A case of severe cholera imported from Bangladesh to Italy, 2017

Ricaboni, Davide; Bozzoni, Marina; Sforza, G; Rimoldi, Sara Giordana; Antinori, Spinello

doi:10.1016/j.tmaid.2019.05.001

Cited by 5 publications

(4 citation statements)

References 4 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we do not rule out the possibilities of other four culture-positive isolates as Vibrio spp., without screening them with other primers available for the detection of Vibrio spp. Vibrio is the major cause of diarrhea in many countries of the world including Bangladesh [6,33]. A study conducted in Nepal showed that variation in geographical locations or primer used greatly influence the occurrence and detection of various Vibrio spp.…”

Section: Resultsmentioning

confidence: 99%

Molecular detection of Vibrio cholerae from human stool collected from SK Hospital, Mymensingh, and their antibiogram

et al. 2019

View full text Add to dashboard Cite

Objective:Vibrio spp., particularly, Vibrio cholerae is a major etiology of diarrhea in humans worldwide. In this study, we isolated and identified V. cholerae from the human stool of suspected cases along with antibiogram.Materials and Methods:In total, 25 stool samples from cholera suspected patients were analyzed. Isolation and molecular detection of Vibrio species were performed based on staining, motility, cultural and biochemical characteristics followed by polymerase chain reaction (PCR) using groEL gene-specific primers.Results:Among the 25 samples, seven showed growth of yellow color colonies on Thiosulfate-Citrate-Bile salts-Sucrose agar plates. The isolates were Gram-negative, curved shaped, and motile. Biochemically, they were found positive for indole and Methyl Red tests and negative for Voges–Proskauer test. Out of the seven positive samples, only three isolates were confirmed as Vibrio spp. using genus-specific primers. Subsequently, these three isolates were confirmed as V. cholerae by PCR using V. cholerae groEL gene-specific primers. Antibiotic sensitivity test revealed these three isolates as highly sensitive to azithromycin, chloramphenicol, gentamicin, and norfloxacillin while resistant to streptomycin, tetracycline, and oxacillin.Conclusion:Vibrio cholerae were isolated from the stool of diarrheic human patients and confirmed by PCR targeting the groEL gene. The isolates were found resistant to streptomycin, tetracycline and oxacillin, and need further characterization to reveal the molecular basis of their origin and resistance.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular detection of Vibrio cholerae from human stool collected from SK Hospital, Mymensingh, and their antibiogram

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Diagnosis and reporting of cholera cases in non-endemic countries is very rare. Ricaboni et al described only 14 single imported cases diagnosed in Europe and the United States of America and in the literature published in the years from 1994 to 2018, only three of these cases were observed in Italy, in 2005, 2013 and 2017, respectively [ 48 ]. It is noteworthy that the characteristics of the case reported herein are similar to previous cases reported in Italy and other high-income countries.…”

Section: Discussionmentioning

confidence: 99%

A Cholera Case Imported from Bangladesh to Italy: Clinico-Epidemiological Management and Molecular Characterization in a Non-Endemic Country

Russini,

Giancola,

Brunetti

et al. 2023

TropicalMed

View full text Add to dashboard Cite

Despite the number of cholera outbreaks reported worldwide, only a few cases are recorded among returning European travellers. We describe the case of a 41-year-old male, returning to Italy after a stay in Bangladesh, his origin country, who presented with watery diarrhoea. Vibrio cholerae and norovirus were detected in the patient’s stools via multiplex PCR methods. Direct microscopy, Gram staining, culture and antibiotic susceptibility tests were performed. The isolates were tested using end-point PCR for the detection of potentially enteropathogenic V. cholera. Serotype and cholera toxins identification were carried out. Whole genome sequencing and bioinformatics analysis were performed, and antimicrobial resistance genes identified. A phylogenetic tree with the most similar genomes of databases previously described was built. Sample of the food brought back by the patient were also collected and analysed. The patient was diagnosed with V. cholerae O1, serotype Inaba, norovirus and SARS-CoV-2 concomitant infection. The isolated V. cholerae strain was found to belong to ST69, encoding for cholera toxin, ctxB7 type and was phylogenetically related to the 2018 outbreak in Dhaka, Bangladesh. Adopting a multidisciplinary approach in a cholera non-endemic country ensured rapid and accurate diagnosis, timely clinical management, and epidemiological investigation at national and international level.

show abstract

“…Most people infected with V. cholerae do not develop any symptoms and bacteria are present in faeces for 1e10 days after infection [1]; other authors [2] have shown shedding beyond 10 days. The global burden of cholera is borne almost entirely by those countries where basic infrastructure is available for only a minority of the population [3]; in recent years only a few sporadic cases of imported V. cholerae infections in high income countries have been described [4].…”

Section: To the Editormentioning

confidence: 99%

Suspected cholera case (imported), associated with other gastrointestinal pathogens, in Bolzano, Northern Italy

Aschbacher

Mercolini

Lucarelli

et al. 2020

Clinical Microbiology and Infection

View full text Add to dashboard Cite

A case of severe cholera imported from Bangladesh to Italy, 2017

Cited by 5 publications

References 4 publications

Molecular detection of Vibrio cholerae from human stool collected from SK Hospital, Mymensingh, and their antibiogram

Molecular detection of Vibrio cholerae from human stool collected from SK Hospital, Mymensingh, and their antibiogram

A Cholera Case Imported from Bangladesh to Italy: Clinico-Epidemiological Management and Molecular Characterization in a Non-Endemic Country

Suspected cholera case (imported), associated with other gastrointestinal pathogens, in Bolzano, Northern Italy

Contact Info

Product

Resources

About