A Cation-regulated and Proton Gradient-dependent Cation Transporter from Chlamydomonas reinhardtii Has a Role in Calcium and Sodium Homeostasis

Pittman, Jon K.; Edmond, Clare; Sunderland, Paul A.; Bray, Clifford M.

doi:10.1074/jbc.m807173200

Cited by 49 publications

(58 citation statements)

References 49 publications

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“…The first direct evidence of Na + transport mediated by a CAX from a photosynthetic organism came from work in the green alga Chlamydomonas reinhardtii (Pittman et al . 2009), yet Na + transport activity for a higher plant CAX has not been seen, apart from vacuolar Na + sequestration via Na + /Ca 2+ exchange by AtNCL (Li et al . 2016).…”

Section: The Cax Transporters In Abiotic Stress Signallingmentioning

confidence: 99%

“…Future work may also utilise CAX from microalgae such as Chlamydomonas to remediate Cd 2+ ‐polluted freshwater sites (Pittman et al . 2009). …”

Section: The Cax Transporters In Biotechnologymentioning

confidence: 99%

“…Parasite CAXs, such as from the malarial parasites Plasmodium , share phylogenetic and functional similarities with higher plant and algal CAXs (Pittman et al . 2009; Guttery et al . 2013), yet the subcellular CAX localisation appears to differ, despite apicomplexan parasites possessing plant‐like vacuoles.…”

Section: Non‐plant Caxs – Lessons To Be Learned?mentioning

confidence: 99%

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CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

2016

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Cation/proton exchangers (CAXs) are a class of secondary energised ion transporter that are being implicated in an increasing range of cellular and physiological functions. CAXs are primarily Ca2+ efflux transporters that mediate the sequestration of Ca2+ from the cytosol, usually into the vacuole. Some CAX isoforms have broad substrate specificity, providing the ability to transport trace metal ions such as Mn2+ and Cd2+, as well as Ca2+. In recent years, genomic analyses have begun to uncover the expansion of CAXs within the green lineage and their presence within non‐plant species. Although there appears to be significant conservation in tertiary structure of CAX proteins, there is diversity in function of CAXs between species and individual isoforms. For example, in halophytic plants, CAXs have been recruited to play a role in salt tolerance, while in metal hyperaccumulator plants CAXs are implicated in cadmium transport and tolerance. CAX proteins are involved in various abiotic stress response pathways, in some cases as a modulator of cytosolic Ca2+ signalling, but in some situations there is evidence of CAXs acting as a pH regulator. The metal transport and abiotic stress tolerance functions of CAXs make them attractive targets for biotechnology, whether to provide mineral nutrient biofortification or toxic metal bioremediation. The study of non‐plant CAXs may also provide insight into both conserved and novel transport mechanisms and functions.

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Section: The Cax Transporters In Abiotic Stress Signallingmentioning

confidence: 99%

“…Future work may also utilise CAX from microalgae such as Chlamydomonas to remediate Cd 2+ ‐polluted freshwater sites (Pittman et al . 2009). …”

Section: The Cax Transporters In Biotechnologymentioning

confidence: 99%

Section: Non‐plant Caxs – Lessons To Be Learned?mentioning

confidence: 99%

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CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

2016

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“…It was shown that CAX1 from the chlorophyllian unicellular alga Chlamydomonas reinhardtii mediates vacuolar proton gradient-dependent Ca 2ϩ and Na ϩ exchange activity when expressed in yeast (23). Furthermore, co-expression of AtCAX1 and AtCAX3 in yeast cells leads to the formation of "hetero-CAX" complexes able to transport Li ϩ without affecting Ca 2ϩ transport rate (24).…”

mentioning

confidence: 99%

Vacuolar Cation/H+ Antiporters of Saccharomyces cerevisiae*

Cagnac¹,

Aranda-Sicilia²,

Leterrier³

et al. 2010

Journal of Biological Chemistry

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“…Correspondingly, these CAXs improve Ca 2+ accumulation (Hirschi, 1999), enhance tolerance to Cd 2+ (Korenkov et al, 2007a, b;Wu et al, 2011), and Li + and Na + (Luo et al, 2005) when expressed constitutively in plant tissues. In addition, some of CAXs are also involved in plant various stresses responses, including low temperatures, dehydration and high salt (Knight et al, 1996;Pittman et al, 2009;Han et al, 2011). Nevertheless, the fact that Atcax1 mutant showed stronger cold acclimation response and CAX1 transgenic tobacco had higher cold sensitivity, but cax3 mutant exhibited reduced salt tolerance, these demonstrated that different CAXs have distinct characteristics and play diverse roles in plant adaptation to various environmental conditions (Hirschi, 1999;Catala et al, 2003;Zhao et al, 2008).…”

Section: +mentioning

confidence: 99%

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

Li¹,

Zhou²,

Zheng³

et al. 2017

IJAB

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The CAX (Calcium Exchanger) genes constitute a set of signal transductors that are important for plant growth and adaptation to environmental stresses, especially to low temperature. CbCAX51, a Ca 2+ /H + exchanger was cloned form Capsella bursa-pastoris, has been reported as a cold inducible gene. Here we showed that the transcriptional level of CbCAX51 in leaf and stem was higher than that in roots. Transcript of CbCAX51 was continuously up-regulated by Ca 2+ and Cu 2+, and transiently up-regulated by Zn 2+ and Li + , cold stress, GA and ABA. The CbCAX51 protein was predicted to be localized in vacuolar by PSORT to Plant-mPLos, which was further confirmed by the subcellular detection of CbCAX51-GFP fusion protein. When CbCAX51 was transformed into the cold-sensitive plant tobacco, it conferred cold tolerance in tobacco seedlings based on physiological studies. Likewise, overexpression of CbCAX51 can activated some cold responsive genes in transgenic tobacco. Taken together, CbCAX51 is involved in the cold signal transduction and modulation of downstream components to enhance cold tolerance in tobacco.

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A Cation-regulated and Proton Gradient-dependent Cation Transporter from Chlamydomonas reinhardtii Has a Role in Calcium and Sodium Homeostasis

Cited by 49 publications

References 49 publications

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

Vacuolar Cation/H+ Antiporters of Saccharomyces cerevisiae*

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

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A Cation-regulated and Proton Gradient-dependent Cation Transporter from Chlamydomonas reinhardtii Has a Role in Calcium and Sodium Homeostasis

Cited by 49 publications

References 49 publications

CAX‐ing a wide net: Cation/H+ transporters in metal remediation and abiotic stress signalling

CAX‐ing a wide net: Cation/H+ transporters in metal remediation and abiotic stress signalling

Vacuolar Cation/H+ Antiporters of Saccharomyces cerevisiae*

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

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CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling