A Case of Ochrobactrum intermedium Bacteremia Secondary to Cholangitis With a Literature Review

Kassab, Ihab; Sarsam, Nadine; Affas, Saif; Ayas, Mohamad Fekredeen; Baang, Ji Hoon

doi:10.7759/cureus.14648

Cited by 7 publications

(11 citation statements)

References 20 publications

(58 reference statements)

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“…In order to accurately identify O. anthropi and O. intermedium , molecular typing was recommended, however, it was more time-consuming and complicated compared with MALDI-TOF. In this case, the pathogen was initially identified as O. intermedium by MALDI-TOF, which was consistent with previous study [ 8 , 13 , 17 ]. ANI result confirmed the precise identification of O. intermedium at genomic level, indicating MALDI-TOF is a promising tool for rapid identification of O. intermedium in clinical situations, though more studies are required.…”

Section: Discussionsupporting

confidence: 91%

“…In two cases, O. intermedium was isolated from fecal in patients suffering from either bacteremia or bacteremia associated with liver abscess, indicating that the gastrointestinal tract was possibly the source of infection [ 7 , 12 ]. Of the nine cases, seven patients male (so do our case), five patients had bacteremia, two of whom were associated with catheters [ 2 , 13 ]. O. anthropi infection is usually seen in immunocompromised patients with indwelling catheters, and catheter-related bacteremia is also most reported in immunocompromised children, especially among boys under 10 years old with malignancies [ 6 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

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The first case of Ochrobactrum intermedium bacteremia in a pediatric patient with malignant tumor

Jiang

Zhou

et al. 2021

BMC Infect Dis

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Background Ochrobactrum spp. are non-fermenting, Gram-negative bacilli that are regarded as emerging human pathogens of low virulence that can cause infections. The first identified case of Ochrobactrum intermedium was reported in 1998 in a liver transplantation patient with liver abcess. There are no reports of infections in pediatric patients. Here, we report the first case of O. intermedium bacteremia in a pediatric patient. Case presentation A two and a half years old male was admitted with fever, chills and nausea. He had been diagnosed as pineoblastoma and underwent surgical resection and chemotherapy. O. intermedium was isolated from his blood cultures and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), however, the Vitek II automated system failed to identify the organism. Then the pathogen was confirmed by 16S rDNA sequencing and average nucleotide identity result (ANI) confirmed the precise identification of O. intermedium at genomic level. In addition, the patient recovered well after antibiotic combined therapy. Conclusions This, to our knowledge, is the first case of O. intermedium bacteremia in a pediatric patient with malignant tumor. Traditional biochemical identification methods such as API 20NE or VITEK2 system cannot differentiate O. anthropi and O. intermedium. MALDI-TOF may be a promising tool for rapid identification of microorganisms such as O. intermedium.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The first case of Ochrobactrum intermedium bacteremia in a pediatric patient with malignant tumor

Jiang

Zhou

et al. 2021

BMC Infect Dis

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“…On the other hand, Brucella species cause the common zoonotic infection brucellosis (Godfroid et al, 2011). B. intermedia is reported as a rare opportunistic pathogen (Möller et al, 1999;Apisarnthanarak et al, 2005;Bharucha et al, 2017;Kassab et al, 2021). Clinical isolates usually express high antibiotic resistance levels (Teyssier et al, 2005;Thoma et al, 2009).…”

Section: Opportunistic Human Pathogensmentioning

confidence: 99%

Thermophilic Composting of Human Feces: Development of Bacterial Community Composition and Antimicrobial Resistance Gene Pool

et al. 2022

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In times of climate change, practicing sustainable, climate-resilient, and productive agriculture is of primordial importance. Compost from different resources, now treated as wastes, could be one form of sustainable fertilizer creating a resilience of agriculture to the adverse effects of climate change. However, the safety of the produced compost regarding human pathogens, pharmaceuticals, and related resistance genes must be considered. We have assessed the effect of thermophilic composting of dry toilet contents, green cuttings, and straw, with and without biochar, on fecal indicators, the bacterial community, and antibiotic resistance genes (ARGs). Mature compost samples were analyzed regarding fecal indicator organisms, revealing low levels of Escherichia coli that are in line with German regulations for fertilizers. However, one finding of Salmonella spp. exceeded the threshold value. Cultivation of bacteria from the mature compost resulted in 200 isolates with 36.5% of biosafety level 2 (BSL-2) species. The majority is known as opportunistic pathogens that likewise occur in different environments. A quarter of the isolated BSL-2 strains exhibited multiresistance to different classes of antibiotics. Molecular analysis of total DNA before and after composting revealed changes in bacterial community composition and ARGs. 16S rRNA gene amplicon sequencing showed a decline of the two most abundant phyla Proteobacteria (start: 36–48%, end: 27–30%) and Firmicutes (start: 13–33%, end: 12–16%), whereas the abundance of Chloroflexi, Gemmatimonadetes, and Planctomycetes rose. Groups containing many human pathogens decreased during composting, like Pseudomonadales, Bacilli with Bacillus spp., or Staphylococcaceae and Enterococcaceae. Gene-specific PCR showed a decline in the number of detectable ARGs from 15 before to 8 after composting. The results reveal the importance of sufficiently high temperatures lasting for a sufficiently long period during the thermophilic phase of composting for reducing Salmonella to levels matching the criteria for fertilizers. However, most severe human pathogens that were targeted by isolation conditions were not detected. Cultivation-independent analyses also indicated a decline in bacterial orders comprising many pathogenic bacteria, as well as a decrease in ARGs. In summary, thermophilic composting could be a promising approach for producing hygienically safe organic fertilizer from ecological sanitation.

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“…Members of the genus Brucella are Gram-negative aerobic bacilli ( 1 ), belonging to the Brucellaceae family ( 2 , 3 ), and are known as human and animal pathogens ( 2 ). Based on recent and thorough genome comparisons, the genus Ochrobactrum has been reclassified and its species included within the genus Brucella ( 4 , 5 ).…”

Section: Announcementmentioning

confidence: 99%

The Genome Sequence of Brucella intermedia DF13, a 2,4-Dichlorophenoxyacetic Acid-Degrading Soil Bacterium Isolated in Brazil

Silva

Peckle

Ribeiro

et al. 2022

Microbiol Resour Announc

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A Case of Ochrobactrum intermedium Bacteremia Secondary to Cholangitis With a Literature Review

Cited by 7 publications

References 20 publications

The first case of Ochrobactrum intermedium bacteremia in a pediatric patient with malignant tumor

The first case of Ochrobactrum intermedium bacteremia in a pediatric patient with malignant tumor

Thermophilic Composting of Human Feces: Development of Bacterial Community Composition and Antimicrobial Resistance Gene Pool

The Genome Sequence of Brucella intermedia DF13, a 2,4-Dichlorophenoxyacetic Acid-Degrading Soil Bacterium Isolated in Brazil

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