A cascade amplification approach for visualization of telomerase activity in living cells

Yan, Liwen; Hui, Jingjing; Liu, Yiran; Guo, Yuehua; Liu, Lu; Ding, Lin; Ju, Huangxian

doi:10.1016/j.bios.2016.07.102

Cited by 38 publications

(25 citation statements)

References 39 publications

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“…In addition, Ding demonstrated the integration of nucleic acid-based signal amplification with a molecular beacon for a intracellular telomerase assay. 55 …”

Section: In Vivo Imaging Of Intracellular Telomerase Activitymentioning

confidence: 99%

Sensing telomerase: From in vitro detection to in vivo imaging

Lijuan

Tang

et al. 2017

Chem. Sci.

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“…In addition, Ding demonstrated the integration of nucleic acid-based signal amplification with a molecular beacon for a intracellular telomerase assay. 55 …”

Section: In Vivo Imaging Of Intracellular Telomerase Activitymentioning

confidence: 99%

Sensing telomerase: From in vitro detection to in vivo imaging

Lijuan

Tang

et al. 2017

Chem. Sci.

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“…Our hairpin‐mediated extension system was further applied to sense telomerase activity in situ after treatment of different telomerase‐targeting anticancer agents. Telomerase sense oligodeoxynucleotide (SODN), telomerase antisense oligodeoxynucleotide (ASODN), and epigallocatechin gallate (EGCG) were used as model drugs to inhibit telomerase activity in living HeLa cells (the sequences of SODN and ASODN are provided in the Supporting Information) . SODN and ASODN can represent large biomolecule drugs, whereas EGCG represents a small‐molecule drug.…”

Section: Resultsmentioning

confidence: 99%

Live‐Cell Sensing of Telomerase Activity by Using Hybridization‐Sensitive Fluorescent Oligonucleotide Probes

et al. 2019

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Live‐cell sensing of telomerase activity with simple and efficient strategies remains a challenging target. In this work, a strategy for telomerase sensing by using hybridization‐sensitive fluorescent oligonucleotide probes is reported. In the presence of telomerase and dNTPs, the designed supporting strand was extended and generated the hairpin structure that catalyzed the next telomerase extending reaction. The special extension mechanism increased the local concentration of another supporting strand and telomerase, which resulted in enhanced telomerase activity. The hybridization‐sensitive oligonucleotide probes bound to the hairpin catalyst and generated turn‐on fluorescence. This method realized the sensing of telomerase activity in HeLa cell extract with a detection limit below 1.6×10−6 IU μL−1. The real‐time in situ observation of telomerase extension was achieved in living HeLa cells. This strategy has been applied to monitor the efficiency of telomerase‐targeting anticancer drugs in situ.

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“…Biomarkers including mRNA, miRNA, metal ions, and telomerase, have been imaged with CHA based strategies. Scientists have employed modified nucleic acids or NPs to protect the system components.…”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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A cascade amplification approach for visualization of telomerase activity in living cells

Cited by 38 publications

References 39 publications

Sensing telomerase: From in vitro detection to in vivo imaging

Sensing telomerase: From in vitro detection to in vivo imaging

Live‐Cell Sensing of Telomerase Activity by Using Hybridization‐Sensitive Fluorescent Oligonucleotide Probes

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

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