A case-control study on the association of idiopathic recurrent pregnancy loss with autoantibodies against β2-glycoprotein I and annexin V

Zammiti, Walid; Mtiraoui, Nabil; Kallel, Choumous; Mercier, É.; Almawi, Wassim Y.; Mahjoub, Touhami

doi:10.1530/rep.1.01060

Cited by 34 publications

(29 citation statements)

References 30 publications

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“…In terms of the clinical importance of these antibodies, many authors suggest that a-A5 are an additional diagnostic marker in patients with different thrombotic conditions, reproductive problems and autoimmune diseases [2,9,15,19,28,31,32,38].…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic significance of anti-annexin-A5 antibody determination

2010

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AbstractAnti-annexin A5 antibodies are directed against annexin A5 — a phospholipid-binding protein that belongs to the ubiquitous annexin family. These antibodies were first discovered in 1994 by Matsuda et al. in women with recurrent fetal loss or preeclampsia and in patients with systemic lupus erythematosus and positive lupus anticoagulant and/or anticardiolipin antibodies. Since then anti-annexin A5 antibodies have been the focus of research. In addition to their well known prothrombotic and procoagulant activities the authors discuss the involvement of these antibodies in the pathogenesis of antiphospholipid syndrome, recurrent pregnancy loss, systemic lupus erythematosus and other immune and non-immune disorders. Controversial reports are presented and a possible interpretation of the results is given. The authors suggest the significance of anti-annexin A5 antibodies as an additional diagnostic marker and discuss the necessity of more extensive research on their clinical significance.

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Section: Discussionmentioning

confidence: 99%

“…The latter strongly correlated with the presence of recurrent miscarriages. In 2006, Zammiti et al [28] found that positive a-A5 and anti-b2GPI antibodies are independent risk factors for recurrent pregnancy loss.…”

Section: Significance Of Anti-annexin-a5mentioning

confidence: 99%

Diagnostic significance of anti-annexin-A5 antibody determination

2010

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“…Nevertheless, serum levels of aβ2GP1 may be significantly elevated in aCL-negative patients with APS. A close correlation has been demonstrated between the presence of aβ2GPI-IgM and the occurrence of abortions during an early phase of pregnancy (17,18). Annexin V is one of the most significant placental anticoagulant proteins that can be detected in a reduced quantity from placentas of mothers with APS (15,16).…”

Section: Pregnancy Morbidity Amentioning

confidence: 95%

“…The risk of abortions during the late phase of pregnancy is particularly high in the presence of IgG isotype aANX antibodies (17,18). In addition, in the first trimester of pregnancy, aPLs may induce disorders of placentation and embryonal implantation by directly inhibiting hormone production and invasion of trophoblasts (15).…”

Section: Pregnancy Morbidity Amentioning

confidence: 99%

Antibodies and autoimmune diseases in relation to reproductive failures

Kovács¹

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IV. Kovacs M., Hartwig M., Aleksza M., Tihanyi M., Nagy T., Vajda Gy., Daru J., XVII. Bodorkos I., Aleksza M., Horvath E., Dancza T., Farkas A., Gyorkos A., Kovacs M., Tiboly M., Toth P., Gasztonyi B.: Haemotherapy and its serological aspects of patients suffering from immunohaematological diseases. Blood Reviews, Suppl., 21, 119., 2007. IF: 5.922XVIII. Farkas A., Kovacs M., Gyorkos A., Milics M., Nagy Gy., Tarnok F.: Serious clinical manifestations of Systemic lupus erythematosus -case report. Magyar Belorv.

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“…Moreover, it is suspected that anti-annexin V Ab may interfere with annexin V functions and exerts a detrimental role leading to thrombosis and/or vascular occlusion. 13 Nevertheless, there has been conflicting evidence regarding the correlation of Ab against annexin V with disease, with some studies indicating an association with clinical manifestations such as increased risk of pregnancy losses or thrombosis [14][15][16][17][18][19] whereas others have found this not to be the case. …”

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confidence: 99%

Quantification of autoantibodies to annexin V in plasma by an "in house" sandwich ELISA

Pelusa¹,

Valdés²,

Daniele³

et al. 2012

J. Braz. Chem. Soc.

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Foi desenvolvido e validado um ELISA tipo sanduíche "in house" para a quantificação de anticorpos (Ac) anti-anexina V em plasma. Os parâmetros de validação estudados foram: (i) precisão, expressado como coeficiente de variação (CV) inter-e intra-ensaio, (ii) exatidão, expressado como porcentagem de desvio entre o valor obtido e o valor real, (iii) limite de detecção (LOD), avaliado a partir do branco de reagentes e (iv) robusteza, obtida através da introdução propositada de pequenas variações em diferentes parâmetros. Além disso, a técnica "in house" foi comparada com um método comercial. Encontrou-se que ambos CV foram < 20%, a exatidão foi de 100 ± 20%, o limite de detecção foi menor que 1 U mL -1 e as pequenas variações na técnica não produziram variações significativas nos resultados. A comparação com o método comercial mostrou uma correlação aceitável. Concluiu-se que o método desenvolvido cumpre satisfatoriamente com os parâmetros de padronização e validação para imuno-análise.An "in house" sandwich ELISA for the quantification of plasma anti-annexin V antibodies was developed and validated. The validation parameters studied were: (i) precision, expressed either as the intra-or the inter-assay coefficient of variation (CV), (ii) exactitude, expressed as the percentage deviation between the obtained value and the real value, (iii) limit of detection (LOD), evaluated from the reagents blank and (iv) robustness, obtained by deliberately introducing slight variations in different parameters. Also, a comparison between the "in house" technique and a commercial method was performed. The research revealed that both CV were < 20%, exactitude was within the 100 ± 20% range, limit of detection was below 1 U mL -1 and that slight variations in the technique did not produce any significant variations in the results. Comparison with the commercial method showed an acceptable correlation. It was concluded that the method developed here satisfactorily accomplishes the parameters of standardization and validation for an immunoassay.Keywords: ELISA, annexin V, antibodies, anti-phospholipid syndrome, foetal losses IntroductionAnnexins belong to a family of proteins that are able to bind to negatively charged phospholipids and membrane bilayers through calcium dependent interactions. Though their fine structure has been well described, their functions have not been clearly identified yet.1 Like others, annexins constitute a group of ubiquitous cytoplasmic proteins involved in signal transduction. 2Annexin V is a 320-amino acid-residue, 36-kDa-protein that is folded into a planar cyclic arrangement of four repeats with each repeat composed of five alpha-helical segments. 3,4 It is expressed in various cell types, including placental trophoblasts and vascular endothelial cells. This protein is highly expressed in an apparently constitutive manner by placental trophoblasts and is displayed Quantification of Autoantibodies to Annexin V in Plasma by an "In House" Sandwich ELISA J. Braz. Chem. Soc. 454 on the apical membrane o...

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A case-control study on the association of idiopathic recurrent pregnancy loss with autoantibodies against β2-glycoprotein I and annexin V

Cited by 34 publications

References 30 publications

Diagnostic significance of anti-annexin-A5 antibody determination

Diagnostic significance of anti-annexin-A5 antibody determination

Antibodies and autoimmune diseases in relation to reproductive failures

Quantification of autoantibodies to annexin V in plasma by an "in house" sandwich ELISA

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