A cationic cholesterol based nanocarrier for the delivery of p53-EGFP-C3 plasmid to cancer cells

Misra, Santosh K.; Naz, Sarwat; Kondaiah, Paturu; Bhattacharya, Santanu

doi:10.1016/j.biomaterials.2013.10.062

Cited by 70 publications

(64 citation statements)

References 57 publications

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“…Other groups have reported the electrostatic interaction when the GCL contains an oligo-oxyethylene spacer [93,115], and/or other headgroups as imidazolium or pyridinium [45, 57-59, 75, 99, 116]. Looking for more biocompatible vectors, GCLs that incorporate aminoacid rests, such as GCL-lysine 12 based and GCL-arginine based [60,62,64], GCL-cholesterol based [117][118][119], and GCL-cyclen-based [120] have been also investigated. Lipoplexes containing a MVCL with dendritic-type headgroup have been also analyzed [67,121,122].…”

Section: Electrostatic Interactionmentioning

confidence: 97%

“…As a result of that, the transfection capability [75]. Recent studies reporting TE of lipoplexes and polyplexes have been investigated: (i) divalent GCLs, as cholesterol-based with alkyl or oligooxyethylene spacers [118,119,121], aromatic-based with alkyl or oligo-oxyethylene spacers [46,93], SS14 linkers [66], ammonium with alkyl or oligo-oxyethylene spacers [54,71,93,179], imidazolium or pyridinium derivatives with alkyl-or oligooxyethylene spacers [45,59,75], aminoacid-based [62,64] or gemini-like amphiphilic peptides [150], cyclen-based CLs [120,180], and archael tetraether-based lipids [138];…”

Section: Biochemistry Characterizationmentioning

confidence: 99%

“…The results confirm that all these optimized formulations did not report any toxicity to the cell lines studied, the cell viability after transfection being considerably better than the commercial control Lipo2000. The cell viability/cytotoxicity of several complexes between nucleic acids and a wide variety of recent synthesized MVCVs has been analyzed: (i) GCLs as cholesterol-based with alkyl or oligo-oxyethylene spacers [118,119,121,206,207], aromatic-based with alkyl or oligo-oxyethylene spacers [46], ammonium derivatives [54,71,93] imidazolium derivatives [45,75,99] and pyridinium derivatives [57] with alkyl type spacers, aminoacid-based [64,208], SS14 linkers [66], gemini-like amphiphilic peptides [150] or cyclen-based CLs [120,180]; (ii) several MVCLs [67,72,122,181,182]; (iii) cationic polymer-derivatives from PEI [124,127,183,209], PAMAM [140], PDMAEMA [123,133], chitosan [188], polyphosphoramidates [189], polyhydroxyalkanoates [92], polyamines [107,187] or polyaminoacids [127,190]; (iv)…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

Junquera

Aicart

2016

Advances in Colloid and Interface Science

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Section: Electrostatic Interactionmentioning

confidence: 97%

Section: Biochemistry Characterizationmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

See 1 more Smart Citation

Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

Junquera

Aicart

2016

Advances in Colloid and Interface Science

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“…Mononuclear cells were isolated from wound tissue homogenates, bone marrow, spleen or kidney, as described in the literature [18,19]. For quantification of GFP + MSC engraftment in wounded skin, four to six wounds and 4 mm of surrounding skin were excised at day 7 post-wounding.…”

Section: Mononuclear Cell Isolation and Flow Cytometric Analysismentioning

confidence: 99%

JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells

Xiao

et al. 2015

Clinical Science

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The homing ability and secretory function of mesenchymal stem cells (MSCs) are key factors that influence cell involvement in wound repair. These factors are controlled by multilayer regulatory circuitry, including adhesion molecules, core transcription factors (TFs) and certain other regulators. However, the role of adhesion molecules in this regulatory circuitry and their underlying mechanism remain undefined. In the present paper, we demonstrate that an adhesion molecule, junction adhesion molecule A (JAM-A), may function as a key promoter molecule to regulate skin wound healing by MSCs. In in vivo experiments, we show that JAM-A up-regulation promoted both MSC homing to full-thickness skin wounds and wound healing-related cytokine secretion by MSCs. In vitro experiments also showed that JAM-A promoted MSC proliferation and migration by activating T-cell lymphoma invasion and metastasis 1 (Tiam1). We suggest that JAM-A up-regulation can increase the proliferation, cytokine secretion and wound-homing ability of MSCs, thus accelerating the repair rate of full-thickness skin defects. These results may provide insights into a novel and potentially effective approach to improve the efficacy of MSC treatment.

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“…for: therapeutic angiogenesis, regeneration of peripheral nerves and bones (see review [4]), cancer treatment [5], and DNA vaccination [6]. However, the improvement of intrinsically low pDNA transfection efficiency remains relevant and unresolved biomedical task.…”

Section: Introductionmentioning

confidence: 99%

Intracellular delivery of VEGF165 encoding gene therapeutic using trifunctional copolymers of ethylene oxide and propylene oxide

Bondar

Shevchenko

Мартынова

et al. 2015

European Polymer Journal

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New type of copolymers of propylene oxide and ethylene oxide was assessed for promoting delivery of plasmid DNA based gene therapeutics. Lipid-like trifunctional copolymers (TFCs), with both random or diblock structures and relatively low hydrophilic-lipophilic balance, were studied and compared with linear Pluronic™ L61. Structure-dependent relationships for micelleforming, cytotoxic and hemolytic properties of these copolymers were revealed. The TFC with the mean number of propylene oxide and ethylene oxide units of 83.5 and 24.2, respectively, exhibited relatively low adverse effects in vitro. The latter TFC interacted with plasmid DNApolyethyleneimine complexes and improved their intracellular delivery. Furthermore, this TFC efficiently promoted the transfection of dermal fibroblasts with VEGF165-encoding Neovasculgen® plasmid DNA, which has been clinically used for the therapeutic angiogenesis.Our findings demonstrated for the first time that TFCs are promising for the polymer-mediated delivery of gene therapeutics.

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A cationic cholesterol based nanocarrier for the delivery of p53-EGFP-C3 plasmid to cancer cells

Cited by 70 publications

References 57 publications

Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells

Intracellular delivery of VEGF165 encoding gene therapeutic using trifunctional copolymers of ethylene oxide and propylene oxide

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