-infected mice involved inhibition of both interleukin-2 (IL-2) secretion and IL-2 re-sponsiveness [S]. Furthermore, a suppressive effect on IL-2-mediated T cell activation was observed in in vitro experiments using a cell-free extract (soluble fraction) of Salmonella [9] and this was associated with IL-2 receptor (a chain) expression [lo]. However, this phenomenon could not be replicated in Salmonella-infected mice.…”

Section: Introductionmentioning

confidence: 99%

Immunosuppression induced by Salmonella infection is correlated with augmentation of interleukin-2 receptor α chain expression in murine splenic lymphocytes

Matsui¹

1995

FEMS Immunology and Medical Microbiology

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In a previous study, we observed that the suppression of T-cell proliferation induced by Salmonella cell-free extract was associated with augmentation of IL-2 receptor (IL-2R) alpha chain expression. In this study, we also observed this kind of augmentation of IL-2R alpha in Salmonella-infected mice. Phytohaemagglutinin (PHA)-stimulated proliferation of murine spleen cells was significantly suppressed when the mice were infected with Salmonella typhimurium. However, expression of the alpha chain but not the beta chain of IL-2R in lymphocytes was augmented by the infection. Analysis of the IL-2R-positive cell-population showed that the augmentation of IL-2R alpha was not specific to certain cell subpopulations. Furthermore, the inhibition of PHA-stimulated murine spleen cell proliferation and the augmentation of IL-2R alpha expression induced by the infection in lymphocytes was completely reversed by treatment with anti-interferon-gamma monoclonal antibody (anti-IFN-gamma Ab). These results suggest that the suppression of T-cell proliferation induced by Salmonella infection was associated with augmentation of IL-2R alpha expression in an IFN-gamma production-dependent manner in the same way as the suppression of T-cell proliferation induced by Salmonella cell-free extract.

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A cell-free extract of Salmonella typhimurium inhibits mitogen-induced proliferation of murine splenic T lymphocytes

Cited by 2 publications

References 14 publications

A cell-free Salmonella typhimurium extract modulates interleukin-2 (IL-2) receptor expression but not IL-2-stimulated responses of murine splenic lymphocytes

A cell-free Salmonella typhimurium extract modulates interleukin-2 (IL-2) receptor expression but not IL-2-stimulated responses of murine splenic lymphocytes

Immunosuppression induced by Salmonella infection is correlated with augmentation of interleukin-2 receptor α chain expression in murine splenic lymphocytes

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