A case of paracoccidioidomycosis due to Paracoccidioides lutzii presenting sarcoid-like form

Tatagiba, Luiza Sarmento; Pivatto, Luana Bridi; Faccini-Martínez, Álvaro A.; Peçanha, Paulo Mendes; Velloso, Tânia Regina Grão; Gonçalves, Sarah Santos; Rodrigues, Anderson Messias; Camargo, Zoilo Pires de; Falqueto, Aloísio

doi:10.1016/j.mmcr.2017.09.002

Cited by 9 publications

(8 citation statements)

References 11 publications

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“…While the former presents a case, whose fungal agent identified by serologic tools was P . lutzii [47], the latter reports a case due to P . brasiliensis molecularly identified in which serology reacted only against P .…”

Section: Resultsmentioning

confidence: 99%

Clinical features and genetic background of the sympatric species Paracoccidioides brasiliensis and Paracoccidioides americana

Macedo¹,

Teixeira

Barker

et al. 2019

PLoS Negl Trop Dis

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Introduction The agents of paracoccidioidomycosis, historically identified as Paracoccidioides brasiliensis , are in fact different phylogenetic species. This study aims to evaluate associations between Paracoccidioides phylogenetic species and corresponding clinical data. Methods Paracoccidioides strains from INI/Fiocruz patients (1998–2016) were recovered. Socio-demographic, epidemiological, clinical, serological, therapeutic and prognostic data of the patients were collected to evaluate possible associations of these variables with the fungal species identified through partial sequencing of the ADP-ribosylation factor ( arf ) and the 43-kDa-glycoprotein ( gp43 ) genes. Results Fifty-four fungal strains were recovered from 47 patients, most (72.3%) infected in Rio de Janeiro state, Brazil. Forty-one cases were caused by Paracoccidioides brasiliensis and six by Paracoccidioides americana (former PS2). P . brasiliensis was responsible for severe lymph abdominal forms, whereas patients infected with P . americana presented a high rate of adrenal involvement. However, no statistically significant associations were found for all variables studied. P . americana presented 100% reactivity to immunodiffusion, even when tested against antigens from other species, while negative results were observed in 9 (20%) cases caused by P . brasiliensis , despite being tested against a homologous antigen. Conclusions P . brasiliensis and P . americana are sympatric and share similar clinical features and habitat, where they may compete for similar hosts.

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Section: Resultsmentioning

confidence: 99%

Clinical features and genetic background of the sympatric species Paracoccidioides brasiliensis and Paracoccidioides americana

Macedo¹,

Teixeira

Barker

et al. 2019

PLoS Negl Trop Dis

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“…Recently, sparse cases related to PS3 have been found outside Colombia, in Argentina, Peru, and Brazil ( Figure 2 ) [ 19 , 23 , 24 ]. P. lutzii , on the other hand, encompasses a single species and is predominantly distributed in the Midwest and Amazon regions of Brazil and Ecuador ( Figure 2 ) [ 25 , 26 ]. The natural habitat of P. lutzii remains to be elucidated, as this species has not yet been isolated from armadillos [ 27 , 28 ].…”

Section: Paracoccidioidomycosis: An Overviewmentioning

confidence: 99%

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Pinheiro

Hahn

Camargo

et al. 2020

JoF

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Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 °C and as budding yeasts when cultured at 37 °C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus.

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“…The offshoot P. lutzii encompasses a single species. The epicenter of P. lutzii mycosis is in the Midwest and Amazon regions of Brazil, with a single case reported in Ecuador [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii

Rodrigues

Kubitschek-Barreira

Pinheiro

et al. 2020

JoF

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Paracoccidioidomycosis (PCM) is a life-threatening systemic infection caused by the fungal pathogen Paracoccidioides brasiliensis and related species. Whole-genome sequencing and stage-specific proteomic analysis of Paracoccidioides offer the opportunity to profile humoral immune responses against P. lutzii and P. brasiliensis s. str. infection using innovative screening approaches. Here, an immunoproteomic approach was used to identify PCM-associated antigens that elicit immune responses by combining 2-D electrophoresis of P. lutzii and P. brasiliensis proteomes, immunological detection using a gold-standard serum, and mass spectrometry analysis. A total of 16 and 25 highly immunoreactive proteins were identified in P. lutzii and P. brasiliensis, respectively, and 29 were shown to be the novel antigens for Paracoccidioides species, including seven uncharacterized proteins. Among the panel of proteins identified, most are involved in metabolic pathways, carbon metabolism, and biosynthesis of secondary metabolites in both immunoproteomes. Remarkably, six isoforms of the surface-associated enolase in the range of 54 kDa were identified as the major antigens in human PCM due to P. lutzii. These novel immunoproteomes of Paracoccidioides will be employed to develop a sensitive and affordable point-of-care diagnostic assay and an effective vaccine to identify infected hosts and prevent infection and development of human PCM. These findings provide a unique opportunity for the refinement of diagnostic tools of this important neglected systemic mycosis, which is usually associated with poverty.

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A case of paracoccidioidomycosis due to Paracoccidioides lutzii presenting sarcoid-like form

Cited by 9 publications

References 11 publications

Clinical features and genetic background of the sympatric species Paracoccidioides brasiliensis and Paracoccidioides americana

Clinical features and genetic background of the sympatric species Paracoccidioides brasiliensis and Paracoccidioides americana

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii

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