A case of cutaneous T cell lymphoma with gastrointestinal stromal tumor.

千晴, 近藤; 修身, 大圓; 温, 佐藤; 悠紀子, 新田

doi:10.5227/skincancer.17.94

Cited by 1 publication

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“…Using an organic polymer, we are also able to test cations with a wider range of charges than metal ions alone. These molecules are also non-toxic and easily synthesized with a range of oligomer lengths 69,70 . Importantly, K n composed of L-lysine residues (as opposed to D-lysine residues) may also be degraded by cells, allowing for matrix reorganization in vitro independent of cellular degradation of the PA nanofibers.…”

Section: Pa Self-assembly and Gelationmentioning

confidence: 99%

“…To investigate how G' impacts neuronal differentiation, we encapsulated human iPSCderived DA neurons at 40 days post-differentiation in PA-1 and gelled the resulting solution with the chloride salts of K 4 (10mM), K 10 (4 mM), or Ca 2+ (20 mM) to create 3D gels. The total concentration of lysine monomers was kept constant in K n -based gels in order to control for potential differences in cell viability and growth due to potential lysine toxicity 24,69,90 . We also plated DA neurons on poly-D-lysine (PDL)/laminin coated coverslips as a positive control to ensure batch-to-batch consistency as well as on coverslips covered with only PLL.…”

Section: G' Controls Neuronal Phenotypementioning

confidence: 99%

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Gelator Length Precisely Tunes Supramolecular Hydrogel Stiffness and Neuronal Phenotype in 3D Culture

Godbe

Freeman

Burbulla

et al. 2020

ACS Biomater. Sci. Eng.

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The brain is one of the softest tissues in the body with storage moduli (G') that range from hundreds to thousands of pascals (Pa) depending upon the anatomic region. Furthermore, pathological processes such as injury, aging and disease can cause subtle changes in the mechanical properties throughout the central nervous system. However, these changes in mechanical properties lie within an extremely narrow range of moduli and there is great interest in understanding their effect on neuron biology. We report here the design of supramolecular hydrogels based on anionic peptide amphiphile nanofibers using oligo-L-lysines of different molecular lengths to precisely tune gel stiffness over the range of interest and found that G' increases by 10.5 Pa for each additional lysine monomer in the oligo-L-lysine chain. We found that small changes in storage modulus on the order of 70 Pa significantly affect survival, neurite growth and tyrosine hydroxylase-positive population in dopaminergic neurons derived from induced pluripotent stem cells. The work reported here offers a strategy to tune mechanical stiffness of hydrogels for use in 3D neuronal cell cultures and transplantation matrices for neural regeneration.

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Section: Pa Self-assembly and Gelationmentioning

confidence: 99%

Section: G' Controls Neuronal Phenotypementioning

confidence: 99%