A Case-Control Study and Meta-Analysis of the Association of eNOS rs1799983 SNP with Stroke Risk

Abstract

The endothelial nitric oxide synthase (eNOS) rs1799983 polymorphism is known to increase the risk towards stroke, but data is under-reported in Malaysian population. Therefore, this study sought to investigate this association in a Malaysian population and in a comprehensive meta-analysis. Genotyping of the eNOS rs1799983 polymorphism was performed for 241 Malaysians using a hydrolysis probe. Odd ratio with 95% confidence interval was calculated. Meta-analysis was conducted using the Comprehensive Meta-Analysis software ver. 2.2.064. A p-value less than 0.05 was considered statistically significant. Overall, our results showed that the presence of eNOS rs1799983-T allele increases the risk towards stroke, particularly in males, fast-food goers and Malaysian Chinese. The meta-analysis showed that the rs1799983 polymorphism is significantly associated with an increase ischemic stroke risk in the recessive and allelic models. After stratified with population, these associations remain significant in the Asian population but not in the Caucasian population. In summary, this study establishes a significant relationship between the eNOS rs1799983 polymorphism with gender, lifestyle and ethnicity differences towards stroke risk in the Malaysian population. In addition, our meta-analysis suggests that the eNOS rs1799983 polymorphism is associated with an increase risk of ischemic stroke.

Association between eNOS rs1799983 (894G>T) Polymorphism with Cancer and Stroke Risk: A meta-analysis

“…36 studies were screened, and 21 studies were excluded for various reasons. Ten other studies(da Costa Escobar Piccoli et al, 2012;Jang et al, 2013;Rah et al, 2013;Akhter et al, 2014;Kang et al, 2014; Özçelik et al, 2014;Ben Chaaben et al, 2015;Hung et al, 2019;Lee, 2019;Tsay et al, 2019) were also excluded because of unavailable genotype frequency. Six studies(Hao, Montiel and Huang, 2010;Yao et al, 2013; Guo, 2014;Zhao et al, 2014;Abedinzadeh et al, 2020;Akbar et al, 2022) were not included in the analysis because they were meta-analysis studies.…”