A case of gastric adenocarcinoma with conspicuous binuclear cytologic features

Ito, Hiroki; Furuya, Chiemi; Yamanouchi, Tsuyoshi; Ikemoto, Keiko; Katoh, Takahiko; Onoda, Masahiko; Kondo, Taiji; Oga, Atsunori

doi:10.1016/j.cancergen.2013.08.001

Cited by 1 publication

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“…Binuclear or polynuclear cells have also been suggested as precursors of CDCs; however, we were unable to distinguish these cells using the current methods. In a previous study, we analyzed binuclear cancer cells in ascites , and we are currently developing a method for analyzing the relationship between binuclear cells and CDCs in vitro.…”

Section: Discussionmentioning

confidence: 99%

Analysis of centromere signal patterns in breast cancer cells with chromosomal instability using image cytometry combined with centromere fluorescence in situ hybridization

et al. 2014

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Fluorescence in situ hybridization (FISH) with centromeric probes is a method used to detect chromosomal instability (CIN), a hallmark of most cancers. However, no studies thus far have investigated the relationship between centromeric FISH signals and the cell cycle in cancer cells. In this study, the chromosome content in each cell cycle phase was evaluated with respect to the number of centromeric FISH signals in two breast cancer cell lines and eight surgically resected breast cancer specimens using image cytometry. Variations in chromosome number were detected at each phase of the cell cycle but were not associated with proliferative capacity in the cell lines. Furthermore, the chromosome doubling frequency differed in each cell line and clinical specimen. These results reveal two aspects of centromeric FISH signal variation in breast cancers that exhibit CIN, and suggest that chromosome doubling is a remarkable occurrence that may increase the heterogeneity of tumors. V C 2014 International Society for Advancement of Cytometry

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Section: Discussionmentioning

confidence: 99%