A cascade extraction of active phycocyanin and fatty acids from Galdieria phlegrea

Imbimbo, Paola; Romanucci, Valeria; Pollio, Antonino; Fontanarosa, Carolina; Amoresano, Angela; Zarrelli, Armando; Olivieri, Giuseppe; Monti, Daria Maria

doi:10.1007/s00253-019-10154-0

Cited by 20 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is performed by using solvents at boiling temperature and ambient pressure, and even if it requires high amount of solvents and a long extraction time, it provides high yields and does not affect the bioactivity of the extracted molecules. We recently reported a Soxhlet extraction with chloroform to methanol (2:1) to obtain lipids from Galdieria phlegrea (Imbimbo et al 2019). C. vulgaris Heptane 57.5 ± 0.5 (Minyak et al 2017) This procedure allowed obtaining a recovery of 79 ± 26% (w/w of dry biomass) of lipids starting from the dried biomass (Imbimbo et al 2019).…”

Section: Soxhlet Extractionmentioning

confidence: 99%

“…We recently reported a Soxhlet extraction with chloroform to methanol (2:1) to obtain lipids from Galdieria phlegrea (Imbimbo et al 2019). C. vulgaris Heptane 57.5 ± 0.5 (Minyak et al 2017) This procedure allowed obtaining a recovery of 79 ± 26% (w/w of dry biomass) of lipids starting from the dried biomass (Imbimbo et al 2019). Yusuff reported an oil extraction performed by Soxhlet from the green microalga Chlorophyta sp.…”

Section: Soxhlet Extractionmentioning

confidence: 99%

“…We recently reported a process intensification to obtain three different high-value molecules in a biorefinery approach (Imbimbo et al 2019). In particular, we improved lipid extraction as the third step of the cascade process.…”

Section: Compressed Fluid Extractionsmentioning

confidence: 99%

See 2 more Smart Citations

Towards green extraction methods from microalgae learning from the classics

Imbimbo

D’Elia

Liberti

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

This article is made publicly available in the institutional repository of Wageningen University and Research, under the terms of article 25fa of the Dutch Copyright Act, also known as the Amendment Taverne. This has been done with explicit consent by the author.Article 25fa states that the author of a short scientific work funded either wholly or partially by Dutch public funds is entitled to make that work publicly available for no consideration following a reasonable period of time after the work was first published, provided that clear reference is made to the source of the first publication of the work.This publication is distributed under The Association of Universities in the Netherlands (VSNU) 'Article 25fa implementation' project. In this project research outputs of researchers employed by Dutch Universities that comply with the legal requirements of Article 25fa of the Dutch Copyright Act are distributed online and free of cost or other barriers in institutional repositories. Research outputs are distributed six months after their first online publication in the original published version and with proper attribution to the source of the original publication.You are permitted to download and use the publication for personal purposes. All rights remain with the author(s) and / or copyright owner(s) of this work. Any use of the publication or parts of it other than authorised under article 25fa of the Dutch Copyright act is prohibited. Wageningen University & Research and the author(s) of this publication shall not be held responsible or liable for any damages resulting from your (re)use of this publication.

show abstract

Section: Soxhlet Extractionmentioning

confidence: 99%

Section: Soxhlet Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Towards green extraction methods from microalgae learning from the classics

Imbimbo

D’Elia

Liberti

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…References Heterotrophic growth Yes Yes Gross & Schnarrenberger, 1995;Oesterhelt et al, 1999;Schonknecht et al, 2013;Moriyama et al, 2015;Sloth et al, 2017 C-PC production Yes Yes Schmidt et al, 2005;Sloth et al, 2006, Sloth et al, 2017Graverholt & Eriksen, 2007;Wan et al, 2016;Carfagna et al, 2018;Wang et al, 2019;Rahman et al, 2020 C-PC extraction Yes Yes Sørensen et al, 2013;Moon et al, 2014;Rahman et al, 2017;Imbimbo et al, 2019 Waste stream treatment Yes N.A. Kurano et al, 1995;Selvaratnam et al, 2014;Minoda et al, 2015;Vítová et al, Ohta et al, 1998;Matsuzaki et al, 2004;Barbier et al, 2005;Schonknecht et al, phototrophically, has a cell wall, and has not yet been accessible to genetic modification.…”

Section: Galdieria Cyanidioschyzonmentioning

confidence: 99%

“…In recent years, the interest in photosynthetic unicellular organisms as a sustainable resource for biotechnological applications has increased significantly (Doron, Segal, & Shapira, 2016;Eichler-Stahlberg et al, 2009;Guzmán-Zapata et al, 2016;Imbimbo et al, 2019;Varshney et al, 2015). Research and development efforts have gradually expanded the microalgal biotechnology repertoire, but also accentuated the limitations thereof.…”

Section: Introductionmentioning

confidence: 99%

Exploiting the potential of Cyanidiales as a valuable resource for biotechnological applications

et al. 2020

View full text Add to dashboard Cite

The biotechnological uses of algae and cyanobacteria have been widely discussed in the context of climate change and consequent efforts to circularize economies, minimize carbon release and reuse waste streams. Their great potential in bioproduction and bioremediation has barely been exploited, particularly for the well-characterized red algae Galdieria sulphuraria and Cyanidioschyzon merolae. These and other Cyanidiales are excellent candidates for biotechnological enhancement and metabolic engineering for a broad spectrum of applications including the production of biofuels and thermostable colourants. In particular, extremophily, such as growth at thermophilic temperaturesup to 60°Cand at low pH and high salinity, make these algae unusually resistant to contamination and pathogens, and therefore potentially more commercially viable. We review existing applications of the Cyanidiales, as well as their available molecular tools. Their varied nutritional demands, from the broad heterotrophy of G. sulphuraria to strongly autotrophic C. merolae, along with their ability to grow to high densities, confer great potential as expression hosts. We also discuss the deficiencies that must be overcome to unlock further applications and ultimately to embed thermophilic red algae into a framework of circular and sustainable economic activity relying on bio-based sources.

show abstract