A case report of cutaneous leishmaniasis: a misleading clinical presentation

Vanlier, Corentine; Laranaga, Eva; Dabadie, P.; Yombi, Jean‐Cyr; Yıldız, Halil; Baeck, Marie

doi:10.1007/s15010-020-01517-1

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…The traditional detection techniques ( Kromer et al., 2019 ) mostly include histopathology, skin tissue culture, and PCR-bases methods ( Abdalla et al., 2009 ). Histopathologic analyses are nonspecific usually manifesting in the form of granulomatous inflammatory cell infiltrates that are difficult to differentiate from syphilis, granulomatous rosacea, skin tumor ( Teh et al., 2013 ), cutaneous leishmaniasis ( Vanlier et al., 2021 ) and deep dermatophytosis. Thus, histopathologic diagnosis is generally combined with Ziehl-Neelsen staining, particularly for lesions that have a high bacterial load.…”

Section: Discussionmentioning

confidence: 99%

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Wang

Gao

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionThe clinical practicability of DNA microarray chip in detecting the presence of mycobacterial species/isolates directly in the skin tissues has not been evaluated, nor the efficacy of DNA microarray chip as a novel diagnostic tool for the early diagnosis of cutaneous mycobacterial infections is known.MethodsThe present study analyzed the incidence of cutaneous mycobacterial infections in Shanghai and explored the efficacy of a novel DNA microarray chip assay for the clinical diagnosis of the disease from skin tissue specimens compared to traditional detection methods. A total of 60 participants fulfilling the defined diagnostic criteria and confirmed positive for cutaneous mycobacterial infections from 2019 to 2021 were enrolled in the study. Subsequent to recording the participants’ medical history and clinical characteristics, the skin tissue specimens were collected for analyses. The specimens underwent histopathological analyses, skin tissue culture, and DNA microarray chip assay.ResultsIncreased incidence of cutaneous mycobacterial infection was detected from 2019 to 2021. The most common infecting pathogen was M. marinum followed by M. abscessus. The sensitivity, specificity and accuracy of the skin tissue culture method were 70%, 100% and 76.62%, respectively, while that of the DNA microarray chip assay were 91.67%, 100% and 93.51%, respectively. The sensitivity and accuracy of the DNA microarray chip assay were significantly higher than those of the skin tissue culture method. The positive likelihood and diagnostic odds ratio were >10 and >1, respectively for both the methods. The negative likelihood ratio was significantly higher (30% vs 8.33%) and the Youden’s index was significantly lower (70.00% vs 91.67%) in the skin culture method compared to that of the DNA microarray chip assay. There was a significant association of false negative results with a history of antibiotic use in the skin tissue culture method.DiscussionGiven the increasing incidence of cutaneous mycobacterial infections, early diagnosis remains a prime clinical focus. The DNA microarray chip assay provides a simple, rapid, high-throughput, and reliable method for the diagnosis of cutaneous mycobacterial infections with potential for clinical application.

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Section: Discussionmentioning

confidence: 99%

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Wang

Gao

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Klinisch atypische Formen scheinen bei L. infantum häufiger vorzukommen und vermehrt zu Fehldiagnosen wie ubiquitäre Mykobakteriose, kutane Sarkoidose, kutanes Lymphom oder wie in unseren Fall orofaziale Granulomatose zu führen [4]. Aufgrund der zum Teil sehr langen Inkubationszeit (in unserem Fall mehr als ein Jahr) wird die KL oft erst spät erkannt und nicht mit einem zurückliegenden Urlaub in Verbindung gebracht [7,8].…”

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“…Zur Therapie der komplizierten, durch L. infantum verursachten KL werden in den letzten Jahren hauptsächlich liposomales Amphotericin B oder Miltefosin eingesetzt. Unter beiden Substanzen wurden zufriedenstellende Abheilungen, aber auch immer wieder Therapieversagen beschrieben, welches teilweise durch Infektionen mit Amphotericin B-oder Miltefosin-resistenten L. infantum-Stämmen bedingt war [7,[10][11][12][13]. Amphotericin B wird in der Regel von jüngeren (und erwachsenen) Patienten gut vertragen, bei älteren Patienten muss jedoch das Risiko eines Nierenversagens berücksichtigt werden.…”

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