A Case of Diffuse Peritoneal Larval Mesocestoides corti (syn. M. vogae) Cestodiasis in A Dog in Turkey

Abstract

SummaryA laparotomy was performed in an eleven years-old female Doberman with loss of appetite, abdominal distension and signs of pain revealed multiple structures of a size reaching 1.5 cm, composed of small (6 mm or less), white, cystoid structures, with a total volume of approximately 2 liters. To follow the development of the parasitic organisms, under microscopic observation, their body fragments were also moving without changing place which came out of the abdominal cavity, these masses were transferred to tissue culture flasks containing RPMI containing 20% fetal calf serum and placed in a humidified incubator with 5% CO 2 at 37 o C. The dog survived only one week after the surgery and the autopsy showed macroscopic parasitic material, either free-floating in the peritoneal cavity or adhering to the peritoneum and viscera. A perforation one cm in diameter was observed in pyloric area on the major curvature, covered by the omentum. No parasites were present in the intestines. Microscopically there were multiple pyrogranulomatous peritonitis, hepatitis and splenitis. The morphologic features of the parasitic material collected at laparotomy were compatible with that of Mesocestoides spp. larvae. A PCR amplification was performed with mitochondrial 12S rDNA cestod-specific primers. The sequence of the isolates was 89-100% compatible with the reference Mesocestoides corti isolates published in the National Center for Biotechnology Information (NCBI) GenBank.

Massive infection of a song thrush by Mesocestoides sp. (Cestoda) tetrathyridia that genetically match acephalic metacestodes causing lethal peritoneal larval cestodiasis in domesticated mammals

“…had just slightly higher divergence in this locus; these included M. corti (GenBank: AF286984 [38]) (with a divergence of 0.3 ± 0.3%) and M. litteratus (GenBank: DQ643000 [40]) (with a divergence of 1.0 ± 0.4%). The 12S rDNA of the analyzed species was also highly similar to those from larvae that were previously isolated from life-threatening infections of dogs in Turkey and identified as M. corti HM011122 [41], with a divergence of 0.7 ± 0.5%, and JN572111 [42], with a divergence of 0.7 ± 0.9%. However, these tetrathyridia clearly do not represent M. corti as other individuals, identified as M. corti by Kashiide et al (GenBank: AB848990 [25]) and Nakao (GenBank: AB031363; unpubl.)…”

“…2c). Aypak et al [42] reported abundant acephalic metacestodes alongside numerous empty calcified cysts of a diameter less than 6 mm that were aggregated into multiple free aggregates of 15 mm in diameter in the peritoneum of an examined dog. The dog manifested normocytic normochromic anemia with low red blood cell count and low hemoglobin, and neutrophilic leukocytosis.…”

Kanine peritoneale larvale Zestodose durch Mesocestoides spp.

“…Die Amplikons wurden sequenziert (LGC Genomics GmbH, Berlin) und per BLAST-Suche mit bekannten Gensequenzen (GenBank-Datenbanken) verglichen. Es ergab sich eine hohe Übereinstimmung (97-99%) zu Mesocestoides-Gensequenzen, die von Hunden und Katzen mit PLZ aus der Türkei (12S rRNA [1,32]), Spanien (nukleäre rRNA-Sequenz [11] bzw. Italien (COI-Sequenz [14]) stammen.…”

“…Über das Vorkommen der PLZ bei Hunden und Katzen liegen Berichte aus Nordamerika (3,4,7,29), Europa (1,2,9,11,14,23,27,(30)(31)(32) und Japan (15) vor. Dabei gibt es über die Häufigkeit in Patientenpopulationen nur ungenügende Informationen.…”

Nematode and cestode eggs scattered with cats-dogs feces and significance of public health in Samsun, Turkey

“…These animals host a variety of helminth species (15,33). There are many studies about the helminths of cats and dogs living in Turkey and approximately 20 species in cats and 40 species in dogs, a total of 60 helminth species have been reported up to now (5,15,36,37). Some of these parasites, particularly taenids cause economic losses in slaughtered ruminants and Toxocara spp.…”